Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor.

abstract：:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...

journal_title：Insect molecular biology

authors： Pedra JH,Brandt A,Westerman R,Lobo N,Li HM,Romero-Severson J,Murdock LL,Pittendrigh BR

更新日期：2003-08-01 00:00:00

abstract：:Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...

journal_title：Insect molecular biology

authors： Duan SG,Li DZ,Wang MQ

更新日期：2019-02-01 00:00:00

abstract：:In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...

journal_title：Insect molecular biology

authors： Watanabe T

更新日期：2019-12-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...

journal_title：Insect molecular biology

authors： Nagoshi RN,Meagher R

更新日期：2003-10-01 00:00:00

abstract：:Germline transformation of Drosophila melanogaster was attempted with the piggyBac gene-transfer system from the cabbage looper moth, Trichoplusia ni. Using a self-regulated transposase helper and a white marked vector, a transformation frequency of 1-3% per fertile G0 was obtained, similar to that previously achieved...

journal_title：Insect molecular biology

authors： Handler AM,Harrell RA 2nd

更新日期：1999-11-01 00:00:00

abstract：:Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...

journal_title：Insect molecular biology

authors： Lin Y,Liu H,Yang C,Gu J,Shen G,Zhang H,Chen E,Han C,Zhang Y,Xu Y,Wu J,Xia Q

更新日期：2017-10-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Droso...

journal_title：Insect molecular biology

authors： Tarnay JN,Szeri F,Iliás A,Annilo T,Sung C,Le Saux O,Váradi A,Dean M,Boyd CD,Robinow S

更新日期：2004-10-01 00:00:00

abstract：:Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...

journal_title：Insect molecular biology

authors： Adelman ZN,Anderson MA,Liu M,Zhang L,Myles KM

更新日期：2012-06-01 00:00:00

abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....

journal_title：Insect molecular biology

authors： Pauchet Y,Wilkinson P,Vogel H,Nelson DR,Reynolds SE,Heckel DG,ffrench-Constant RH

更新日期：2010-02-01 00:00:00

abstract：:Sperm usage was investigated in an instrumentally inseminated honeybee queen. Her progeny were examined in the first 3 months of the egg-laying period using a microsatellite marker. Frequencies of different subfamilies differed significantly from one month to another. However, there was no evidence for sperm displacem...

journal_title：Insect molecular biology

authors： Franck P,Coussy H,Le Conte Y,Solignac M,Garnery L,Cornuet JM

更新日期：1999-08-01 00:00:00

abstract：:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

journal_title：Insect molecular biology

authors： Wang J,Ren X,Miller TA,Park Y

更新日期：2006-08-01 00:00:00

abstract：:In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...

journal_title：Insect molecular biology

authors： Li F,Zhao X,Li M,He K,Huang C,Zhou Y,Li Z,Walters JR

更新日期：2019-12-01 00:00:00

abstract：:The biogenic amine receptor genes constitute an ancient and highly divergent family within the larger superfamily of G-protein-coupled receptors. These receptors play a central role in modulating nerve cell activity and thus behaviour. Because the honey bee offers numerous advantages for behavioural studies we endeavo...

journal_title：Insect molecular biology

authors： Ebert PR,Rowland JE,Toma DP

更新日期：1998-05-01 00:00:00

abstract：:Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

journal_title：Insect molecular biology

authors： Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

更新日期：1992-01-01 00:00:00

abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...

journal_title：Insect molecular biology

authors： Marshall SD,Gatehouse LN,Becher SA,Christeller JT,Gatehouse HS,Hurst MR,Boucias DG,Jackson TA

更新日期：2008-06-01 00:00:00

abstract：:Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

journal_title：Insect molecular biology

authors： Hatakeyama M,Sumitani M

更新日期：2005-01-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...

journal_title：Insect molecular biology

authors： Schlenke TA,McKean KA

更新日期：2005-04-01 00:00:00

abstract：:HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...

journal_title：Insect molecular biology

authors： Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal C

更新日期：2000-04-01 00:00:00

abstract：:To begin to explore the molecular dynamics of rickettsial tick interaction, subtractive hybridization was used to screen mRNAs in Rickettsia montanensis-infected and uninfected Dermacentor variabilis. We isolated 30 cDNA fragments, 22 of which were up-regulated and eight were down-regulated in response to rickettsial ...

journal_title：Insect molecular biology

authors： Mulenga A,Macaluso KR,Simser JA,Azad AF

更新日期：2003-04-01 00:00:00

abstract：:A birth-and-death evolutionary model for odorant receptor gene repertoires presumes the creation of repertoires with the capacity for high-level diversity and rapid ligand specificity change. This changes the recognised odour space, directly affecting fitness-related behaviours and ultimately affecting adaptation to n...

journal_title：Insect molecular biology

authors： Hill SR,Majeed S,Ignell R

更新日期：2015-08-01 00:00:00

abstract：:Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

journal_title：Insect molecular biology

authors： Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

更新日期：2019-12-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00

abstract：:cDNAs of Anopheles gambiae Defensin 2 (AgDef2), Defensin 3 (AgDef3) and Defensin 4 (AgDef4), identified in the genome sequence, have been characterized and their expression profiles investigated. In contrast to both typical defensins and insect antimicrobial peptides generally, the newly identified defensins were not ...

journal_title：Insect molecular biology

authors： Meredith JM,Hurd H,Lehane MJ,Eggleston P

更新日期：2008-04-01 00:00:00

abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...

journal_title：Insect molecular biology

authors： Vyazunova I,Wessley V,Kim M,Lan Q

更新日期：2007-06-01 00:00:00

abstract：:We aimed to understand the underlying mechanism that regulates successively expressed cuticular protein (CP) genes around pupation in Bombyx mori. Quantitative PCR was conducted to clarify the expression profile of CP genes and ecdysone-responsive transcription factor (ERTF) genes around pupation. Ecdysone pulse treat...

journal_title：Insect molecular biology

authors： Shahin R,Iwanaga M,Kawasaki H

更新日期：2016-04-01 00:00:00

abstract：:Insect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N-deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinot...

journal_title：Insect molecular biology

authors： Wu JJ,Chen ZC,Wang YW,Fu KY,Guo WC,Li GQ

更新日期：2019-02-01 00:00:00

abstract：:The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Med...

journal_title：Insect molecular biology

authors： Meyering-Vos M,Müller A

更新日期：2007-08-01 00:00:00

abstract：:The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...

journal_title：Insect molecular biology

authors： Campbell BC,Steffen-Campbell JD,Werren JH

更新日期：1993-01-01 00:00:00

abstract：:Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicator...

journal_title：Insect molecular biology

authors： Thibault ST,Luu HT,Vann N,Miller TA

更新日期：1999-02-01 00:00:00