Abstract:
:We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference between PCNA proteins from Ae. albopictus and Ae. aegypti. In An. gambiae, the PCNA homolog contained 260 residues, and the pcna gene was interrupted by a single 67 nucleotide intron in the betaC2 region of the protein. A phylogenetic comparison grouped known Dipteran PCNA sequences into two clusters, representing the Nematocera and the Cyclorrhapha. PCNA transcripts measured 1.1 kb, and were stable, as was PCNA protein. Mosquito PCNA was efficiently recognized by a commercially available mouse anti-PCNA monoclonal antibody, which coprecipitated 29 kDa and 35 kDa proteins from mosquito cells representing different growth states. These results support the feasibility of recovering mosquito cell cycle inhibitory proteins by virtue of their interaction with PCNA.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Ma L,Gerenday A,Coley KM,Fallon AMdoi
10.1111/j.1365-2583.2006.00628.xsubject
Has Abstractpub_date
2006-04-01 00:00:00pages
197-205issue
2eissn
0962-1075issn
1365-2583pii
IMB628journal_volume
15pub_type
杂志文章abstract::PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...
journal_title:Insect molecular biology
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.1998.71051.x
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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更新日期:2007-06-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
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abstract::Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...
journal_title:Insect molecular biology
pub_type: 杂志文章
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abstract::We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...
journal_title:Insect molecular biology
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