Methods for the generation of heritable germline mutations in the disease vector Culex quinquefasciatus using clustered regularly interspaced short palindrome repeats-associated protein 9.

abstract：:Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...

journal_title：Insect molecular biology

authors： Quan GX,Kanda T,Tamura T

更新日期：2002-06-01 00:00:00

abstract：:Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, L...

journal_title：Insect molecular biology

authors： Yang Y,Zhao X,Niu N,Zhao Y,Liu W,Moussian B,Zhang J

更新日期：2020-12-01 00:00:00

abstract：:The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...

journal_title：Insect molecular biology

authors： Fenn JD,Cameron SL,Whiting MF

更新日期：2007-04-01 00:00:00

abstract：:RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major ...

journal_title：Insect molecular biology

authors： Balakrishna Pillai A,Nagarajan U,Mitra A,Krishnan U,Rajendran S,Hoti SL,Mishra RK

更新日期：2017-04-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prop...

journal_title：Insect molecular biology

authors： Paskewitz SM,Reese-Stardy S,Gorman MJ

更新日期：1999-08-01 00:00:00

abstract：:We have used a PCR assay to screen 404 insects and related arthropods for mariner elements using primers corresponding to amino acids conserved between the mariner elements of Drosophila mauritiana and a moth, Hyalophora cecropia. Potential mariner elements were detected in sixty-three species, representing ten insect...

journal_title：Insect molecular biology

authors： Robertson HM,MacLeod EG

更新日期：1993-01-01 00:00:00

abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

journal_title：Insect molecular biology

authors： Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

更新日期：1994-08-01 00:00:00

abstract：:Wolbachia is an intracellular bacterium that has aroused intense interest because of its ability to alter the biology of its host in diverse ways. In the two-spotted spider mite, Tetranychus urticae, Wolbachia can induce complex cytoplasmic incompatibility (CI) phenotypes and fitness changes, although little is known ...

journal_title：Insect molecular biology

authors： Zhang YK,Ding XL,Rong X,Hong XY

更新日期：2015-02-01 00:00:00

abstract：:In the ant Camponotus festinatus, two different hexamerins accumulate stage-specifically during the late larval period and at various times in adults. These hexamerins serve as storage proteins and play important roles in brood nourishment and colony founding. We report an analysis of the cDNA sequence of C. festinatu...

journal_title：Insect molecular biology

authors： Martínez T,Burmester T,Veenstra JA,Wheeler D

更新日期：2000-08-01 00:00:00

abstract：:The P450 monooxygenases of insects are important in the metabolism of numerous endogenous and exogenous compounds. However, identity of the P450 isoform(s) involved in these reactions is rarely known. A critical first step in the identification of important P450s is the cloning and sequencing of their genes. Toward th...

journal_title：Insect molecular biology

authors： Kasai S,Scott JG

更新日期：2001-06-01 00:00:00

abstract：:In the present study, we characterized a full-length cDNA encoding a putative iron-binding protein transferrin from the lepidopteran Mediterranean flour moth (EkTrf, 2397 bp). The putative EkTrf is 683 amino acids with a molecular mass of approximately 76 kDa. The deduced amino acid sequence showed significant homolog...

journal_title：Insect molecular biology

authors： Guz N,Kilincer N,Aksoy S

更新日期：2012-04-01 00:00:00

abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....

journal_title：Insect molecular biology

authors： Pauchet Y,Wilkinson P,Vogel H,Nelson DR,Reynolds SE,Heckel DG,ffrench-Constant RH

更新日期：2010-02-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Droso...

journal_title：Insect molecular biology

authors： Tarnay JN,Szeri F,Iliás A,Annilo T,Sung C,Le Saux O,Váradi A,Dean M,Boyd CD,Robinow S

更新日期：2004-10-01 00:00:00

abstract：:The muga silkworm, Antheraea assamensis, is exclusively present in the northeastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture-dependent techniques, generic identification using 16S ribosomal RNA probes, diversity analysis and qualitative screening f...

