Levels and activity of cyclic guanosine monophosphate-dependent protein kinase in nurse and forager honeybees.

Abstract:

:Age-dependent division of labour in honeybees was shown to be connected to sensory response thresholds. Foragers show a higher gustatory responsiveness than nurse bees. It is generally assumed that nutrition-related signalling pathways underlie this behavioural plasticity. Here, one important candidate gene is the foraging gene, which encodes a cyclic guanosine monophosphate-dependent protein kinase (PKG). Several roles of members of this enzyme family were analysed in vertebrates. They own functions in important processes such as growth, secretion and neuronal adaptation. Honeybee foraging messenger RNA expression is upregulated in the brain of foragers. In vivo activation of PKG can modulate gustatory responsiveness. We present for the first time PKG protein level and activity data in the context of social behaviour and feeding. Protein level was significantly higher in brains of foragers than in those of nurse bees, substantiating the role of PKG in behavioural plasticity. However, enzyme activity did not differ between behavioural roles. The mediation of feeding status appears independent of PKG signalling. Neither PKG content nor enzyme activity differed between starved and satiated individuals. We suggest that even though nutrition-related pathways are surely involved in controlling behavioural plasticity, which involves changes in PKG signalling, mediation of satiety itself is independent of PKG.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Thamm M,Sturm K,Schlossmann J,Scheiner R

doi

10.1111/imb.12520

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

815-823

issue

6

eissn

0962-1075

issn

1365-2583

journal_volume

27

pub_type

杂志文章
  • Evolutionary variation in gene expression is associated with dimorphism in eusocial vespid wasps.

    abstract::Phenotypic diversity is frequently generated by differences in gene expression. In this study, we addressed the relationship between homology in gene expression and phenotype among four species of eusocial wasps. Specifically, we investigated the evolution of caste-specific and sex-specific gene expression patterns as...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01021.x

    authors: Hunt BG,Goodisman MA

    更新日期:2010-10-01 00:00:00

  • Roles of the Laodelphax striatellus Down syndrome cell adhesion molecule in Rice stripe virus infection of its insect vector.

    abstract::The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12226

    authors: Zhang F,Li Q,Chen X,Huo Y,Guo H,Song Z,Cui F,Zhang L,Fang R

    更新日期:2016-08-01 00:00:00

  • Functional characterization of Musca glutamate- and GABA-gated chloride channels expressed independently and coexpressed in Xenopus oocytes.

    abstract::Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00680.x

    authors: Eguchi Y,Ihara M,Ochi E,Shibata Y,Matsuda K,Fushiki S,Sugama H,Hamasaki Y,Niwa H,Wada M,Ozoe F,Ozoe Y

    更新日期:2006-12-01 00:00:00

  • Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes.

    abstract::Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2012.01141.x

    authors: Adelman ZN,Anderson MA,Liu M,Zhang L,Myles KM

    更新日期:2012-06-01 00:00:00

  • Cowpea bruchid midgut transcriptome response to a soybean cystatin--costs and benefits of counter-defence.

    abstract::The insect digestive system is the first line of defence protecting cells and tissues of the body from a broad spectrum of toxins and antinutritional factors in its food. To gain insight into the nature and breadth of genes involved in adaptation to dietary challenge, a collection of 20 352 cDNAs was prepared from the...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00854.x

    authors: Chi YH,Salzman RA,Balfe S,Ahn JE,Sun W,Moon J,Yun DJ,Lee SY,Higgins TJ,Pittendrigh B,Murdock LL,Zhu-Salzman K

    更新日期:2009-02-01 00:00:00

  • PCR primers for the amplification of four insect mitochondrial gene fragments.

    abstract::Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect m...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1995.tb00028.x

    authors: Kambhampati S,Smith PT

    更新日期:1995-11-01 00:00:00

  • Raalin, a transcript enriched in the honey bee brain, is a remnant of genomic rearrangement in Hymenoptera.

    abstract::We identified a predicted compact cysteine-rich sequence in the honey bee genome that we called 'Raalin'. Raalin transcripts are enriched in the brain of adult honey bee workers and drones, with only minimum expression in other tissues or in pre-adult stages. Open-reading frame (ORF) homologues of Raalin were identifi...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2012.01138.x

    authors: Tirosh Y,Morpurgo N,Cohen M,Linial M,Bloch G

    更新日期:2012-06-01 00:00:00

  • Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28S rDNA sequences.

