Abstract:
:Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicators of an element's ability to be mobilized in vivo and thus potentially serve as a transforming vector. We report that the transposable element piggyBac is capable of precise excision and transposition in the pink bollworm (Pectinophora gossypiella), a worldwide pest of cultivated cotton. Combined with a suitable marker gene, the piggyBac element may serve as a vector for germline transformation in this and (potentially) other lepidopteran species.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Thibault ST,Luu HT,Vann N,Miller TAdoi
10.1046/j.1365-2583.1999.810119.xsubject
Has Abstractpub_date
1999-02-01 00:00:00pages
119-23issue
1eissn
0962-1075issn
1365-2583journal_volume
8pub_type
杂志文章abstract::Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2012.01145.x
更新日期:2012-08-01 00:00:00
abstract::In the ant Camponotus festinatus, two different hexamerins accumulate stage-specifically during the late larval period and at various times in adults. These hexamerins serve as storage proteins and play important roles in brood nourishment and colony founding. We report an analysis of the cDNA sequence of C. festinatu...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00204.x
更新日期:2000-08-01 00:00:00
abstract::Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2012.01158.x
更新日期:2012-10-01 00:00:00
abstract::In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1994.tb00175.x
更新日期:1994-11-01 00:00:00
abstract::Adaptive polymorphism may be common in immune system genes as co-evolutionary interactions foster diversity; either through ongoing positive selection (arms races), or balancing selection. DNA sequence diversity in two putative immune system genes was examined in species of the genus Anopheles and from Aedes aegypti. ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2005.00588.x
更新日期:2005-12-01 00:00:00
abstract::Cyclobutane pyrimidine dimer (CPD) photolyases repair ultraviolet (UV)-induced DNA damage using blue light. To get insight in the origin of baculovirus CPD photolyase (phr) genes, homologues in the lepidopteran insects Chrysodeixis chalcites, Spodoptera exigua and Trichoplusia ni were identified and characterized. Lep...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2011.01076.x
更新日期:2011-08-01 00:00:00
abstract::Sensory neurone membrane proteins (SNMPs) function as essential cofactors for insect sex pheromone detection. In this study, we report two SNMPs in Ectropis obliqua Prout, a serious geometrid pest that produces typical Type-II sex pheromones. Sequence alignments and phylogenetic analyses showed that EoblSNMP1 and Eobl...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12555
更新日期:2019-06-01 00:00:00
abstract::Some refractory anopheline mosquitoes are capable of killing Plasmodium, the causative agent of malaria, by melanotic encapsulation of invading ookinetes. Phenoloxidase (PO) appears to be involved in the formation of melanin and toxic metabolites in the surrounding capsule. A cDNA encoding Anopheles stephensi propheno...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00169.x
更新日期:2000-04-01 00:00:00
abstract::Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.0962-1075.2004.00495.x
更新日期:2004-08-01 00:00:00
abstract::We previously demonstrated that Amblyomma americanum tick serine protease inhibitor 6 (AamS6) was secreted into the host during tick feeding and that both its mRNA and protein were ubiquitously and highly expressed during the first 3 days of tick feeding. This study demonstrates that AamS6 is a cross-class inhibitor o...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12024
更新日期:2013-06-01 00:00:00
abstract::Phenotypic diversity is frequently generated by differences in gene expression. In this study, we addressed the relationship between homology in gene expression and phenotype among four species of eusocial wasps. Specifically, we investigated the evolution of caste-specific and sex-specific gene expression patterns as...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01021.x
更新日期:2010-10-01 00:00:00
abstract::Melanization constitutes an important component in various aspects of insect life, including cuticular sclerotization, egg-shell tanning, melanization of parasites and wound healing. Recently, a cDNA encoding prophenoloxidase (pro-PO), a key enzyme in the biosynthesis of melanotic material in insects, was cloned from ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00241.x
更新日期:2001-02-01 00:00:00
abstract::Lepidoptera is one of the most widespread insect orders and includes several agriculturally important insect species. Ecological success of the lepidopteran insects partly depends on their adaptive chemoreception tactics, which play an important role in the selection of hosts, egg-laying sites and mates. Members of th...
journal_title:Insect molecular biology
pub_type: 杂志文章,评审
doi:10.1111/imb.12246
更新日期:2016-10-01 00:00:00
abstract::Wolbachia is an intracellular bacterium that has aroused intense interest because of its ability to alter the biology of its host in diverse ways. In the two-spotted spider mite, Tetranychus urticae, Wolbachia can induce complex cytoplasmic incompatibility (CI) phenotypes and fitness changes, although little is known ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12128
更新日期:2015-02-01 00:00:00
abstract::Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.00999.x
更新日期:2010-08-01 00:00:00
abstract::Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00680.x
更新日期:2006-12-01 00:00:00
abstract::The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Med...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00737.x
更新日期:2007-08-01 00:00:00
abstract::The hormone 20-hydroxyecdysone is fundamental for regulating moulting and metamorphosis in immature insects, and it plays a role in physiological regulation in adult insects. This hormone acts by binding and activating a receptor, the ecdysone receptor, which is part of the nuclear receptor gene superfamily. Here, we ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12696
更新日期:2021-01-17 00:00:00
abstract::The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00429.x
更新日期:2003-10-01 00:00:00
abstract::In fat body of the mosquito, Aedes aegypti, a cycle of ribosome accumulation and degradation accompanies synthesis of the yolk protein precursor, vitellogenin. Here we compare the transcription and translation of ribosomal proteins rpS6, rpL8 and rpL34, relative to rRNA and vitellogenin genes in Aedes aegypti fat body...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00226.x
更新日期:2000-12-01 00:00:00
abstract::The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prop...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1999.83124.x
更新日期:1999-08-01 00:00:00
abstract::Nuclear ribosomal ITS2 and mitochondrial cytochrome oxidase DNA sequences were analysed from the Byturidae (Coleoptera), which includes the raspberry beetles. The secondary structure of ITS2 was plotted and interspecific changes analysed. Evidence for selection on simple sequence repeats within the ITS2 was found. Phy...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00266.x
更新日期:2001-06-01 00:00:00
abstract::Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12315
更新日期:2017-10-01 00:00:00
abstract::The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1997.00151.x
更新日期:1997-02-01 00:00:00
abstract::The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12130
更新日期:2014-12-01 00:00:00
abstract::In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12590
更新日期:2019-12-01 00:00:00
abstract::We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2005.00585.x
更新日期:2005-10-01 00:00:00
abstract::Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00765.x
更新日期:2007-12-01 00:00:00
abstract::Insects and other arthropods have an effective innate immune system that can clear infections with bacteria and other microorganisms. Despite this ability, one group of bacteria, the spiroplasmas, survive unharmed within the haemolymph of a wide range of arthropod hosts. We investigated the interaction between one mem...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00380.x
更新日期:2003-02-01 00:00:00
abstract::To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2009.00911.x
更新日期:2009-11-01 00:00:00