piggyBac-like elements in the tobacco budworm, Heliothis virescens (Fabricius).

Abstract:

:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, whereas other HvPLE1 copies and all the HvPLE2 copies carried disruptive mutations in the region encoding transposase. We also identified none to two bands per genome hybridized to a probe of Trichoplusia ni piggyBac in genomic Southern blotting, which are different from HvPLE1 or HvPLE2. Analysis of the sequences of multiple copies of HvPLE1 and HvPLE2 suggests that the PLEs are closely related to the T. ni piggyBac, of relatively young age, and independently entered the H. virescens genome.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Wang J,Ren X,Miller TA,Park Y

doi

10.1111/j.1365-2583.2006.00653.x

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

435-43

issue

4

eissn

0962-1075

issn

1365-2583

pii

IMB653

journal_volume

15

pub_type

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