journal_title：Insect molecular biology

authors： Gandotra S,Kumar A,Naga K,Bhuyan PM,Gogoi DK,Sharma K,Subramanian S

更新日期：2018-10-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00

abstract：:PCR primers designed from the alpha-esterase gene cluster of Drosophila melanogaster have been used to isolate fragments from eight esterase genes in the Australian sheep blowfly, Lucilia cuprina. Phylogenetic analysis suggests that three are homologues of the alpha E7, alpha E8 and alpha E9 genes of the alpha-esteras...

journal_title：Insect molecular biology

authors： Newcomb RD,East PD,Russell RJ,Oakeshott JG

更新日期：1996-08-01 00:00:00

abstract：:The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM is believed to act as a damage sensor for the ICL-blocked replication fork and also as a molecular platform for FA core complex assembly and interaction with Bloom's syndrome (BS) complex...

journal_title：Insect molecular biology

authors： Sugahara R,Mon H,Lee JM,Kusakabe T

更新日期：2014-04-01 00:00:00

abstract：:Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...

journal_title：Insect molecular biology

authors： Hens K,Lemey P,Macours N,Francis C,Huybrechts R

更新日期：2004-12-01 00:00:00

abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

journal_title：Insect molecular biology

authors： Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

更新日期：1994-05-01 00:00:00

abstract：:The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...

journal_title：Insect molecular biology

authors： Zhang F,Li Q,Chen X,Huo Y,Guo H,Song Z,Cui F,Zhang L,Fang R

更新日期：2016-08-01 00:00:00

abstract：:The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thu...

journal_title：Insect molecular biology

authors： Sun ZJ,Li ZX

更新日期：2018-12-01 00:00:00

abstract：:The Asian rice gall midge, Orseolia oryzae, is a fast evolving, damaging pest of rice. Understanding the underlying molecular mechanism of interaction between the gall midge and rice will help in devising strategies to control and manage the pest. The present study aims to identify rice-responsive genes in the gall mi...

journal_title：Insect molecular biology

authors： Sinha DK,Bentur JS,Nair S

更新日期：2011-10-01 00:00:00

abstract：:We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference be...

journal_title：Insect molecular biology

authors： Ma L,Gerenday A,Coley KM,Fallon AM

更新日期：2006-04-01 00:00:00

abstract：:Myosuppressins are a family of the FMRFamide-like peptides. They have been characterized in many insects and shown to inhibit visceral muscle contraction. Rhodnius prolixus possesses an unusual myosuppressin in that the typical FLRFamide C-terminal motif is unique and ends with FMRFamide. In the present study, we isol...

journal_title：Insect molecular biology

authors： Lee D,James TE,Lange AB

更新日期：2015-02-01 00:00:00

abstract：:Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...

journal_title：Insect molecular biology

authors： Duan SG,Li DZ,Wang MQ

更新日期：2019-02-01 00:00:00

abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...

journal_title：Insect molecular biology

authors： Marshall SD,Gatehouse LN,Becher SA,Christeller JT,Gatehouse HS,Hurst MR,Boucias DG,Jackson TA

更新日期：2008-06-01 00:00:00

abstract：:Although the Hymenoptera represent a remarkably diverse and socioeconomically important group that is of considerable interest in genome biology, they remain understudied in terms of genome size. This study reports new genome size estimates for 89 species of ants, bees and wasps, representing 17 families and four supe...

journal_title：Insect molecular biology

authors： Ardila-Garcia AM,Umphrey GJ,Gregory TR

更新日期：2010-06-01 00:00:00

abstract：:Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

journal_title：Insect molecular biology

authors： Zandawala M,Hamoudi Z,Lange AB,Orchard I

更新日期：2015-04-01 00:00:00

abstract：:Transcriptional activity of nuclear receptors is the result of transactivation capability and the concentration of the receptor protein. The concentration of ecdysteroid receptor (EcR) isoforms, constitutively expressed in mammalian CHO cells, is dependent on a number of factors. As shown previously, ligand binding st...

journal_title：Insect molecular biology

authors： Schauer S,Burster T,Spindler-Barth M

更新日期：2012-06-01 00:00:00