Abstract:
:In the silk moth Bombyx mori, chorion genes of the same developmental specificity are organized in divergently transcribed α/β gene pairs, sharing a common 5' flanking promoter region. This bidirectional promoter contains a complete set of cis-elements responsible for developmentally accurate gene expression. In the present paper, based on the observation that Bombyx chorion gene promoters contain cis-elements for the same transcription factors without concrete evidence on which of them are essential, we address the question as to how promoter architecture (number, orientation and position of common factor binding sites) facilitates developmentally accurate chorion gene regulation. To this end, we constructed several mutated promoter regions of an early-middle gene pair and cloned them upstream of a reporter gene to introduce these plasmid constructs into silk moth follicle epithelial cells via electroporation as an efficient and quick method for transient expression. This is the first time that an ex vivo method had been applied to test the impact of systematic cis-element mutations on a chorion gene promoter. Our results confirmed the importance of the HMGA factor and the role of the GATA factor as an early repressor, and led to a more detailed understanding of which C/EBP sites participate in the regulation of early-middle chorion gene expression.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Tsatsarounos SP,Rodakis GC,Lecanidou Rdoi
10.1111/imb.12136subject
Has Abstractpub_date
2015-02-01 00:00:00pages
71-81issue
1eissn
0962-1075issn
1365-2583journal_volume
24pub_type
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journal_title:Insect molecular biology
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12301
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journal_title:Insect molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
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