An immune signalling kinase AaMEK3 from mosquitoes: cDNA cloning and characterization.

Abstract:

:In mammals, the mitogen-activated protein (MAP) kinase pathway is one of the four major signalling systems that respond to stress and inflammatory stimuli. A full-length cDNA corresponding to Aedes aegypti MAP kinase kinase 3 (AaMEK3) was cloned and sequenced. It is 1.7 kb and contains an open reading frame of 334 amino acids and eleven conserved kinase domains, including signatures of a putative serine/threonine kinase active site and an ATP binding site. The messenger (mRNA) and protein expression levels of AaMEK3 are enhanced post bacterial inoculation. The in vitro kinase activity assay reveals that (1) AaMEK3 is not autophosphorylated but can phosphorylate myelin basic protein successfully, and (2) it is slightly enhanced by lipopolysaccharide stimulation. This suggests that AaMEK3 may be involved in mosquito immune signalling.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Huang SJ,Wu RC,Shaio MF,Wang PS,Cho WL

doi

10.1046/j.1365-2583.2003.00444.x

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

595-603

issue

6

eissn

0962-1075

issn

1365-2583

journal_volume

12

pub_type

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