The Aedes aegypti larval transcriptome: a comparative perspective with emphasis on trypsins and the domain structure of peritrophins.

Abstract:

:The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adults (AG) and in both stages (SG). A total of 913 genes (5.4% of the transcript complement) are LG, including ion transporters and cuticle proteins that are important for ion homeostasis and defense. From a starting set of 245 genes encoding the trypsin domain, we identified 66 putative LG, AG, and SG trypsins by manual curation. Phylogenetic analyses showed that AG trypsins are divergent from their larval counterparts (LG), grouping with blood-induced trypsins from Anopheles gambiae and Simulium vittatum. These results support the hypothesis that blood-feeding arose only once, in the ancestral Culicomorpha. Peritrophins are proteins that interlock chitin fibrils to form the peritrophic membrane (PM) that compartmentalizes the food in the midgut. These proteins are recognized by having chitin-binding domains with 6 conserved Cys and may also present mucin-like domains (regions expected to be highly O-glycosylated). PM may be formed by a ring of cells (type 2, seen in Ae. aegypti larvae and Drosophila melanogaster) or by most midgut cells (type 1, found in Ae. aegypti adult and Tribolium castaneum). LG and D. melanogaster peritrophins have more complex domain structures than AG and T. castaneum peritrophins. Furthermore, mucin-like domains of peritrophins from T. castaneum (feeding on rough food) are lengthier than those of adult Ae. aegypti (blood-feeding). This suggests, for the first time, that type 1 and type 2 PM may have variable molecular architectures determined by different peritrophins and/or ancillary proteins, which may be partly modulated by diet.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Venancio TM,Cristofoletti PT,Ferreira C,Verjovski-Almeida S,Terra WR

doi

10.1111/j.1365-2583.2008.00845.x

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

33-44

issue

1

eissn

0962-1075

issn

1365-2583

pii

IMB845

journal_volume

18

pub_type

杂志文章
  • Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28S rDNA sequences.

    abstract::The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1994.tb00142.x

    authors: Campbell BC,Steffen-Campbell JD,Werren JH

    更新日期:1993-01-01 00:00:00

  • Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species.

    abstract::Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monope...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01048.x

    authors: Saikia M,Singh YT,Bhattacharya A,Mazumdar-Leighton S

    更新日期:2011-02-01 00:00:00

  • Pyrosequencing the Manduca sexta larval midgut transcriptome: messages for digestion, detoxification and defence.

    abstract::The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00936.x

    authors: Pauchet Y,Wilkinson P,Vogel H,Nelson DR,Reynolds SE,Heckel DG,ffrench-Constant RH

    更新日期:2010-02-01 00:00:00

  • The complete mitochondrial genome sequence of the Mormon cricket (Anabrus simplex: Tettigoniidae: Orthoptera) and an analysis of control region variability.

    abstract::The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00721.x

    authors: Fenn JD,Cameron SL,Whiting MF

    更新日期:2007-04-01 00:00:00

  • Functional characterization of Musca glutamate- and GABA-gated chloride channels expressed independently and coexpressed in Xenopus oocytes.

    abstract::Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00680.x

    authors: Eguchi Y,Ihara M,Ochi E,Shibata Y,Matsuda K,Fushiki S,Sugama H,Hamasaki Y,Niwa H,Wada M,Ozoe F,Ozoe Y

    更新日期:2006-12-01 00:00:00

  • Molecular characterization of a prophenoloxidase cDNA from the malaria mosquito Anopheles stephensi.

    abstract::Some refractory anopheline mosquitoes are capable of killing Plasmodium, the causative agent of malaria, by melanotic encapsulation of invading ookinetes. Phenoloxidase (PO) appears to be involved in the formation of melanin and toxic metabolites in the surrounding capsule. A cDNA encoding Anopheles stephensi propheno...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2000.00169.x

    authors: Cui L,Luckhart S,Rosenberg R

    更新日期:2000-04-01 00:00:00

  • Cloning of a family of serine protease genes from the cat flea Ctenocephalides felis.

    abstract::Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDN...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1999.810011.x

    authors: Gaines PJ,Sampson CM,Rushlow KE,Stiegler GL

    更新日期:1999-02-01 00:00:00

  • Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth.

    abstract::The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST of Bombyx mori (bmGSTO) was shown to be induced after exposure to various environmental stresses. A soluble form of recombinant protein (rbmGSTO) was functionally ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2011.01073.x

    authors: Yamamoto K,Teshiba S,Shigeoka Y,Aso Y,Banno Y,Fujiki T,Katakura Y

    更新日期:2011-06-01 00:00:00

  • Cloning and characterization of a serine protease from the human malaria vector, Anopheles gambiae.

