Vitellogenin gene expression in autogenous Culex tarsalis.

abstract：:We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...

journal_title：Insect molecular biology

authors： Chang WX,Gahan LJ,Tabashnik BE,Heckel DG

更新日期：1999-05-01 00:00:00

abstract：:Insects and other arthropods have an effective innate immune system that can clear infections with bacteria and other microorganisms. Despite this ability, one group of bacteria, the spiroplasmas, survive unharmed within the haemolymph of a wide range of arthropod hosts. We investigated the interaction between one mem...

journal_title：Insect molecular biology

authors： Hurst GD,Anbutsu H,Kutsukake M,Fukatsu T

更新日期：2003-02-01 00:00:00

abstract：:The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST of Bombyx mori (bmGSTO) was shown to be induced after exposure to various environmental stresses. A soluble form of recombinant protein (rbmGSTO) was functionally ...

journal_title：Insect molecular biology

authors： Yamamoto K,Teshiba S,Shigeoka Y,Aso Y,Banno Y,Fujiki T,Katakura Y

更新日期：2011-06-01 00:00:00

abstract：:Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...

journal_title：Insect molecular biology

authors： Quan GX,Kanda T,Tamura T

更新日期：2002-06-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00

abstract：:Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...

journal_title：Insect molecular biology

authors： Sun ZC,Wu M,Miller TA,Han ZJ

更新日期：2008-02-01 00:00:00

abstract：:The brown planthopper (BPH), Nilaparvata lugens, is a major threat to rice production. The eggshell plays an important role in insect reproduction. The constituents and formation process of BPH eggshells remains largely unknown. Here, we report a novel eggshell-associated protein, NlChP38, containing an amelogenin dom...

journal_title：Insect molecular biology

authors： Lou YH,Lu JB,Li DT,Ye YX,Luo XM,Zhang CX

更新日期：2019-10-01 00:00:00

abstract：:Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases (CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The...

journal_title：Insect molecular biology

authors： Yang ZM,Yu N,Wang SJ,Korai SK,Liu ZW

更新日期：2021-02-01 00:00:00

abstract：:The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...

journal_title：Insect molecular biology

authors： Campbell BC,Steffen-Campbell JD,Werren JH

更新日期：1993-01-01 00:00:00

abstract：:Nuclear ribosomal ITS2 and mitochondrial cytochrome oxidase DNA sequences were analysed from the Byturidae (Coleoptera), which includes the raspberry beetles. The secondary structure of ITS2 was plotted and interspecific changes analysed. Evidence for selection on simple sequence repeats within the ITS2 was found. Phy...

journal_title：Insect molecular biology

authors： Malloch G,Fenton B,Goodrich MA

更新日期：2001-06-01 00:00:00

abstract：:Haemocyanins constitute a group of copper-containing respiratory proteins, and hexamerins were derived from hexapod haemocyanin but lost the ability to transport oxygen and serve as storage proteins. Although hexamerins have been reported in most insect species, none of them has been identified in Collembola, one of t...

journal_title：Insect molecular biology

authors： Liang Y,Xie W,Luan YX

更新日期：2019-10-01 00:00:00

abstract：:In mammals, the mitogen-activated protein (MAP) kinase pathway is one of the four major signalling systems that respond to stress and inflammatory stimuli. A full-length cDNA corresponding to Aedes aegypti MAP kinase kinase 3 (AaMEK3) was cloned and sequenced. It is 1.7 kb and contains an open reading frame of 334 ami...

journal_title：Insect molecular biology

authors： Huang SJ,Wu RC,Shaio MF,Wang PS,Cho WL

更新日期：2003-12-01 00:00:00

abstract：:Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associ...

journal_title：Insect molecular biology

authors： Ranson H,Paton MG,Jensen B,McCarroll L,Vaughan A,Hogan JR,Hemingway J,Collins FH

更新日期：2004-08-01 00:00:00

abstract：:The muga silkworm, Antheraea assamensis, is exclusively present in the northeastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture-dependent techniques, generic identification using 16S ribosomal RNA probes, diversity analysis and qualitative screening f...

journal_title：Insect molecular biology

authors： Gandotra S,Kumar A,Naga K,Bhuyan PM,Gogoi DK,Sharma K,Subramanian S

更新日期：2018-10-01 00:00:00

abstract：:Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...

