Evidence for two juvenile hormone esterase-related genes in the Colorado potato beetle.

Abstract:

:Juvenile hormone esterase (JHE) activity in the haemolymph of the Colorado potato beetle is necessary to initiate pupation in larvae as well as diapause in adults. The enzyme appears in the haemolymph as a dimer consisting of two 57 kDa subunits. The sequence of an encoding cDNA, JHE.A, is distinct from lepidopteran JHEs. In this study, RT-PCR using primers designed on the basis of the 5'- and 3'-ends of the coding region revealed the existence of a related gene, JHE.B. The presence of two JHE-related genes was also shown by PCR amplification on genomic DNA from different individual beetles followed by restriction enzyme analysis. Both forms, probably paralogues, were transcribed since they could be amplified on messenger RNA from fat bodies. The size of the PCR products generated with mRNA and genomic DNA were both 1.6 kb, suggesting the absence of introns in the genomic JHE coding sequence. The sequence of a genomic clone, which encoded JHE.B, was 77% identical and 82% similar in amino acids compared to JHE.A. No introns were found in the coding sequence of these coleopteran JHE-related genes, in contrast to lepidopteran JHE genes. Southern blot analysis of digested genomic DNA confirmed the presence of two JHE-related genes.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Vermunt AM,Koopmanschap AB,Vlak JM,de Kort CA

doi

10.1046/j.1365-2583.1998.740327.x

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

327-36

issue

4

eissn

0962-1075

issn

1365-2583

journal_volume

7

pub_type

杂志文章
  • Isolation of alpha cluster esterase genes associated with organophosphate resistance in Lucilia cuprina.

    abstract::PCR primers designed from the alpha-esterase gene cluster of Drosophila melanogaster have been used to isolate fragments from eight esterase genes in the Australian sheep blowfly, Lucilia cuprina. Phylogenetic analysis suggests that three are homologues of the alpha E7, alpha E8 and alpha E9 genes of the alpha-esteras...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1996.tb00056.x

    authors: Newcomb RD,East PD,Russell RJ,Oakeshott JG

    更新日期:1996-08-01 00:00:00

  • Molecular characterization of N-methyl-d-aspartate receptor from Bemisia tabaci.

    abstract::The N-methyl-d-aspartate receptors (NMDARs) are ionotropic ligand gated channels that are highly permeable to calcium ions. In insects, NMDARs are associated with glutamatergic neurotransmission governing diverse physiological and biological processes like vitellogenesis and ovarian development. Therefore, NMDAR may a...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12690

    authors: Dixit S,Thakur N,Shukla A,Upadhyay SK,C Verma P

    更新日期:2020-12-27 00:00:00

  • Adipokinetic hormone signalling system in the Chagas disease vector, Rhodnius prolixus.

    abstract::Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12157

    authors: Zandawala M,Hamoudi Z,Lange AB,Orchard I

    更新日期:2015-04-01 00:00:00

  • Narrow tuning of an odorant receptor to plant volatiles in Spodoptera exigua (Hübner).

    abstract::Olfaction plays an important role in insects in recognizing volatile compounds, which are used to find food and mates, as well as avoid danger, predators and pathogens. The key players in the detection of volatile compounds are olfactory receptor (OR) proteins, which are located within the dendritic membrane of sensor...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12096

    authors: Liu C,Liu Y,Guo M,Cao D,Dong S,Wang G

    更新日期:2014-08-01 00:00:00

  • Tehao functions in the Toll pathway in Drosophila melanogaster: possible roles in development and innate immunity.

    abstract::Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.0962-1075.2001.00284.x

    authors: Luo C,Shen B,Manley JL,Zheng L

    更新日期:2001-10-01 00:00:00

  • A glutathione S-transferase gene of the vector mosquito, Anopheles gambiae.

    abstract::A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila me...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1993.tb00122.x

    authors: Reiss RA,James AA

    更新日期:1993-01-01 00:00:00

  • Molecular characterization of insulin-like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).

