Efficacy of RNA interference knockdown using aerosolized short interfering RNAs bound to nanoparticles in three diverse aphid species.

abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

journal_title：Insect molecular biology

authors： Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

更新日期：1994-08-01 00:00:00

abstract：:Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...

journal_title：Insect molecular biology

authors： Sun ZC,Wu M,Miller TA,Han ZJ

更新日期：2008-02-01 00:00:00

abstract：:Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...

journal_title：Insect molecular biology

authors： Altincicek B,Vilcinskas A

更新日期：2009-02-01 00:00:00

abstract：:To begin to explore the molecular dynamics of rickettsial tick interaction, subtractive hybridization was used to screen mRNAs in Rickettsia montanensis-infected and uninfected Dermacentor variabilis. We isolated 30 cDNA fragments, 22 of which were up-regulated and eight were down-regulated in response to rickettsial ...

journal_title：Insect molecular biology

authors： Mulenga A,Macaluso KR,Simser JA,Azad AF

更新日期：2003-04-01 00:00:00

abstract：:Culex pipiens quinquefasciatus Say and Culex pipiens pipiens Linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. The two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic ...

journal_title：Insect molecular biology

authors： Sanogo YO,Dobson SL,Bordenstein SR,Novak RJ

更新日期：2007-04-01 00:00:00

abstract：:We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...

journal_title：Insect molecular biology

authors： Sinclair BJ,Gibbs AG,Roberts SP

更新日期：2007-08-01 00:00:00

abstract：:Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...

journal_title：Insect molecular biology

authors： Tsubota T,Minakuchi C,Nakakura T,Shinoda T,Shiotsuki T

更新日期：2010-08-01 00:00:00

abstract：:Apis mellifera plays crucial roles in maintaining the balance of global ecosystems and stability of agricultural systems by helping pollination of flowering plants, including many crops. In recent years, this balance has been disrupted greatly by some pesticides, which results in great losses of honeybees worldwide. P...

journal_title：Insect molecular biology

authors： Hu YT,Tang CK,Wu CP,Wu PC,Yang EC,Tai CC,Wu YL

更新日期：2018-08-01 00:00:00

abstract：:Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...

journal_title：Insect molecular biology

authors： Russell CL,Heesom KJ,Arthur CJ,Helps CR,Mellor PS,Day MJ,Torsteinsdottir S,Björnsdóttir TS,Wilson AD

更新日期：2009-06-01 00:00:00

abstract：:Fast and reliable methods for assessing the mobility of the transposable element Minos have been developed. These methods are based on the detection of excision and insertion of Minos transposons from and into plasmids which are co-introduced into cells. Excision is detected by polymerase chain reaction (PCR) with app...

journal_title：Insect molecular biology

authors： Klinakis AG,Loukeris TG,Pavlopoulos A,Savakis C

更新日期：2000-06-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...

journal_title：Insect molecular biology

authors： Nagoshi RN,Meagher R

更新日期：2003-10-01 00:00:00

abstract：:A gene from the Mediterranean fruit fly (Medfly) Ceratitis capitata, belonging to the hsp70 family of genes, has been cloned and characterized. This gene appears to be an example of a heat shock cognate (hsc) gene based on a variety of structural and functional criteria. The heat shock cognate genes serve critical rol...

journal_title：Insect molecular biology

authors： Thanaphum S,Haymer DS

更新日期：1998-02-01 00:00:00

abstract：:A transferrin (Tf) gene has been predicted from an expressed sequence tag of the diamondback moth, Plutella xylostella. It encodes 681 amino acid residues that share 80-90% sequence homologies with other lepidopteran Tfs. The gene was constitutively expressed in all developmental stages of P. xylostella. Double-strand...

journal_title：Insect molecular biology

authors： Kim J,Kim Y

更新日期：2010-08-01 00:00:00

abstract：:Olfaction plays an important role in insects in recognizing volatile compounds, which are used to find food and mates, as well as avoid danger, predators and pathogens. The key players in the detection of volatile compounds are olfactory receptor (OR) proteins, which are located within the dendritic membrane of sensor...

