Bacterial community structure and diversity in the gut of the muga silkworm, Antheraea assamensis (Lepidoptera: Saturniidae), from India.

Abstract:

:The muga silkworm, Antheraea assamensis, is exclusively present in the northeastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture-dependent techniques, generic identification using 16S ribosomal RNA probes, diversity analysis and qualitative screening for enzyme activities, our studies have identified a number of bacterial isolates, viz. Bacillus spp., Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas stutzeri, Acinetobacter sp. and Alcaligens sp., inhabiting the gut of the muga silkworm. Analysis of the culturable bacterial community from the gut of An. assamensis revealed that Bacillus (54%) was the predominant bacterial genus followed by Serratia (24%), Pseudomonas (10%) and Alcaligens (6%). Significant differences in the Shannon-Wiener (H') and Simpson (D) diversity indices of gut bacteria were recorded for An. assamensis collected from different regions. H' and D values were found to be highest for An. assamensis from the Titabar region (H' = 4.73 ± 0.43; D = 10.00 ± 0.11) and lowest for individuals from the Mendipathar region (H' = 2.1 ± 0.05; D = 0.04 ± 0.00) of northeastern India. Qualitative screening for enzyme activities identified about 26 gut bacterial isolates having significantly higher cellulose, amylase and lipase activities. These isolates probably contribute to the digestion and nutrition of their host insect, An. assamensis.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Gandotra S,Kumar A,Naga K,Bhuyan PM,Gogoi DK,Sharma K,Subramanian S

doi

10.1111/imb.12495

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

603-619

issue

5

eissn

0962-1075

issn

1365-2583

journal_volume

27

pub_type

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