Bombyx mori epidermal growth factor receptor is required for nucleopolyhedrovirus replication.

Abstract:

:Baculovirus-host interactions are important models for studying the biological control of lepidopteran pests. Research on baculovirus-host interactions has focussed on baculovirus manipulation of cellular signalling pathways, including the extracellular signal-regulated kinase (ERK) and phosphatidylinositol-3-kinases/protein kinase B (PI3K/Akt) signalling pathways. However, the mechanism underlying ERK and PI3K/Akt activation and function in response to baculovirus infection remains poorly understood. Here, we demonstrated that baculovirus activated the Bombyx mori ERK and PI3K/Akt signalling pathways via the B. mori epidermal growth factor receptor (BmEGFR). To further characterize the function of the BmEGFR/ERK signalling pathway in baculovirus replication, we calculated genome-wide changes in kinase-chromatin interactions for ERK after baculovirus infection using chromatin immunoprecipitation followed by high-throughput sequencing. A Gene Ontology analysis showed that virus infection had effects on the biological regulation, cellular process and metabolic process pathways. Moreover, ERK was shown to regulate the transcription of late viral genes. Taken together, our results suggest that baculoviruses manipulate components of the host cell machinery for replication via modulation of the BmEGFR signalling pathway.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Jin S,Cheng T,Guo Y,Lin P,Zhao P,Liu C,Kusakabe T,Xia Q

doi

10.1111/imb.12386

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

464-477

issue

4

eissn

0962-1075

issn

1365-2583

journal_volume

27

pub_type

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