Abstract：
:Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against three databases, general species, honey bees and fruit flies, were performed. Spectra were used to query the recently generated honey bee genome protein list as well as general species and fruit fly databases. Of the 69 unique honey bee proteins found, 66 are also in Drosophila melanogaster. Two proteins only matched honey bee genes and one is a widespread protein lost from the fly genome. There is over-representation of genes implicated in the glycolysis pathway. Metabolism-associated proteins were found primarily in the seminal vesicle. Male accessory gland proteins as identified in Drosophila rarely had orthologs among proteins found in the honey bee. A complete listing of gel spots chosen including honey bee genome matches and Mascot searches of MALDI-TOF results with statistics is in the Supplementary table. MALDI-TOF spectra and more complete Mascot peptide mass fingerprinting data are available on request. Supplementary figs 1-3 show the stained protein gels.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Collins AM,Caperna TJ,Williams V,Garrett WM,Evans JDdoi
10.1111/j.1365-2583.2006.00674.xsubject
Has Abstractpub_date
2006-10-01 00:00:00pages
541-9issue
5eissn
0962-1075issn
1365-2583pii
IMB674journal_volume
15pub_type
杂志文章abstract：:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2003.00425.x
更新日期：2003-08-01 00:00:00
abstract：:We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference be...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2006.00628.x
更新日期：2006-04-01 00:00:00
abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00175.x
更新日期：1994-11-01 00:00:00
abstract：:Olfaction plays an important role in insects in recognizing volatile compounds, which are used to find food and mates, as well as avoid danger, predators and pathogens. The key players in the detection of volatile compounds are olfactory receptor (OR) proteins, which are located within the dendritic membrane of sensor...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12096
更新日期：2014-08-01 00:00:00
abstract：:A phylogenetic study, based on maximum parsimony, of ten species in the Anopheles punctulatus group of malaria vectors from the south-west Pacific was performed using structural and similarity-based DNA sequence alignments of the nuclear small ribosomal subunit (SSU = 18S). The structural alignment proved to be more i...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00211.x
更新日期：2000-10-01 00:00:00
abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2008.00798.x
更新日期：2008-06-01 00:00:00
abstract：:Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2002.00328.x
更新日期：2002-06-01 00:00:00
abstract：:A gene from the Mediterranean fruit fly (Medfly) Ceratitis capitata, belonging to the hsp70 family of genes, has been cloned and characterized. This gene appears to be an example of a heat shock cognate (hsc) gene based on a variety of structural and functional criteria. The heat shock cognate genes serve critical rol...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1998.71051.x
更新日期：1998-02-01 00:00:00
abstract：:A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00567.x
更新日期：2005-08-01 00:00:00
abstract：:Culex pipiens quinquefasciatus Say and Culex pipiens pipiens Linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. The two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2006.00707.x
更新日期：2007-04-01 00:00:00
abstract：:A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2001.00249.x
更新日期：2001-04-01 00:00:00
abstract：:Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12058
更新日期：2014-02-01 00:00:00
abstract：:In mammals, the mitogen-activated protein (MAP) kinase pathway is one of the four major signalling systems that respond to stress and inflammatory stimuli. A full-length cDNA corresponding to Aedes aegypti MAP kinase kinase 3 (AaMEK3) was cloned and sequenced. It is 1.7 kb and contains an open reading frame of 334 ami...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2003.00444.x
更新日期：2003-12-01 00:00:00
abstract：:Insect chitinases are hydrolytic enzymes required for the degradation of chitin. They are essential for insect moulting and metamorphosis. In this study, the regulation mechanism of a chitinase gene, Bombyx mori chitinase 5 (BmCHT5), was studied. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that Bm...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12288
更新日期：2017-04-01 00:00:00
abstract：:PCR primers designed from the alpha-esterase gene cluster of Drosophila melanogaster have been used to isolate fragments from eight esterase genes in the Australian sheep blowfly, Lucilia cuprina. Phylogenetic analysis suggests that three are homologues of the alpha E7, alpha E8 and alpha E9 genes of the alpha-esteras...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1996.tb00056.x
更新日期：1996-08-01 00:00:00
abstract：:ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Droso...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.0962-1075.2004.00512.x
更新日期：2004-10-01 00:00:00
abstract：:Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect m...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1995.tb00028.x
更新日期：1995-11-01 00:00:00
abstract：:Phenotypic diversity is frequently generated by differences in gene expression. In this study, we addressed the relationship between homology in gene expression and phenotype among four species of eusocial wasps. Specifically, we investigated the evolution of caste-specific and sex-specific gene expression patterns as...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.01021.x
更新日期：2010-10-01 00:00:00
abstract：:Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional t...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00275.x
更新日期：2001-08-01 00:00:00
abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1996.tb00035.x
更新日期：1996-02-01 00:00:00
abstract：:Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various m...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12500
更新日期：2018-10-01 00:00:00
abstract：:Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00561.x
更新日期：2005-06-01 00:00:00
abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00165.x
更新日期：1994-08-01 00:00:00
abstract：:Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2012.01145.x
更新日期：2012-08-01 00:00:00
abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2007.00729.x
更新日期：2007-06-01 00:00:00
abstract：:The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1998.00063.x
更新日期：1998-08-01 00:00:00
abstract：:We recently showed that the Masculinizer gene (Masc) plays a primary role in sex determination in the lepidopteran model insect Bombyx mori. However, it remains unknown whether this Masc protein-dependent sex determination system is conserved amongst lepidopteran insects or within the family Bombycidae. Here we cloned...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12181
更新日期：2015-10-01 00:00:00
abstract：:Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00905.x
更新日期：2009-10-01 00:00:00
abstract：:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00917.x
更新日期：2009-11-01 00:00:00
abstract：:E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12244
更新日期：2016-10-01 00:00:00