    abstract::Insulin-like peptides (ILPs) including insulin, insulin-like growth factor (IGF) and relaxin are evolutionarily conserved hormones in metazoans, and they are involved in diverse physiological processes. The migratory brown planthopper (BPH), Nilaparvata lugens, encodes four ILP genes (Nlilp1, Nlilp2, Nlilp3 and Nlilp4...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12636

    authors: Xue WH,Liu YL,Jiang YQ,He SF,Wang QQ,Yang ZN,Xu HJ

    更新日期:2020-06-01 00:00:00

  • piggyBac-like elements in the tobacco budworm, Heliothis virescens (Fabricius).

    abstract::We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00653.x

    authors: Wang J,Ren X,Miller TA,Park Y

    更新日期:2006-08-01 00:00:00

  • The genome of the Queensland fruit fly Bactrocera tryoni contains multiple representatives of the mariner family of transposable elements.

    abstract::Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.0962-1075.2001.00275.x

    authors: Green CL,Frommer M

    更新日期:2001-08-01 00:00:00

  • Expression patterns and colocalization of two sensory neurone membrane proteins in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones.

    abstract::Sensory neurone membrane proteins (SNMPs) function as essential cofactors for insect sex pheromone detection. In this study, we report two SNMPs in Ectropis obliqua Prout, a serious geometrid pest that produces typical Type-II sex pheromones. Sequence alignments and phylogenetic analyses showed that EoblSNMP1 and Eobl...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12555

    authors: Sun L,Wang Q,Zhang Y,Yan Y,Guo H,Xiao Q,Zhang Y

    更新日期:2019-06-01 00:00:00

  • Inducible- and constitutive-type transcript variants of kakusei , a novel non-coding immediate early gene, in the honeybee brain.

    abstract::We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00821.x

    authors: Kiya T,Kunieda T,Kubo T

    更新日期:2008-09-01 00:00:00

  • Morphological characterization and molecular mapping of an irradiation-induced Speckled mutant in the silkworm, Bombyx mori.

    abstract::Speckled (Spc), an X-ray-induced lethal mutant of Bombyx mori, exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in females. Here, we report the morphological characterization and molecular mapping of the Spc mutant. Morphological investigations revealed that the epiderma...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12205

    authors: Tan D,Tong XL,Hu H,Wu SY,Li CL,Xiong G,Xiang ZH,Dai FY,Lu C

    更新日期:2016-04-01 00:00:00

  • Insect genomes: progress and challenges.

    abstract::In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...

    journal_title:Insect molecular biology

    pub_type: 杂志文章,评审

    doi:10.1111/imb.12599

    authors: Li F,Zhao X,Li M,He K,Huang C,Zhou Y,Li Z,Walters JR

    更新日期:2019-12-01 00:00:00

  • Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori.

    abstract::E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12244

    authors: Tanaka H,Sagisaka A,Suzuki N,Yamakawa M

    更新日期:2016-10-01 00:00:00

  • A role for alcohol dehydrogenase in the Drosophila immune response?

    abstract::In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00543.x

    authors: Schlenke TA,McKean KA

    更新日期:2005-04-01 00:00:00

  • Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti.

    abstract::Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00905.x

    authors: Traver BE,Anderson MA,Adelman ZN

    更新日期:2009-10-01 00:00:00

  • Genes of the antioxidant system of the honey bee: annotation and phylogeny.

    abstract::Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00695.x

    authors: Corona M,Robinson GE

    更新日期:2006-10-01 00:00:00

  • An expansion of the genome size dataset for the insect order Hymenoptera, with a first test of parasitism and eusociality as possible constraints.

    abstract::Although the Hymenoptera represent a remarkably diverse and socioeconomically important group that is of considerable interest in genome biology, they remain understudied in terms of genome size. This study reports new genome size estimates for 89 species of ants, bees and wasps, representing 17 families and four supe...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.00992.x

    authors: Ardila-Garcia AM,Umphrey GJ,Gregory TR

    更新日期:2010-06-01 00:00:00

  • Cytochrome P450s CYP6D3 and CYP6D1 are part of a P450 gene cluster on autosome 1 in the house fly.

    abstract::The P450 monooxygenases of insects are important in the metabolism of numerous endogenous and exogenous compounds. However, identity of the P450 isoform(s) involved in these reactions is rarely known. A critical first step in the identification of important P450s is the cloning and sequencing of their genes. Toward th...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2001.00256.x

    authors: Kasai S,Scott JG

    更新日期:2001-06-01 00:00:00

  • Functional characterization of Musca glutamate- and GABA-gated chloride channels expressed independently and coexpressed in Xenopus oocytes.

