Identification and isolation of cDNA clones encoding the abundant secreted proteins in the saliva proteome of Culicoides nubeculosus.

Abstract:

:Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library from Culicoides nubeculosus linked to the analysis of abundant salivary gland proteins by mass spectrometry. Fifty-four novel proteins sequences are described including those of the enzymes maltase, hyaluronidase and two serine proteases demonstrated to be present in Culicoides salivary glands, as well as several members of the D7 family and protease inhibitors with putative anticoagulant activity. In addition, several families of abundant proteins with unknown function were identified including some of the major candidate allergens that cause insect bite hypersensitivity in horses.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Russell CL,Heesom KJ,Arthur CJ,Helps CR,Mellor PS,Day MJ,Torsteinsdottir S,Björnsdóttir TS,Wilson AD

doi

10.1111/j.1365-2583.2009.00882.x

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

383-93

issue

3

eissn

0962-1075

issn

1365-2583

pii

IMB882

journal_volume

18

pub_type

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