Abstract：
:Insect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N-deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinotarsa decemlineata. Both splices were abundantly expressed in larval foregut, rectum, and epidermis; their levels peaked immediately before ecdysis within each instar. In vivo results revealed that the two isoforms transcriptionally responded, positively and negatively respectively, to 20-hydroxyecdysone and juvenile hormone signaling pathways. RNA interference (RNAi)-aided knockdown of the two LdCDA2 variants (hereafter LdCDA2) or LdCDA2b, rather than LdCDA2a, resulted in three negative effects. First, foliage consumption was significantly reduced, larval developing period was lengthened, and larval growth was retarded. Second, chitin contents were reduced, whereas glucose, trehalose, and glycogen contents were increased in the LdCDA2 and LdCDA2b RNAi larvae. Third, approximately 20% of LdCDA2 and LdCDA2b RNAi larvae were trapped within the exuviae and finally died. About 60% of the abnormal pupae died as pharate adults. Around 20% of the RNAi pupae emerged as deformed adults, with small size and wrinkled wings. These adults eventually died within 1 week after molting. Our results reveal that knockdown of CDA2 affects chitin accumulation. Consequently, LdCDA2 may be a potential target for control of L. decemlineata larvae.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Wu JJ,Chen ZC,Wang YW,Fu KY,Guo WC,Li GQdoi
10.1111/imb.12524subject
Has Abstractpub_date
2019-02-01 00:00:00pages
52-64issue
1eissn
0962-1075issn
1365-2583journal_volume
28pub_type
杂志文章abstract：:The whitefly Bemisia tabaci is a major pest to agriculture. Adults are able to fly for long distances and to colonize staple crops, herbs and ornamentals, and to vector viruses belonging to several important taxonomic groups. During their early development, whiteflies mature from eggs through several nymphal stages (i...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12579
更新日期：2019-10-01 00:00:00
abstract：:Phosphofructokinase (PFK) is a rate-limiting enzyme in glycolysis, but its linkage with locomotion in termites is not well understood, despite the demonstrated involvement of this gene in the locomotion of different animals. Here, we investigated the effect of the pfk gene on locomotion in the subterranean termite Ret...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12672
更新日期：2021-02-01 00:00:00
abstract：:Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00284.x
更新日期：2001-10-01 00:00:00
abstract：:Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12315
更新日期：2017-10-01 00:00:00
abstract：:The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Med...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2007.00737.x
更新日期：2007-08-01 00:00:00
abstract：:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2006.00643.x
更新日期：2006-06-01 00:00:00
abstract：:The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1998.00063.x
更新日期：1998-08-01 00:00:00
abstract：:A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00567.x
更新日期：2005-08-01 00:00:00
abstract：:A phylogenetic study, based on maximum parsimony, of ten species in the Anopheles punctulatus group of malaria vectors from the south-west Pacific was performed using structural and similarity-based DNA sequence alignments of the nuclear small ribosomal subunit (SSU = 18S). The structural alignment proved to be more i...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00211.x
更新日期：2000-10-01 00:00:00
abstract：:The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12117
更新日期：2014-12-01 00:00:00
abstract：:Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2012.01145.x
更新日期：2012-08-01 00:00:00
abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1996.tb00035.x
更新日期：1996-02-01 00:00:00
abstract：:We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2007.00739.x
更新日期：2007-08-01 00:00:00
abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00936.x
更新日期：2010-02-01 00:00:00
abstract：:The maternally inherited bacterium Wolbachia is well known for spreading in natural populations by manipulating the reproduction of its arthropod hosts, but can also have mutualist effects that increase host fitness. In mosquitoes and Drosophila some Wolbachia strains can lead to an increase in survival of virus-infec...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12245
更新日期：2016-10-01 00:00:00
abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp24D) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 271 amino acid protein that contains the conserved serine, histidine and aspartic acid residues found in serine proteases, and has the highest identity to ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1997.00193.x
更新日期：1997-11-01 00:00:00
abstract：:The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12130
更新日期：2014-12-01 00:00:00
abstract：:In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12590
更新日期：2019-12-01 00:00:00
abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00157.x
更新日期：1994-05-01 00:00:00
abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00602.x
更新日期：2005-12-01 00:00:00
abstract：:A transferrin (Tf) gene has been predicted from an expressed sequence tag of the diamondback moth, Plutella xylostella. It encodes 681 amino acid residues that share 80-90% sequence homologies with other lepidopteran Tfs. The gene was constitutively expressed in all developmental stages of P. xylostella. Double-strand...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.01014.x
更新日期：2010-08-01 00:00:00
abstract：:Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00561.x
更新日期：2005-06-01 00:00:00
abstract：:Abstract Randomly amplified polymorphic DNA (RAPD) analysis was performed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from eighteen locations across eight US states and north-east Mexico. Sixty-seven reproducible bands from six random primers were analysed for genetic va...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.0962-1075.2004.00487.x
更新日期：2004-06-01 00:00:00
abstract：:Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2004.00464.x
更新日期：2004-02-01 00:00:00
abstract：:Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2012.01158.x
更新日期：2012-10-01 00:00:00
abstract：:RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major ...
journal_title：Insect molecular biology
pub_type： 杂志文章,评审
doi：10.1111/imb.12282
更新日期：2017-04-01 00:00:00
abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2008.00798.x
更新日期：2008-06-01 00:00:00
abstract：:In the silk moth Bombyx mori, chorion genes of the same developmental specificity are organized in divergently transcribed α/β gene pairs, sharing a common 5' flanking promoter region. This bidirectional promoter contains a complete set of cis-elements responsible for developmentally accurate gene expression. In the p...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12136
更新日期：2015-02-01 00:00:00
abstract：:The western corn rootworm (WCR), a major pest of maize, is notorious for rapidly adapting biochemically, behaviourally and developmentally to a variety of control methods. Despite much effort, the genetic basis of WCR adaptation remains a mystery. Since transformation-based applications such as transposon tagging and ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12305
更新日期：2017-08-01 00:00:00
abstract：:Ticks continue to be a threat to animal and human health, and new and novel control strategies are needed for ticks and tick-borne pathogens. The characterization of the tick-pathogen interface and the tick immune response to microbial infections is fundamental toward the formulation of new control strategies for tick...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.01037.x
更新日期：2010-10-01 00:00:00
