A new aminopeptidase from diamondback moth provides evidence for a gene duplication event in Lepidoptera.

Abstract:

:We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a signal peptide which directs processing by the endoplasmic reticulum, a C-terminal hydrophobic region satisfying the criterion for a GPI anchor and cleavage, and the possibility of an O-glycosylated rigid stalk attached to the GPI anchor. PxAPN-A is more closely related to MsAPN-1 than it is to another aminopeptidase recently reported from P. xylostella. Sequence comparisons with other species suggests that at least one aminopeptidase gene duplication occurred in an ancestral lepidopteran.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Chang WX,Gahan LJ,Tabashnik BE,Heckel DG

doi

10.1046/j.1365-2583.1999.820171.x

subject

Has Abstract

pub_date

1999-05-01 00:00:00

pages

171-7

issue

2

eissn

0962-1075

issn

1365-2583

journal_volume

8

pub_type

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