Precise excision and transposition of piggyBac in pink bollworm embryos.

Abstract:

:Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicators of an element's ability to be mobilized in vivo and thus potentially serve as a transforming vector. We report that the transposable element piggyBac is capable of precise excision and transposition in the pink bollworm (Pectinophora gossypiella), a worldwide pest of cultivated cotton. Combined with a suitable marker gene, the piggyBac element may serve as a vector for germline transformation in this and (potentially) other lepidopteran species.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Thibault ST,Luu HT,Vann N,Miller TA

doi

10.1046/j.1365-2583.1999.810119.x

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

119-23

issue

1

eissn

0962-1075

issn

1365-2583

journal_volume

8

pub_type

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