An estimate of the number of serine protease genes expressed in sheep blowfly larvae (Lucilia cuprina).

Abstract:

:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser residues found in all serine proteases. PCR products from entire first-instar larval cDNA, or from third-instar larval salivary glands or cardia, generated using a microscale RT-PCR method, were cloned into a plasmid vector. Comparison of the restriction fragment patterns of PCR products generated from the three different sources suggests a highly diverse tissue-specific pattern of serine protease expression in this organism. Detailed analysis of the restriction fragment patterns of sixty-nine randomly selected clones from entire first-instar larvae revealed forty-nine different classes of PCR product. Maximum likelihood analysis of these data indicate that between 125 and 220 different serine protease genes are expressed in first-instar larvae of L. cuprina. DNA sequence analysis of ten randomly-selected clones, derived from the three tissue sources, indicated that all ten encoded serine protease gene fragments. A frequently occurring PCR product, generated from both first-instar total cDNA and third-instar cardia cDNA, showed 73% amino acid identity to a digestive protease expressed in Drosophila melanogaster larval gut cells.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

doi

10.1111/j.1365-2583.1994.tb00157.x

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

105-15

issue

2

eissn

0962-1075

issn

1365-2583

journal_volume

3

pub_type

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