Isolation and characterization of a termite transferrin gene up-regulated on infection.

Abstract:

:PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino acids) for any insect transferrin characterized to date. Cysteines and residues comprising putative iron-binding sites are conserved in both N- and C-terminal lobes, suggesting structural and functional similarity to diferric vertebrate transferrins. A quantitative PCR assay confirmed a significant increase in transferrin expression following infection, suggesting its up-regulation is part of the innate immune response. However, codon-based tests for selection among known insect transferrins revealed only a small proportion of codon-sites positively selected. Thus, unlike certain vertebrate transferrin lineages, no widespread evidence for pathogen-mediated positive selection was detected at this locus.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Thompson GJ,Crozier YC,Crozier RH

doi

10.1046/j.1365-2583.2003.00381.x

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

1-7

issue

1

eissn

0962-1075

issn

1365-2583

pii

381

journal_volume

12

pub_type

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