Abstract:
:We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expressed in the C-type lectin-producing tissue (CLPT) located at the entrance of the food canal and between the labellum and haustellum. Immunoblot analysis showed that the mature form of CLEM 36 protein was synthesized in the CLPT, then secreted into saliva. Our results indicate that CLEM 36 protein may play an important role in biological defence against pathogens during the food intake of this insect.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Yamamoto-Kihara M,Kotani Edoi
10.1111/j.0962-1075.2004.00468.xsubject
Has Abstractpub_date
2004-04-01 00:00:00pages
133-40issue
2eissn
0962-1075issn
1365-2583pii
IMB468journal_volume
13pub_type
杂志文章abstract::The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1998.00063.x
更新日期:1998-08-01 00:00:00
abstract::Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...
journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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abstract::Haemolymph polypeptides from Plasmodium-refractory and -susceptible mosquitoes were compared by one- and two-dimensional gel electrophoresis. The refractory strain of Anopheles gambiae kills malaria parasites by a humoral melanization mechanism whereas the parasites develop normally in susceptible mosquitoes. The two ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2000.00157.x
更新日期:2000-02-01 00:00:00
abstract::Phenotypic diversity is frequently generated by differences in gene expression. In this study, we addressed the relationship between homology in gene expression and phenotype among four species of eusocial wasps. Specifically, we investigated the evolution of caste-specific and sex-specific gene expression patterns as...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01021.x
更新日期:2010-10-01 00:00:00
abstract::The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1997.00151.x
更新日期:1997-02-01 00:00:00
abstract::RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12301
更新日期:2017-06-01 00:00:00
abstract::Hztransib, recently identified from Helicoverpa zea, represents the first intact and transcriptionally active Transib element. Its open reading frame was detected in Helicoverpa armigera, from which H. zea evolved, and in Helicoverpa assulta, the common ancestor of H. zea and H. armigera, but its remaining parts were ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2010.01061.x
更新日期:2011-06-01 00:00:00
abstract::Horizontal transfer (HT), the exchange of genetic material between species, plays important roles in transposon biology and genome evolution. In this study, we provide the first documented example of a new Academ transposon involved in recent and distant HTs into the genomes of species belonging to seven different ord...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12225
更新日期:2016-06-01 00:00:00
abstract::Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2008.00856.x
更新日期:2009-02-01 00:00:00
abstract::We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other specie...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1993.tb00094.x
更新日期:1993-01-01 00:00:00
abstract::Lepidoptera is one of the most widespread insect orders and includes several agriculturally important insect species. Ecological success of the lepidopteran insects partly depends on their adaptive chemoreception tactics, which play an important role in the selection of hosts, egg-laying sites and mates. Members of th...
journal_title:Insect molecular biology
pub_type: 杂志文章,评审
doi:10.1111/imb.12246
更新日期:2016-10-01 00:00:00
abstract::Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2004.00464.x
更新日期:2004-02-01 00:00:00
abstract::Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2004.00526.x
更新日期:2005-01-01 00:00:00
abstract::The western corn rootworm (WCR), a major pest of maize, is notorious for rapidly adapting biochemically, behaviourally and developmentally to a variety of control methods. Despite much effort, the genetic basis of WCR adaptation remains a mystery. Since transformation-based applications such as transposon tagging and ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12305
更新日期:2017-08-01 00:00:00
abstract::The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily of pheromone/odourant binding proteins. Although D7 proteins are among the most abundant salivary proteins in adult female mosquitoes and sand flies, their role in blood feeding remains elusive. In the present w...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2002.00319.x
更新日期:2002-04-01 00:00:00
abstract::The maternally inherited bacterium Wolbachia is well known for spreading in natural populations by manipulating the reproduction of its arthropod hosts, but can also have mutualist effects that increase host fitness. In mosquitoes and Drosophila some Wolbachia strains can lead to an increase in survival of virus-infec...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12245
更新日期:2016-10-01 00:00:00
abstract::Olfaction plays an important role in insects in recognizing volatile compounds, which are used to find food and mates, as well as avoid danger, predators and pathogens. The key players in the detection of volatile compounds are olfactory receptor (OR) proteins, which are located within the dendritic membrane of sensor...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12096
更新日期:2014-08-01 00:00:00
abstract::The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Med...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00737.x
更新日期:2007-08-01 00:00:00
abstract::Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2007.00765.x
更新日期:2007-12-01 00:00:00
abstract::Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00643.x
更新日期:2006-06-01 00:00:00
abstract::The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2005.00602.x
更新日期:2005-12-01 00:00:00
abstract::We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference be...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00628.x
更新日期:2006-04-01 00:00:00
abstract::Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2002.00328.x
更新日期:2002-06-01 00:00:00
abstract::The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12117
更新日期:2014-12-01 00:00:00
abstract::Apis mellifera plays crucial roles in maintaining the balance of global ecosystems and stability of agricultural systems by helping pollination of flowering plants, including many crops. In recent years, this balance has been disrupted greatly by some pesticides, which results in great losses of honeybees worldwide. P...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12390
更新日期:2018-08-01 00:00:00
abstract::Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various m...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12500
更新日期:2018-10-01 00:00:00
abstract::Despite the importance of locust density-dependent polyphenism as a model system for understanding phenotypic plasticity, there is still much to be learnt about its underlying molecular control. Here we describe the first investigation into the expression of genes encoding the DNA methylation machinery in the migrator...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12203
更新日期:2016-04-01 00:00:00
abstract::The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12226
更新日期:2016-08-01 00:00:00
abstract::A phylogenetic study, based on maximum parsimony, of ten species in the Anopheles punctulatus group of malaria vectors from the south-west Pacific was performed using structural and similarity-based DNA sequence alignments of the nuclear small ribosomal subunit (SSU = 18S). The structural alignment proved to be more i...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2000.00211.x
更新日期:2000-10-01 00:00:00