Abstract:
:HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polymorphisms in three cockroach strains show that individuals from different populations may have different HindIII rDNA patterns, whereas individuals within populations exhibit relatively similar rDNA patterns. We suggest that HindIII restriction fragment polymorphisms within cockroach ribosomal DNA will be a valuable tool for measuring population-level parameters within and between natural cockroach populations.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal Cdoi
10.1046/j.1365-2583.2000.00175.xsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
217-22issue
2eissn
0962-1075issn
1365-2583pii
imb175journal_volume
9pub_type
杂志文章abstract::Ticks continue to be a threat to animal and human health, and new and novel control strategies are needed for ticks and tick-borne pathogens. The characterization of the tick-pathogen interface and the tick immune response to microbial infections is fundamental toward the formulation of new control strategies for tick...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01037.x
更新日期:2010-10-01 00:00:00
abstract::Although the Hymenoptera represent a remarkably diverse and socioeconomically important group that is of considerable interest in genome biology, they remain understudied in terms of genome size. This study reports new genome size estimates for 89 species of ants, bees and wasps, representing 17 families and four supe...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.00992.x
更新日期:2010-06-01 00:00:00
abstract::The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thu...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12521
更新日期:2018-12-01 00:00:00
abstract::Cyclobutane pyrimidine dimer (CPD) photolyases repair ultraviolet (UV)-induced DNA damage using blue light. To get insight in the origin of baculovirus CPD photolyase (phr) genes, homologues in the lepidopteran insects Chrysodeixis chalcites, Spodoptera exigua and Trichoplusia ni were identified and characterized. Lep...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2011.01076.x
更新日期:2011-08-01 00:00:00
abstract::Some refractory anopheline mosquitoes are capable of killing Plasmodium, the causative agent of malaria, by melanotic encapsulation of invading ookinetes. Phenoloxidase (PO) appears to be involved in the formation of melanin and toxic metabolites in the surrounding capsule. A cDNA encoding Anopheles stephensi propheno...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00169.x
更新日期:2000-04-01 00:00:00
abstract::The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2002.00373.x
更新日期:2002-12-01 00:00:00
abstract::A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila me...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1993.tb00122.x
更新日期:1993-01-01 00:00:00
abstract::Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases (CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12678
更新日期:2021-02-01 00:00:00
abstract::In mammals, the mitogen-activated protein (MAP) kinase pathway is one of the four major signalling systems that respond to stress and inflammatory stimuli. A full-length cDNA corresponding to Aedes aegypti MAP kinase kinase 3 (AaMEK3) was cloned and sequenced. It is 1.7 kb and contains an open reading frame of 334 ami...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00444.x
更新日期:2003-12-01 00:00:00
abstract::Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. T...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.0962-1075.2001.00300.x
更新日期:2001-12-01 00:00:00
abstract::A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00917.x
更新日期:2009-11-01 00:00:00
abstract::The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1998.00063.x
更新日期:1998-08-01 00:00:00
abstract::Some ATP-binding cassette (ABC) transporters of subfamilies B, C and G confer resistance to xenobiotics including insecticides. We identified genes of these subfamilies expressed by the lepidopterans Trichoplusia ni and Bombyx mori. The B. mori genome includes eight, six and 13 ABC-B, -C and -G genes, respectively, wh...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01064.x
更新日期:2011-04-01 00:00:00
abstract::We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2005.00585.x
更新日期:2005-10-01 00:00:00
abstract::To begin to explore the molecular dynamics of rickettsial tick interaction, subtractive hybridization was used to screen mRNAs in Rickettsia montanensis-infected and uninfected Dermacentor variabilis. We isolated 30 cDNA fragments, 22 of which were up-regulated and eight were down-regulated in response to rickettsial ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00400.x
更新日期:2003-04-01 00:00:00
abstract::Four cytochrome P450 genes, CYP6B8, CYP6B9, CYP6B27 and CYP6B28, exist in the Helicoverpa zea genome as two pairs of paralogs that evolved from gene duplication and 5'-polar gene conversion events. RT-PCR gel blot analyses have shown that all of these genes are expressed constitutively in midguts of all larval instars...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2002.00341.x
更新日期:2002-08-01 00:00:00
abstract::Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2008.00780.x
更新日期:2008-02-01 00:00:00
abstract::Many hemimetabolous insects produce their own cellulase enzymes from the glycoside hydrolase family 9, first observed in termites and cockroaches. Phasmatodea have multiple cellulases, some of which are multifunctional and can degrade xylan or xyloglucan. To discover when these abilities evolved, we identified cellula...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12614
更新日期:2020-02-01 00:00:00
abstract::A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00755.x
更新日期:2007-10-01 00:00:00
abstract::Speckled (Spc), an X-ray-induced lethal mutant of Bombyx mori, exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in females. Here, we report the morphological characterization and molecular mapping of the Spc mutant. Morphological investigations revealed that the epiderma...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12205
更新日期:2016-04-01 00:00:00
abstract::A high number of (GT)n and (CT)n microsatellites were isolated from a partial genomic library of primitively eusocial wasp, Vespula rufa. On average, (GT)n and (CT)n repeats occur in the genome approximately every 8 kb and 2.5 kb respectively. This is the highest density of dinucleotide microsatellites reported in the...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1995.tb00019.x
更新日期:1995-08-01 00:00:00
abstract::Loxosceles intermedia venom comprises a complex mixture of proteins, glycoproteins and low molecular mass peptides that act synergistically to immobilize envenomed prey. Analysis of a venom-gland transcriptome from L. intermedia revealed that knottins, also known as inhibitor cystine knot peptides, are the most abunda...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12268
更新日期:2017-02-01 00:00:00
abstract::We recently showed that the Masculinizer gene (Masc) plays a primary role in sex determination in the lepidopteran model insect Bombyx mori. However, it remains unknown whether this Masc protein-dependent sex determination system is conserved amongst lepidopteran insects or within the family Bombycidae. Here we cloned...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12181
更新日期:2015-10-01 00:00:00
abstract::The hormone 20-hydroxyecdysone is fundamental for regulating moulting and metamorphosis in immature insects, and it plays a role in physiological regulation in adult insects. This hormone acts by binding and activating a receptor, the ecdysone receptor, which is part of the nuclear receptor gene superfamily. Here, we ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12696
更新日期:2021-01-17 00:00:00
abstract::Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12532
更新日期:2019-02-01 00:00:00
abstract::Age-dependent division of labour in honeybees was shown to be connected to sensory response thresholds. Foragers show a higher gustatory responsiveness than nurse bees. It is generally assumed that nutrition-related signalling pathways underlie this behavioural plasticity. Here, one important candidate gene is the for...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12520
更新日期:2018-12-01 00:00:00
abstract::The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12226
更新日期:2016-08-01 00:00:00
abstract::We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00425.x
更新日期:2003-08-01 00:00:00
abstract::Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDN...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1999.810011.x
更新日期:1999-02-01 00:00:00
abstract::We previously demonstrated that Amblyomma americanum tick serine protease inhibitor 6 (AamS6) was secreted into the host during tick feeding and that both its mRNA and protein were ubiquitously and highly expressed during the first 3 days of tick feeding. This study demonstrates that AamS6 is a cross-class inhibitor o...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12024
更新日期:2013-06-01 00:00:00