    abstract::The nucleotide and deduced amino acid sequence of a serine protease (AgSp24D) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 271 amino acid protein that contains the conserved serine, histidine and aspartic acid residues found in serine proteases, and has the highest identity to ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1997.00193.x

    authors: Han YS,Salazar CE,Reese-Stardy SR,Cornel A,Gorman MJ,Collins FH,Paskewitz SM

    更新日期:1997-11-01 00:00:00

  • Multifunctional cellulase enzymes are ancestral in Polyneoptera.

    abstract::Many hemimetabolous insects produce their own cellulase enzymes from the glycoside hydrolase family 9, first observed in termites and cockroaches. Phasmatodea have multiple cellulases, some of which are multifunctional and can degrade xylan or xyloglucan. To discover when these abilities evolved, we identified cellula...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12614

    authors: Shelomi M,Wipfler B,Zhou X,Pauchet Y

    更新日期:2020-02-01 00:00:00

  • Bacterial community structure and diversity in the gut of the muga silkworm, Antheraea assamensis (Lepidoptera: Saturniidae), from India.

    abstract::The muga silkworm, Antheraea assamensis, is exclusively present in the northeastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture-dependent techniques, generic identification using 16S ribosomal RNA probes, diversity analysis and qualitative screening f...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12495

    authors: Gandotra S,Kumar A,Naga K,Bhuyan PM,Gogoi DK,Sharma K,Subramanian S

    更新日期:2018-10-01 00:00:00

  • A venom metalloproteinase from the parasitic wasp Eulophus pennicornis is toxic towards its host, tomato moth (Lacanobia oleracae).

    abstract::Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00864.x

    authors: Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

    更新日期:2009-04-01 00:00:00

  • Genetic analysis of the xenobiotic resistance-associated ABC gene subfamilies of the Lepidoptera.

    abstract::Some ATP-binding cassette (ABC) transporters of subfamilies B, C and G confer resistance to xenobiotics including insecticides. We identified genes of these subfamilies expressed by the lepidopterans Trichoplusia ni and Bombyx mori. The B. mori genome includes eight, six and 13 ABC-B, -C and -G genes, respectively, wh...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01064.x

    authors: Labbé R,Caveney S,Donly C

    更新日期:2011-04-01 00:00:00

  • Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex.

    abstract::The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM is believed to act as a damage sensor for the ICL-blocked replication fork and also as a molecular platform for FA core complex assembly and interaction with Bloom's syndrome (BS) complex...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12072

    authors: Sugahara R,Mon H,Lee JM,Kusakabe T

    更新日期:2014-04-01 00:00:00

  • Isolation and characterization of a termite transferrin gene up-regulated on infection.

    abstract::PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00381.x

    authors: Thompson GJ,Crozier YC,Crozier RH

    更新日期:2003-02-01 00:00:00

  • Identification of Y-chromosome scaffolds of the Queensland fruit fly reveals a duplicated gyf gene paralogue common to many Bactrocera pest species.

    abstract::Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12602

    authors: Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

    更新日期:2019-12-01 00:00:00

  • Pyrosequencing and characterization of immune response genes from the American dog tick, Dermacentor variabilis (L.).

    abstract::Ticks continue to be a threat to animal and human health, and new and novel control strategies are needed for ticks and tick-borne pathogens. The characterization of the tick-pathogen interface and the tick immune response to microbial infections is fundamental toward the formulation of new control strategies for tick...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01037.x

    authors: Jaworski DC,Zou Z,Bowen CJ,Wasala NB,Madden R,Wang Y,Kocan KM,Jiang H,Dillwith JW

    更新日期:2010-10-01 00:00:00

  • Wing development genes of the pea aphid and differential gene expression between winged and unwinged morphs.

    abstract::Little is known about when, how or even if the wing development gene network elucidated in Drosophila is deployed in direct-developing insects. Here we identify the wing development genes (as determined in Drosophila) of the pea aphid (Acyrthosiphon pisum), which produces winged or unwinged adults in response to envir...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00935.x

    authors: Brisson JA,Ishikawa A,Miura T

    更新日期:2010-03-01 00:00:00

  • Induction of the white egg 3 mutant phenotype by injection of the double-stranded RNA of the silkworm white gene.