    abstract::The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1994.tb00142.x

    authors: Campbell BC,Steffen-Campbell JD,Werren JH

    更新日期:1993-01-01 00:00:00

  • Developmental expression and evolution of hexamerin and haemocyanin from Folsomia candida (Collembola).

    abstract::Haemocyanins constitute a group of copper-containing respiratory proteins, and hexamerins were derived from hexapod haemocyanin but lost the ability to transport oxygen and serve as storage proteins. Although hexamerins have been reported in most insect species, none of them has been identified in Collembola, one of t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12585

    authors: Liang Y,Xie W,Luan YX

    更新日期:2019-10-01 00:00:00

  • piggyBac-like elements in the tobacco budworm, Heliothis virescens (Fabricius).

    abstract::We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00653.x

    authors: Wang J,Ren X,Miller TA,Park Y

    更新日期:2006-08-01 00:00:00

  • Identification of Y-chromosome scaffolds of the Queensland fruit fly reveals a duplicated gyf gene paralogue common to many Bactrocera pest species.

    abstract::Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12602

    authors: Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

    更新日期:2019-12-01 00:00:00

  • A role for alcohol dehydrogenase in the Drosophila immune response?

    abstract::In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00543.x

    authors: Schlenke TA,McKean KA

    更新日期:2005-04-01 00:00:00

  • An estimate of the number of serine protease genes expressed in sheep blowfly larvae (Lucilia cuprina).

    abstract::A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1994.tb00157.x

    authors: Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

    更新日期:1994-05-01 00:00:00

  • Amblyomma americanum tick saliva serine protease inhibitor 6 is a cross-class inhibitor of serine proteases and papain-like cysteine proteases that delays plasma clotting and inhibits platelet aggregation.

    abstract::We previously demonstrated that Amblyomma americanum tick serine protease inhibitor 6 (AamS6) was secreted into the host during tick feeding and that both its mRNA and protein were ubiquitously and highly expressed during the first 3 days of tick feeding. This study demonstrates that AamS6 is a cross-class inhibitor o...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12024

    authors: Mulenga A,Kim T,Ibelli AM

    更新日期:2013-06-01 00:00:00

  • Inducible- and constitutive-type transcript variants of kakusei , a novel non-coding immediate early gene, in the honeybee brain.

    abstract::We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00821.x

    authors: Kiya T,Kunieda T,Kubo T

    更新日期:2008-09-01 00:00:00

  • Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L.

    abstract::To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00911.x

    authors: Tadano H,Yamazaki Y,Takeuchi H,Kubo T

    更新日期:2009-11-01 00:00:00

  • Genetic structuring of boll weevil populations in the US based on RAPD markers.

    abstract::Abstract Randomly amplified polymorphic DNA (RAPD) analysis was performed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from eighteen locations across eight US states and north-east Mexico. Sixty-seven reproducible bands from six random primers were analysed for genetic va...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.0962-1075.2004.00487.x

    authors: Kim KS,Sappington TW

    更新日期:2004-06-01 00:00:00

  • The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid.

    abstract::The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thu...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12521

    authors: Sun ZJ,Li ZX

    更新日期:2018-12-01 00:00:00

  • Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina.

    abstract::We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expresse...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.0962-1075.2004.00468.x

    authors: Yamamoto-Kihara M,Kotani E

    更新日期:2004-04-01 00:00:00

  • Genes of the antioxidant system of the honey bee: annotation and phylogeny.

    abstract::Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00695.x

    authors: Corona M,Robinson GE

    更新日期:2006-10-01 00:00:00

  • Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in dipteran insects.

    abstract::The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1998.00063.x

    authors: Jordan TV,Shike H,Boulo V,Cedeno V,Fang Q,Davis BS,Jacobs-Lorena M,Higgs S,Fryxell KJ,Burns JC

    更新日期:1998-08-01 00:00:00

  • Sequence and evolution of a hexamerin from the ant Camponotus festinatus.