journal_title：Insect molecular biology

authors： Hens K,Lemey P,Macours N,Francis C,Huybrechts R

更新日期：2004-12-01 00:00:00

abstract：:Maxadilan is an approximately 7kDa peptide that occurs in the saliva of the sand fly Lutzomyia longipalpis. This peptide is a potent vasodilator and may also have immunomodulatory effects related to the pathogenesis of leishmanial infections. Variation in the primary DNA and inferred amino acid sequence of maxadilan i...

journal_title：Insect molecular biology

authors： Lanzaro GC,Lopes AH,Ribeiro JM,Shoemaker CB,Warburg A,Soares M,Titus RG

更新日期：1999-05-01 00:00:00

abstract：:In this paper we describe the peculiar structures and preferential codon usage found in wild silkworm fibroin genes. We determined a 1350 bp nucleotide sequence from the Chinese oak silkworm, Antheraea pernyi. The deduced amino acid sequence was partitioned into thirteen polyalanine-containing repetitive motifs, which...

journal_title：Insect molecular biology

authors： Yukuhiro K,Kanda T,Tamura T

更新日期：1997-02-01 00:00:00

abstract：:Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...

journal_title：Insect molecular biology

authors： Merlin C,Rosell G,Carot-Sans G,François MC,Bozzolan F,Pelletier J,Jacquin-Joly E,Guerrero A,Maïbèche-Coisne M

更新日期：2007-02-01 00:00:00

abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

journal_title：Insect molecular biology

authors： Biessmann H,Donath J,Walter MF

更新日期：1996-02-01 00:00:00

abstract：:To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...

journal_title：Insect molecular biology

authors： Tadano H,Yamazaki Y,Takeuchi H,Kubo T

更新日期：2009-11-01 00:00:00

abstract：:Insulin-like peptides (ILPs) including insulin, insulin-like growth factor (IGF) and relaxin are evolutionarily conserved hormones in metazoans, and they are involved in diverse physiological processes. The migratory brown planthopper (BPH), Nilaparvata lugens, encodes four ILP genes (Nlilp1, Nlilp2, Nlilp3 and Nlilp4...

journal_title：Insect molecular biology

authors： Xue WH,Liu YL,Jiang YQ,He SF,Wang QQ,Yang ZN,Xu HJ

更新日期：2020-06-01 00:00:00

abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...

journal_title：Insect molecular biology

authors： Vyazunova I,Wessley V,Kim M,Lan Q

更新日期：2007-06-01 00:00:00

abstract：:Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, L...

journal_title：Insect molecular biology

authors： Yang Y,Zhao X,Niu N,Zhao Y,Liu W,Moussian B,Zhang J

更新日期：2020-12-01 00:00:00

abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

journal_title：Insect molecular biology

authors： Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

更新日期：1994-08-01 00:00:00

abstract：:Culex quinquefasciatus is a vector of many diseases that adversely impact human and animal health; however, compared to other mosquito vectors limited genome engineering technologies have been characterized for this vector. Clustered regularly interspaced short palindrome repeats-associated protein 9 (CRISPR-Cas9) bas...

journal_title：Insect molecular biology

authors： Li M,Li T,Liu N,Raban RR,Wang X,Akbari OS

更新日期：2020-04-01 00:00:00

abstract：:In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...

journal_title：Insect molecular biology

authors： Li F,Zhao X,Li M,He K,Huang C,Zhou Y,Li Z,Walters JR

更新日期：2019-12-01 00:00:00

abstract：:Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...

journal_title：Insect molecular biology

authors： Foy BD,Myles KM,Pierro DJ,Sanchez-Vargas I,Uhlírová M,Jindra M,Beaty BJ,Olson KE

更新日期：2004-02-01 00:00:00

abstract：:Juvenile hormone esterase (JHE) activity in the haemolymph of the Colorado potato beetle is necessary to initiate pupation in larvae as well as diapause in adults. The enzyme appears in the haemolymph as a dimer consisting of two 57 kDa subunits. The sequence of an encoding cDNA, JHE.A, is distinct from lepidopteran J...

journal_title：Insect molecular biology

authors： Vermunt AM,Koopmanschap AB,Vlak JM,de Kort CA

更新日期：1998-11-01 00:00:00

abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...

journal_title：Insect molecular biology

authors： Horard B,Mangé A,Pélissier B,Couble P

更新日期：1994-11-01 00:00:00

abstract：:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

journal_title：Insect molecular biology

authors： Wang J,Ren X,Miller TA,Park Y

更新日期：2006-08-01 00:00:00