Abstract:
:Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, placed them within the gamma subdivision of the Proteobacteria, phylogenetically distinct from most members of the Rickettsiaceae which align with the alpha subdivision. Distinct multiple endogenous plasmids are harboured by GP01 and GM02, suggesting that the two isolates are different. Restriction mapping analysis showed that one of the conserved plasmids is present in high copy number and is at least 80 kb in size. A heterologous plasmid pSUP204, which contains the broad host range oriV replication origin, was used to transfect bacterial cultures. The symbiont GM02 was transformed, and it expressed plasmid encoded resistance to the antibiotics ampicillin, tetracycline and chloramphenicol. Transformation of these symbionts may provide a novel means for expressing anti-parasitic genes within tsetse populations.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Beard CB,O'Neill SL,Mason P,Mandelco L,Woese CR,Tesh RB,Richards FF,Aksoy Sdoi
10.1111/j.1365-2583.1993.tb00113.xsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
123-31issue
3eissn
0962-1075issn
1365-2583journal_volume
1pub_type
杂志文章abstract::Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00905.x
更新日期:2009-10-01 00:00:00
abstract::We have used a PCR assay to screen 404 insects and related arthropods for mariner elements using primers corresponding to amino acids conserved between the mariner elements of Drosophila mauritiana and a moth, Hyalophora cecropia. Potential mariner elements were detected in sixty-three species, representing ten insect...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1993.tb00132.x
更新日期:1993-01-01 00:00:00
abstract::Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicator...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1999.810119.x
更新日期:1999-02-01 00:00:00
abstract::Fast and reliable methods for assessing the mobility of the transposable element Minos have been developed. These methods are based on the detection of excision and insertion of Minos transposons from and into plasmids which are co-introduced into cells. Excision is detected by polymerase chain reaction (PCR) with app...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00183.x
更新日期:2000-06-01 00:00:00
abstract::Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monope...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01048.x
更新日期:2011-02-01 00:00:00
abstract::Culex pipiens quinquefasciatus Say and Culex pipiens pipiens Linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. The two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00707.x
更新日期:2007-04-01 00:00:00
abstract::Phosphofructokinase (PFK) is a rate-limiting enzyme in glycolysis, but its linkage with locomotion in termites is not well understood, despite the demonstrated involvement of this gene in the locomotion of different animals. Here, we investigated the effect of the pfk gene on locomotion in the subterranean termite Ret...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12672
更新日期:2021-02-01 00:00:00
abstract::Speckled (Spc), an X-ray-induced lethal mutant of Bombyx mori, exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in females. Here, we report the morphological characterization and molecular mapping of the Spc mutant. Morphological investigations revealed that the epiderma...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12205
更新日期:2016-04-01 00:00:00
abstract::Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00882.x
更新日期:2009-06-01 00:00:00
abstract::Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2005.00561.x
更新日期:2005-06-01 00:00:00
abstract::The nucleotide and deduced amino acid sequence of a serine protease (AgSp24D) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 271 amino acid protein that contains the conserved serine, histidine and aspartic acid residues found in serine proteases, and has the highest identity to ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1997.00193.x
更新日期:1997-11-01 00:00:00
abstract::Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00915.x
更新日期:2010-02-01 00:00:00
abstract::Insect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N-deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinot...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12524
更新日期:2019-02-01 00:00:00
abstract::Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2004.00526.x
更新日期:2005-01-01 00:00:00
abstract::A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00755.x
更新日期:2007-10-01 00:00:00
abstract::The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1994.tb00142.x
更新日期:1993-01-01 00:00:00
abstract::Horizontal transfer (HT), the exchange of genetic material between species, plays important roles in transposon biology and genome evolution. In this study, we provide the first documented example of a new Academ transposon involved in recent and distant HTs into the genomes of species belonging to seven different ord...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12225
更新日期:2016-06-01 00:00:00
abstract::The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00721.x
更新日期:2007-04-01 00:00:00
abstract::Abstract Randomly amplified polymorphic DNA (RAPD) analysis was performed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from eighteen locations across eight US states and north-east Mexico. Sixty-seven reproducible bands from six random primers were analysed for genetic va...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.0962-1075.2004.00487.x
更新日期:2004-06-01 00:00:00
abstract::Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01019.x
更新日期:2010-08-01 00:00:00
abstract::Nuclear ribosomal ITS2 and mitochondrial cytochrome oxidase DNA sequences were analysed from the Byturidae (Coleoptera), which includes the raspberry beetles. The secondary structure of ITS2 was plotted and interspecific changes analysed. Evidence for selection on simple sequence repeats within the ITS2 was found. Phy...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00266.x
更新日期:2001-06-01 00:00:00
abstract::Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2008.00798.x
更新日期:2008-06-01 00:00:00
abstract::A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00410.x
更新日期:2003-06-01 00:00:00
abstract::Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, L...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12665
更新日期:2020-12-01 00:00:00
abstract::Sensory neurone membrane proteins (SNMPs) function as essential cofactors for insect sex pheromone detection. In this study, we report two SNMPs in Ectropis obliqua Prout, a serious geometrid pest that produces typical Type-II sex pheromones. Sequence alignments and phylogenetic analyses showed that EoblSNMP1 and Eobl...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12555
更新日期:2019-06-01 00:00:00
abstract::A wide array of sensilla are distributed on insect antennae, and they play a variety of important roles. Rice planthoppers, destructive pests on rice, have a unique antenna sensilla structure called the 'sensory plaque organ'. The spineless (ss) gene encodes a bHLH-PAS transcription factor and plays a key role in ante...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12538
更新日期:2019-04-01 00:00:00
abstract::The pea aphid (Acyrthosiphon pisum) is the first whole genome sequenced insect with a hemimetabolic development and an emerging model organism for studies in ecology, evolution and development. The insect steroid moulting hormone 20-hydroxyecdysone (20E) controls and coordinates development in insects, especially the ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00957.x
更新日期:2010-03-01 00:00:00
abstract::To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00911.x
更新日期:2009-11-01 00:00:00
abstract::Little is known about when, how or even if the wing development gene network elucidated in Drosophila is deployed in direct-developing insects. Here we identify the wing development genes (as determined in Drosophila) of the pea aphid (Acyrthosiphon pisum), which produces winged or unwinged adults in response to envir...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00935.x
更新日期:2010-03-01 00:00:00
abstract::The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thu...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12521
更新日期:2018-12-01 00:00:00