    abstract::In the ant Camponotus festinatus, two different hexamerins accumulate stage-specifically during the late larval period and at various times in adults. These hexamerins serve as storage proteins and play important roles in brood nourishment and colony founding. We report an analysis of the cDNA sequence of C. festinatu...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2000.00204.x

    authors: Martínez T,Burmester T,Veenstra JA,Wheeler D

    更新日期:2000-08-01 00:00:00

  • The scarlet eye colour gene of the tephritid fruit fly: Bactrocera tryoni and the nature of two eye colour mutations.

    abstract::A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00410.x

    authors: Zhao JT,Bennett CL,Stewart GJ,Frommer M,Raphael KA

    更新日期:2003-06-01 00:00:00

  • Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae.

    abstract::Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae, we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding fu...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2002.00350.x

    authors: Ranson H,Nikou D,Hutchinson M,Wang X,Roth CW,Hemingway J,Collins FH

    更新日期:2002-10-01 00:00:00

  • Polymorphisms and fluctuations in copy number of amplified esterase genes in Culex pipiens mosquitoes.

    abstract::In Culex pipiens mosquitoes, A2 esterase alleles are co-amplified with B2 esterase alleles in response to selection with organophosphate insecticides. In this study the amplified A2 and B2 sequences were compared between twelve strains from four continents by restriction mapping. The restriction maps were almost ident...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1998.00077.x

    authors: Callaghan A,Guillemaud T,Makate N,Raymond M

    更新日期:1998-08-01 00:00:00

  • Characterization and expression of the odorant-binding protein 7 gene in Anopheles stephensi and comparative analysis among five mosquito species.

    abstract::Odorant-binding proteins (OBPs) are important molecular players in insect olfaction, which has a great influence on the host-seeking behaviour of mosquitoes and other disease vectors. The mRNA level of the Anopheles gambiae Obp7 gene (Agam-Obp7) is higher in the adult female antennae and is slightly reduced in the fem...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00837.x

    authors: Sengul MS,Tu Z

    更新日期:2008-12-01 00:00:00

  • Neuropeptide Y-like signalling and nutritionally mediated gene expression and behaviour in the honey bee.

    abstract::Previous research has led to the idea that derived traits can arise through the evolution of novel roles for conserved genes. We explored whether neuropeptide Y (NPY)-like signalling, a conserved pathway that regulates food-related behaviour, is involved in a derived, nutritionally-related trait, the division of labou...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2011.01068.x

    authors: Ament SA,Velarde RA,Kolodkin MH,Moyse D,Robinson GE

    更新日期:2011-06-01 00:00:00

  • Differential regulation of ribosomal protein gene expression in Aedes aegypti mosquitoes before and after the blood meal.

    abstract::In fat body of the mosquito, Aedes aegypti, a cycle of ribosome accumulation and degradation accompanies synthesis of the yolk protein precursor, vitellogenin. Here we compare the transcription and translation of ribosomal proteins rpS6, rpL8 and rpL34, relative to rRNA and vitellogenin genes in Aedes aegypti fat body...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2000.00226.x

    authors: Niu LL,Fallon AM

    更新日期:2000-12-01 00:00:00

  • An expansion of the genome size dataset for the insect order Hymenoptera, with a first test of parasitism and eusociality as possible constraints.

    abstract::Although the Hymenoptera represent a remarkably diverse and socioeconomically important group that is of considerable interest in genome biology, they remain understudied in terms of genome size. This study reports new genome size estimates for 89 species of ants, bees and wasps, representing 17 families and four supe...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.00992.x

    authors: Ardila-Garcia AM,Umphrey GJ,Gregory TR

    更新日期:2010-06-01 00:00:00

  • Downregulation of dystrophin expression in pupae of the whitefly Bemisia tabaci inhibits the emergence of adults.

    abstract::The whitefly Bemisia tabaci is a major pest to agriculture. Adults are able to fly for long distances and to colonize staple crops, herbs and ornamentals, and to vector viruses belonging to several important taxonomic groups. During their early development, whiteflies mature from eggs through several nymphal stages (i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12579

    authors: Bar L,Czosnek H,Sobol I,Ghanim M,Hariton Shalev A

    更新日期:2019-10-01 00:00:00