Abstract:
:Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library from Culicoides nubeculosus linked to the analysis of abundant salivary gland proteins by mass spectrometry. Fifty-four novel proteins sequences are described including those of the enzymes maltase, hyaluronidase and two serine proteases demonstrated to be present in Culicoides salivary glands, as well as several members of the D7 family and protease inhibitors with putative anticoagulant activity. In addition, several families of abundant proteins with unknown function were identified including some of the major candidate allergens that cause insect bite hypersensitivity in horses.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Russell CL,Heesom KJ,Arthur CJ,Helps CR,Mellor PS,Day MJ,Torsteinsdottir S,Björnsdóttir TS,Wilson ADdoi
10.1111/j.1365-2583.2009.00882.xsubject
Has Abstractpub_date
2009-06-01 00:00:00pages
383-93issue
3eissn
0962-1075issn
1365-2583pii
IMB882journal_volume
18pub_type
杂志文章abstract::Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...
journal_title:Insect molecular biology
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abstract::A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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更新日期:2013-06-01 00:00:00
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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