    abstract::Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00680.x

    authors: Eguchi Y,Ihara M,Ochi E,Shibata Y,Matsuda K,Fushiki S,Sugama H,Hamasaki Y,Niwa H,Wada M,Ozoe F,Ozoe Y

    更新日期:2006-12-01 00:00:00

  • Downregulation of dystrophin expression in pupae of the whitefly Bemisia tabaci inhibits the emergence of adults.

    abstract::The whitefly Bemisia tabaci is a major pest to agriculture. Adults are able to fly for long distances and to colonize staple crops, herbs and ornamentals, and to vector viruses belonging to several important taxonomic groups. During their early development, whiteflies mature from eggs through several nymphal stages (i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12579

    authors: Bar L,Czosnek H,Sobol I,Ghanim M,Hariton Shalev A

    更新日期:2019-10-01 00:00:00

  • 20-hydroxyecdysone enhances the expression of the chitinase 5 via Broad-Complex Zinc-Finger 4 during metamorphosis in silkworm, Bombyx mori.

    abstract::Insect chitinases are hydrolytic enzymes required for the degradation of chitin. They are essential for insect moulting and metamorphosis. In this study, the regulation mechanism of a chitinase gene, Bombyx mori chitinase 5 (BmCHT5), was studied. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that Bm...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12288

    authors: Zhang X,Zheng S

    更新日期:2017-04-01 00:00:00

  • RNA interference in mosquito: understanding immune responses, double-stranded RNA delivery systems and potential applications in vector control.

    abstract::RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章,评审

    doi:10.1111/imb.12282

    authors: Balakrishna Pillai A,Nagarajan U,Mitra A,Krishnan U,Rajendran S,Hoti SL,Mishra RK

    更新日期:2017-04-01 00:00:00

  • Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes.

    abstract::Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2012.01141.x

    authors: Adelman ZN,Anderson MA,Liu M,Zhang L,Myles KM

    更新日期:2012-06-01 00:00:00

  • Fall armyworm FR sequences map to sex chromosomes and their distribution in the wild indicate limitations in interstrain mating.

    abstract::The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00429.x

    authors: Nagoshi RN,Meagher R

    更新日期:2003-10-01 00:00:00

  • Evidence for horizontal transfer of a recently active Academ transposon.

    abstract::Horizontal transfer (HT), the exchange of genetic material between species, plays important roles in transposon biology and genome evolution. In this study, we provide the first documented example of a new Academ transposon involved in recent and distant HTs into the genomes of species belonging to seven different ord...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12225

    authors: Zhang HH,Shen YH,Xiong XM,Han MJ,Qi DW,Zhang XG

    更新日期:2016-06-01 00:00:00

  • The function of spineless in antenna and wing development of the brown planthopper, Nilaparvata lugens.

    abstract::A wide array of sensilla are distributed on insect antennae, and they play a variety of important roles. Rice planthoppers, destructive pests on rice, have a unique antenna sensilla structure called the 'sensory plaque organ'. The spineless (ss) gene encodes a bHLH-PAS transcription factor and plays a key role in ante...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12538

    authors: Li X,Liu FZ,Cai WL,Zhao J,Hua HX,Zou YL

    更新日期:2019-04-01 00:00:00

  • The cis-regulatory sequences required for expression of the Drosophila melanogaster adult cuticle gene ACP65A.

    abstract::Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormon...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2009.00893.x

    authors: Lestradet M,Gervasio E,Fraichard S,Dupas S,Alabouvette J,Lemoine A,Charles JP

    更新日期:2009-08-01 00:00:00

  • Efficacy of RNA interference knockdown using aerosolized short interfering RNAs bound to nanoparticles in three diverse aphid species.

    abstract::RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12301

    authors: Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

    更新日期:2017-06-01 00:00:00

  • Silencing chitin deacetylase 2 impairs larval-pupal and pupal-adult molts in Leptinotarsa decemlineata.

    abstract::Insect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N-deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinot...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12524

    authors: Wu JJ,Chen ZC,Wang YW,Fu KY,Guo WC,Li GQ

    更新日期:2019-02-01 00:00:00