    abstract::Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2002.00328.x

    authors: Quan GX,Kanda T,Tamura T

    更新日期:2002-06-01 00:00:00

  • Preservation of a transgenic strain of the sawfly, Athalia rosae (Hymenoptera) by artificial fertilization using cryopreserved sperm.

    abstract::Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00526.x

    authors: Hatakeyama M,Sumitani M

    更新日期:2005-01-01 00:00:00

  • Variation in the salivary peptide, maxadilan, from species in the Lutzomyia longipalpis complex.

    abstract::Maxadilan is an approximately 7kDa peptide that occurs in the saliva of the sand fly Lutzomyia longipalpis. This peptide is a potent vasodilator and may also have immunomodulatory effects related to the pathogenesis of leishmanial infections. Variation in the primary DNA and inferred amino acid sequence of maxadilan i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1999.820267.x

    authors: Lanzaro GC,Lopes AH,Ribeiro JM,Shoemaker CB,Warburg A,Soares M,Titus RG

    更新日期:1999-05-01 00:00:00

  • Genetic mapping of genes conferring permethrin resistance in the malaria vector, Anopheles gambiae.

    abstract::Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.0962-1075.2004.00495.x

    authors: Ranson H,Paton MG,Jensen B,McCarroll L,Vaughan A,Hogan JR,Hemingway J,Collins FH

    更新日期:2004-08-01 00:00:00

  • Downregulation of dystrophin expression in pupae of the whitefly Bemisia tabaci inhibits the emergence of adults.

    abstract::The whitefly Bemisia tabaci is a major pest to agriculture. Adults are able to fly for long distances and to colonize staple crops, herbs and ornamentals, and to vector viruses belonging to several important taxonomic groups. During their early development, whiteflies mature from eggs through several nymphal stages (i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12579

    authors: Bar L,Czosnek H,Sobol I,Ghanim M,Hariton Shalev A

    更新日期:2019-10-01 00:00:00

  • Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species.

    abstract::Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00464.x

    authors: Foy BD,Myles KM,Pierro DJ,Sanchez-Vargas I,Uhlírová M,Jindra M,Beaty BJ,Olson KE

    更新日期:2004-02-01 00:00:00

  • Molecular characterization of a gene encoding juvenile hormone esterase in the red flour beetle, Tribolium castaneum.

    abstract::Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01019.x

    authors: Tsubota T,Minakuchi C,Nakakura T,Shinoda T,Shiotsuki T

    更新日期:2010-08-01 00:00:00

  • The distribution of microsatellites in the Nasonia parasitoid wasp genome.

    abstract::Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00915.x

    authors: Pannebakker BA,Niehuis O,Hedley A,Gadau J,Shuker DM

    更新日期:2010-02-01 00:00:00

  • Analysis of intraspecies polymorphism in the ribosomal DNA cluster of the cockroach Blattella germanica.

    abstract::HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2000.00175.x

    authors: Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal C

    更新日期:2000-04-01 00:00:00

  • Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera.

    abstract::The western corn rootworm (WCR), a major pest of maize, is notorious for rapidly adapting biochemically, behaviourally and developmentally to a variety of control methods. Despite much effort, the genetic basis of WCR adaptation remains a mystery. Since transformation-based applications such as transposon tagging and ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12305

    authors: Chu F,Klobasa W,Wu P,Pinzi S,Grubbs N,Gorski S,Cardoza Y,Lorenzen MD

    更新日期:2017-08-01 00:00:00

  • The POU homeodomain transcription factor POUM2 and broad complex isoform 2 transcription factor induced by 20-hydroxyecdysone collaboratively regulate vitellogenin gene expression and egg formation in the silkworm Bombyx mori.

    abstract::Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12315

    authors: Lin Y,Liu H,Yang C,Gu J,Shen G,Zhang H,Chen E,Han C,Zhang Y,Xu Y,Wu J,Xia Q

    更新日期:2017-10-01 00:00:00

  • Reassessment of 79B actin gene expression in the abdomen of adult Drosophila melanogaster.

    abstract::The expression pattern of the 79B actin gene in Drosophila melanogaster has been inferred previously by means of a reporter gene in which 79B actin promoter sequences drive the lacZ coding sequences. Although the 79B actin gene is expressed primarily in muscles of the thorax and first abdominal segment of the adults o...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1997.00176.x

    authors: Ohshima S,Villarimo C,Gailey DA

    更新日期:1997-08-01 00:00:00