Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina.

Abstract:

:We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expressed in the C-type lectin-producing tissue (CLPT) located at the entrance of the food canal and between the labellum and haustellum. Immunoblot analysis showed that the mature form of CLEM 36 protein was synthesized in the CLPT, then secreted into saliva. Our results indicate that CLEM 36 protein may play an important role in biological defence against pathogens during the food intake of this insect.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Yamamoto-Kihara M,Kotani E

doi

10.1111/j.0962-1075.2004.00468.x

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

133-40

issue

2

eissn

0962-1075

issn

1365-2583

pii

IMB468

journal_volume

13

pub_type

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