Transfection of cultured cells of the cotton boll weevil, Anthonomus grandis, with a heat-shock-promoter-chloramphenicol-acetyltransferase construct.

abstract：:We recently showed that the Masculinizer gene (Masc) plays a primary role in sex determination in the lepidopteran model insect Bombyx mori. However, it remains unknown whether this Masc protein-dependent sex determination system is conserved amongst lepidopteran insects or within the family Bombycidae. Here we cloned...

journal_title：Insect molecular biology

authors： Lee J,Kiuchi T,Kawamoto M,Shimada T,Katsuma S

更新日期：2015-10-01 00:00:00

abstract：:The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily of pheromone/odourant binding proteins. Although D7 proteins are among the most abundant salivary proteins in adult female mosquitoes and sand flies, their role in blood feeding remains elusive. In the present w...

journal_title：Insect molecular biology

authors： Valenzuela JG,Charlab R,Gonzalez EC,de Miranda-Santos IK,Marinotti O,Francischetti IM,Ribeiro JM

更新日期：2002-04-01 00:00:00

abstract：:Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

journal_title：Insect molecular biology

authors： Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

更新日期：2019-12-01 00:00:00

abstract：:Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional t...

journal_title：Insect molecular biology

authors： Green CL,Frommer M

更新日期：2001-08-01 00:00:00

abstract：:Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, pl...

journal_title：Insect molecular biology

authors： Beard CB,O'Neill SL,Mason P,Mandelco L,Woese CR,Tesh RB,Richards FF,Aksoy S

更新日期：1993-01-01 00:00:00

abstract：:Cyclobutane pyrimidine dimer (CPD) photolyases repair ultraviolet (UV)-induced DNA damage using blue light. To get insight in the origin of baculovirus CPD photolyase (phr) genes, homologues in the lepidopteran insects Chrysodeixis chalcites, Spodoptera exigua and Trichoplusia ni were identified and characterized. Lep...

journal_title：Insect molecular biology

authors： Biernat MA,Ros VI,Vlak JM,van Oers MM

更新日期：2011-08-01 00:00:00

abstract：:The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thu...

journal_title：Insect molecular biology

authors： Sun ZJ,Li ZX

更新日期：2018-12-01 00:00:00

abstract：:Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...

journal_title：Insect molecular biology

authors： Petrova A,Smith CM

更新日期：2014-02-01 00:00:00

abstract：:A birth-and-death evolutionary model for odorant receptor gene repertoires presumes the creation of repertoires with the capacity for high-level diversity and rapid ligand specificity change. This changes the recognised odour space, directly affecting fitness-related behaviours and ultimately affecting adaptation to n...

journal_title：Insect molecular biology

authors： Hill SR,Majeed S,Ignell R

更新日期：2015-08-01 00:00:00

abstract：:A wide array of sensilla are distributed on insect antennae, and they play a variety of important roles. Rice planthoppers, destructive pests on rice, have a unique antenna sensilla structure called the 'sensory plaque organ'. The spineless (ss) gene encodes a bHLH-PAS transcription factor and plays a key role in ante...

journal_title：Insect molecular biology

authors： Li X,Liu FZ,Cai WL,Zhao J,Hua HX,Zou YL

更新日期：2019-04-01 00:00:00

abstract：:Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...

journal_title：Insect molecular biology

authors： Adelman ZN,Anderson MA,Liu M,Zhang L,Myles KM

更新日期：2012-06-01 00:00:00

abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....

journal_title：Insect molecular biology

authors： Pauchet Y,Wilkinson P,Vogel H,Nelson DR,Reynolds SE,Heckel DG,ffrench-Constant RH

更新日期：2010-02-01 00:00:00

abstract：:A gene from the Mediterranean fruit fly (Medfly) Ceratitis capitata, belonging to the hsp70 family of genes, has been cloned and characterized. This gene appears to be an example of a heat shock cognate (hsc) gene based on a variety of structural and functional criteria. The heat shock cognate genes serve critical rol...

journal_title：Insect molecular biology

authors： Thanaphum S,Haymer DS

更新日期：1998-02-01 00:00:00

abstract：:A high number of (GT)n and (CT)n microsatellites were isolated from a partial genomic library of primitively eusocial wasp, Vespula rufa. On average, (GT)n and (CT)n repeats occur in the genome approximately every 8 kb and 2.5 kb respectively. This is the highest density of dinucleotide microsatellites reported in the...

