Co-immunoprecipitation of putative proteins that interact with mosquito proliferating cell nuclear antigen.

Abstract:

:We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference between PCNA proteins from Ae. albopictus and Ae. aegypti. In An. gambiae, the PCNA homolog contained 260 residues, and the pcna gene was interrupted by a single 67 nucleotide intron in the betaC2 region of the protein. A phylogenetic comparison grouped known Dipteran PCNA sequences into two clusters, representing the Nematocera and the Cyclorrhapha. PCNA transcripts measured 1.1 kb, and were stable, as was PCNA protein. Mosquito PCNA was efficiently recognized by a commercially available mouse anti-PCNA monoclonal antibody, which coprecipitated 29 kDa and 35 kDa proteins from mosquito cells representing different growth states. These results support the feasibility of recovering mosquito cell cycle inhibitory proteins by virtue of their interaction with PCNA.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Ma L,Gerenday A,Coley KM,Fallon AM

doi

10.1111/j.1365-2583.2006.00628.x

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

197-205

issue

2

eissn

0962-1075

issn

1365-2583

pii

IMB628

journal_volume

15

pub_type

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