journal_title：Insect molecular biology

authors： Liu C,Liu Y,Guo M,Cao D,Dong S,Wang G

更新日期：2014-08-01 00:00:00

abstract：:We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...

journal_title：Insect molecular biology

authors： Wang J,Staten R,Miller TA,Park Y

更新日期：2005-10-01 00:00:00

abstract：:PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...

journal_title：Insect molecular biology

authors： Thompson GJ,Crozier YC,Crozier RH

更新日期：2003-02-01 00:00:00

abstract：:A novel receptor guanylyl cyclase (GC) has been identified from the oriental fruit fly Bactrocera dorsalis (Hendel) and has been designated BdmGC-1. Protein domain analysis revealed that BdmGC-1 possesses a characteristic domain organization similar to all known receptor GCs but with a unique carboxyl-terminal extensi...

journal_title：Insect molecular biology

authors： Chang JC,Yang RB,Chen YH,Lu KH

更新日期：2006-02-01 00:00:00

abstract：:Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

journal_title：Insect molecular biology

authors： Zandawala M,Hamoudi Z,Lange AB,Orchard I

更新日期：2015-04-01 00:00:00

abstract：:Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...

journal_title：Insect molecular biology

authors： Lin Y,Liu H,Yang C,Gu J,Shen G,Zhang H,Chen E,Han C,Zhang Y,Xu Y,Wu J,Xia Q

更新日期：2017-10-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thu...

journal_title：Insect molecular biology

authors： Sun ZJ,Li ZX

更新日期：2018-12-01 00:00:00

abstract：:The pea aphid (Acyrthosiphon pisum) is the first whole genome sequenced insect with a hemimetabolic development and an emerging model organism for studies in ecology, evolution and development. The insect steroid moulting hormone 20-hydroxyecdysone (20E) controls and coordinates development in insects, especially the ...

journal_title：Insect molecular biology

authors： Christiaens O,Iga M,Velarde RA,Rougé P,Smagghe G

更新日期：2010-03-01 00:00:00

abstract：:Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...

journal_title：Insect molecular biology

authors： Collins AM,Caperna TJ,Williams V,Garrett WM,Evans JD

更新日期：2006-10-01 00:00:00

abstract：:The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...

journal_title：Insect molecular biology

authors： Jordan TV,Shike H,Boulo V,Cedeno V,Fang Q,Davis BS,Jacobs-Lorena M,Higgs S,Fryxell KJ,Burns JC

更新日期：1998-08-01 00:00:00

abstract：:The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilized for insect germline transformation. The described ...

journal_title：Insect molecular biology

authors： Gregory M,Alphey L,Morrison NI,Shimeld SM

更新日期：2016-06-01 00:00:00

abstract：:The expression pattern of the 79B actin gene in Drosophila melanogaster has been inferred previously by means of a reporter gene in which 79B actin promoter sequences drive the lacZ coding sequences. Although the 79B actin gene is expressed primarily in muscles of the thorax and first abdominal segment of the adults o...

journal_title：Insect molecular biology

authors： Ohshima S,Villarimo C,Gailey DA

更新日期：1997-08-01 00:00:00

abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

journal_title：Insect molecular biology

authors： Biessmann H,Donath J,Walter MF

更新日期：1996-02-01 00:00:00

abstract：:The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST of Bombyx mori (bmGSTO) was shown to be induced after exposure to various environmental stresses. A soluble form of recombinant protein (rbmGSTO) was functionally ...

journal_title：Insect molecular biology

authors： Yamamoto K,Teshiba S,Shigeoka Y,Aso Y,Banno Y,Fujiki T,Katakura Y

更新日期：2011-06-01 00:00:00

abstract：:A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila me...

journal_title：Insect molecular biology

authors： Reiss RA,James AA

更新日期：1993-01-01 00:00:00

abstract：:Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicator...

journal_title：Insect molecular biology

authors： Thibault ST,Luu HT,Vann N,Miller TA

更新日期：1999-02-01 00:00:00