journal_title：Insect molecular biology

authors： Thorén PA,Paxton RJ,Estoup A

更新日期：1995-08-01 00:00:00

abstract：:Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...

journal_title：Insect molecular biology

authors： Pannebakker BA,Niehuis O,Hedley A,Gadau J,Shuker DM

更新日期：2010-02-01 00:00:00

abstract：:To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...

journal_title：Insect molecular biology

authors： Tadano H,Yamazaki Y,Takeuchi H,Kubo T

更新日期：2009-11-01 00:00:00

abstract：:Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...

journal_title：Insect molecular biology

authors： Qu M,Yang Q

更新日期：2012-08-01 00:00:00

abstract：:Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various m...

journal_title：Insect molecular biology

authors： Busch A,Kunert G,Wielsch N,Pauchet Y

更新日期：2018-10-01 00:00:00

abstract：:Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...

journal_title：Insect molecular biology

authors： Hens K,Lemey P,Macours N,Francis C,Huybrechts R

更新日期：2004-12-01 00:00:00

abstract：:Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

journal_title：Insect molecular biology

authors： Zandawala M,Hamoudi Z,Lange AB,Orchard I

更新日期：2015-04-01 00:00:00

abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

journal_title：Insect molecular biology

authors： Biessmann H,Donath J,Walter MF

更新日期：1996-02-01 00:00:00

abstract：:Little is known about when, how or even if the wing development gene network elucidated in Drosophila is deployed in direct-developing insects. Here we identify the wing development genes (as determined in Drosophila) of the pea aphid (Acyrthosiphon pisum), which produces winged or unwinged adults in response to envir...

journal_title：Insect molecular biology

authors： Brisson JA,Ishikawa A,Miura T

更新日期：2010-03-01 00:00:00

abstract：:Target of rapamycin (TOR), a member of the phosphatidylinositol kinase-related kinase family, plays a critical role in the regulation of growth, metabolism, development and survival, at both the cellular and the organismal levels. Two paralogous Tor genes, BmTor1 and BmTor2, were identified as a pair of inverted repea...

journal_title：Insect molecular biology

authors： Zhou S,Zhou Q,Liu Y,Wang S,Wen D,He Q,Wang W,Bendena WG,Li S

更新日期：2010-12-01 00:00:00

abstract：:The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adu...

journal_title：Insect molecular biology

authors： Venancio TM,Cristofoletti PT,Ferreira C,Verjovski-Almeida S,Terra WR

更新日期：2009-02-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:We have used a PCR assay to screen 404 insects and related arthropods for mariner elements using primers corresponding to amino acids conserved between the mariner elements of Drosophila mauritiana and a moth, Hyalophora cecropia. Potential mariner elements were detected in sixty-three species, representing ten insect...

journal_title：Insect molecular biology

authors： Robertson HM,MacLeod EG

更新日期：1993-01-01 00:00:00

abstract：:ATBP [(A+T)-stretch binding protein] activates the Sarcophaga defense protein genes in an (A+T)-stretch dependent manner as shown previously using a luciferase reporter assay [Aozasa et al. (2001) Eur J Biochem, 268, 2506-2511]. We identified the Drosophila homologue of ATBP. Drosophila ATBP has 388 amino acid residue...

journal_title：Insect molecular biology

authors： Aozasa N,Kubo T,Sekimizu K,Natori S

更新日期：2002-02-01 00:00:00

abstract：:The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of ...

journal_title：Insect molecular biology

authors： Gill M,Ellar D

更新日期：2002-12-01 00:00:00

abstract：:In the ant Camponotus festinatus, two different hexamerins accumulate stage-specifically during the late larval period and at various times in adults. These hexamerins serve as storage proteins and play important roles in brood nourishment and colony founding. We report an analysis of the cDNA sequence of C. festinatu...

journal_title：Insect molecular biology

authors： Martínez T,Burmester T,Veenstra JA,Wheeler D

更新日期：2000-08-01 00:00:00

abstract：:The products of the maternal-effect genes, nanos (nos) and oskar (osk), are important for the development of germ cells in insects. Furthermore, these genes have been proposed as candidates for donating functional DNA regulatory sequences for use in gene drive systems to control transmission of mosquito-borne pathogen...

journal_title：Insect molecular biology

authors： Juhn J,Marinotti O,Calvo E,James AA

更新日期：2008-09-01 00:00:00