The D7 family of salivary proteins in blood sucking diptera.

abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...

journal_title：Insect molecular biology

authors： Vyazunova I,Wessley V,Kim M,Lan Q

更新日期：2007-06-01 00:00:00

abstract：:In the present study, we characterized a full-length cDNA encoding a putative iron-binding protein transferrin from the lepidopteran Mediterranean flour moth (EkTrf, 2397 bp). The putative EkTrf is 683 amino acids with a molecular mass of approximately 76 kDa. The deduced amino acid sequence showed significant homolog...

journal_title：Insect molecular biology

authors： Guz N,Kilincer N,Aksoy S

更新日期：2012-04-01 00:00:00

abstract：:RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

journal_title：Insect molecular biology

authors： Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

更新日期：2017-06-01 00:00:00

abstract：:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

journal_title：Insect molecular biology

authors： Wang J,Ren X,Miller TA,Park Y

更新日期：2006-08-01 00:00:00

abstract：:The hormone 20-hydroxyecdysone is fundamental for regulating moulting and metamorphosis in immature insects, and it plays a role in physiological regulation in adult insects. This hormone acts by binding and activating a receptor, the ecdysone receptor, which is part of the nuclear receptor gene superfamily. Here, we ...

journal_title：Insect molecular biology

authors： Nascimento PVP,Almeida-Oliveira F,Macedo-Silva A,Ausina P,Motinha C,Sola-Penna M,Majerowicz D

更新日期：2021-01-17 00:00:00

abstract：:Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monope...

journal_title：Insect molecular biology

authors： Saikia M,Singh YT,Bhattacharya A,Mazumdar-Leighton S

更新日期：2011-02-01 00:00:00

abstract：:Apis mellifera plays crucial roles in maintaining the balance of global ecosystems and stability of agricultural systems by helping pollination of flowering plants, including many crops. In recent years, this balance has been disrupted greatly by some pesticides, which results in great losses of honeybees worldwide. P...

journal_title：Insect molecular biology

authors： Hu YT,Tang CK,Wu CP,Wu PC,Yang EC,Tai CC,Wu YL

更新日期：2018-08-01 00:00:00

abstract：:Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...

journal_title：Insect molecular biology

authors： Russell CL,Heesom KJ,Arthur CJ,Helps CR,Mellor PS,Day MJ,Torsteinsdottir S,Björnsdóttir TS,Wilson AD

更新日期：2009-06-01 00:00:00

abstract：:RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major ...

journal_title：Insect molecular biology

authors： Balakrishna Pillai A,Nagarajan U,Mitra A,Krishnan U,Rajendran S,Hoti SL,Mishra RK

更新日期：2017-04-01 00:00:00

abstract：:HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...

journal_title：Insect molecular biology

authors： Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal C

更新日期：2000-04-01 00:00:00

abstract：:A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila me...

journal_title：Insect molecular biology

authors： Reiss RA,James AA

更新日期：1993-01-01 00:00:00

abstract：:Myosuppressins are a family of the FMRFamide-like peptides. They have been characterized in many insects and shown to inhibit visceral muscle contraction. Rhodnius prolixus possesses an unusual myosuppressin in that the typical FLRFamide C-terminal motif is unique and ends with FMRFamide. In the present study, we isol...

journal_title：Insect molecular biology

authors： Lee D,James TE,Lange AB

更新日期：2015-02-01 00:00:00

abstract：:Age-dependent division of labour in honeybees was shown to be connected to sensory response thresholds. Foragers show a higher gustatory responsiveness than nurse bees. It is generally assumed that nutrition-related signalling pathways underlie this behavioural plasticity. Here, one important candidate gene is the for...

journal_title：Insect molecular biology

authors： Thamm M,Sturm K,Schlossmann J,Scheiner R

更新日期：2018-12-01 00:00:00

abstract：:Adaptive polymorphism may be common in immune system genes as co-evolutionary interactions foster diversity; either through ongoing positive selection (arms races), or balancing selection. DNA sequence diversity in two putative immune system genes was examined in species of the genus Anopheles and from Aedes aegypti. ...

journal_title：Insect molecular biology

authors： Little TJ,Cobbe N

更新日期：2005-12-01 00:00:00

abstract：:Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...

journal_title：Insect molecular biology

authors： Traver BE,Anderson MA,Adelman ZN

更新日期：2009-10-01 00:00:00

abstract：:The biogenic amine receptor genes constitute an ancient and highly divergent family within the larger superfamily of G-protein-coupled receptors. These receptors play a central role in modulating nerve cell activity and thus behaviour. Because the honey bee offers numerous advantages for behavioural studies we endeavo...

journal_title：Insect molecular biology

authors： Ebert PR,Rowland JE,Toma DP

更新日期：1998-05-01 00:00:00

abstract：:The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...

journal_title：Insect molecular biology

authors： Lan Q,Wu Z,Riddiford LM

更新日期：1997-02-01 00:00:00

abstract：:The maternally inherited bacterium Wolbachia is well known for spreading in natural populations by manipulating the reproduction of its arthropod hosts, but can also have mutualist effects that increase host fitness. In mosquitoes and Drosophila some Wolbachia strains can lead to an increase in survival of virus-infec...

journal_title：Insect molecular biology

authors： Cattel J,Martinez J,Jiggins F,Mouton L,Gibert P

更新日期：2016-10-01 00:00:00

abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

journal_title：Insect molecular biology

authors： Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

更新日期：1994-08-01 00:00:00

abstract：:Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...

journal_title：Insect molecular biology

authors： Yang F,Zhu B,Liu J,Liu Y,Jiang C,Sheng Q,Qiu J,Nie Z

更新日期：2020-02-01 00:00:00

abstract：:Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...

journal_title：Insect molecular biology

authors： Tsubota T,Minakuchi C,Nakakura T,Shinoda T,Shiotsuki T

更新日期：2010-08-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...

journal_title：Insect molecular biology

authors： Nagoshi RN,Meagher R

更新日期：2003-10-01 00:00:00

abstract：:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...

journal_title：Insect molecular biology

authors： Pedra JH,Brandt A,Westerman R,Lobo N,Li HM,Romero-Severson J,Murdock LL,Pittendrigh BR

更新日期：2003-08-01 00:00:00

abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....

journal_title：Insect molecular biology

authors： Pauchet Y,Wilkinson P,Vogel H,Nelson DR,Reynolds SE,Heckel DG,ffrench-Constant RH

更新日期：2010-02-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prop...

journal_title：Insect molecular biology

authors： Paskewitz SM,Reese-Stardy S,Gorman MJ

更新日期：1999-08-01 00:00:00

abstract：:PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...

journal_title：Insect molecular biology

authors： Thompson GJ,Crozier YC,Crozier RH

更新日期：2003-02-01 00:00:00

abstract：:The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...

journal_title：Insect molecular biology

authors： Reim T,Scheiner R

更新日期：2014-12-01 00:00:00

abstract：:The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-ty...

journal_title：Insect molecular biology

authors： Bai X,Zeng T,Ni XY,Su HA,Huang J,Ye GY,Lu YY,Qi YX

更新日期：2019-12-01 00:00:00

abstract：:Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDN...

journal_title：Insect molecular biology

authors： Gaines PJ,Sampson CM,Rushlow KE,Stiegler GL

更新日期：1999-02-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00