Abstract：
:The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prophenol oxidase activating enzyme (pPAE) from Manduca sexta. The catalytic domain is at the carboxy terminus and has the conserved serine, histidine and aspartic acid residues found in serine proteases as well as six cysteines common to invertebrate enzymes. The amino terminus contains critical cysteines that define a clip (=disulphide knot) domain which places this gene product in a subfamily of regulatory serine proteases that includes not only easter and pPAE but also the Drosophila proteins masquerade, stubble and snake as well as proclotting enzyme and factor B from the horseshoe crab. In situ hybridization to the polytene chromosomes detects a single band at 14D and Southern analysis with a probe from the 5' end of the gene confirms the single copy status of this gene. Northern analysis reveals changes in transcript abundance during development and following blood feeding. Interestingly, this analysis also shows an increase in transcript levels following wounding or injection of bacteria.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Paskewitz SM,Reese-Stardy S,Gorman MJdoi
10.1046/j.1365-2583.1999.83124.xsubject
Has Abstractpub_date
1999-08-01 00:00:00pages
329-37issue
3eissn
0962-1075issn
1365-2583pii
imb124journal_volume
8pub_type
杂志文章abstract：:Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00915.x
更新日期：2010-02-01 00:00:00
abstract：:The maternally inherited bacterium Wolbachia is well known for spreading in natural populations by manipulating the reproduction of its arthropod hosts, but can also have mutualist effects that increase host fitness. In mosquitoes and Drosophila some Wolbachia strains can lead to an increase in survival of virus-infec...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12245
更新日期：2016-10-01 00:00:00
abstract：:We have used a PCR assay to screen 404 insects and related arthropods for mariner elements using primers corresponding to amino acids conserved between the mariner elements of Drosophila mauritiana and a moth, Hyalophora cecropia. Potential mariner elements were detected in sixty-three species, representing ten insect...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1993.tb00132.x
更新日期：1993-01-01 00:00:00
abstract：:The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2006.00721.x
更新日期：2007-04-01 00:00:00
abstract：:The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily of pheromone/odourant binding proteins. Although D7 proteins are among the most abundant salivary proteins in adult female mosquitoes and sand flies, their role in blood feeding remains elusive. In the present w...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2002.00319.x
更新日期：2002-04-01 00:00:00
abstract：:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00917.x
更新日期：2009-11-01 00:00:00
abstract：:Nuclear ribosomal ITS2 and mitochondrial cytochrome oxidase DNA sequences were analysed from the Byturidae (Coleoptera), which includes the raspberry beetles. The secondary structure of ITS2 was plotted and interspecific changes analysed. Evidence for selection on simple sequence repeats within the ITS2 was found. Phy...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2001.00266.x
更新日期：2001-06-01 00:00:00
abstract：:Some ATP-binding cassette (ABC) transporters of subfamilies B, C and G confer resistance to xenobiotics including insecticides. We identified genes of these subfamilies expressed by the lepidopterans Trichoplusia ni and Bombyx mori. The B. mori genome includes eight, six and 13 ABC-B, -C and -G genes, respectively, wh...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.01064.x
更新日期：2011-04-01 00:00:00
abstract：:The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1997.00151.x
更新日期：1997-02-01 00:00:00
abstract：:RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12301
更新日期：2017-06-01 00:00:00
abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp24D) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 271 amino acid protein that contains the conserved serine, histidine and aspartic acid residues found in serine proteases, and has the highest identity to ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1997.00193.x
更新日期：1997-11-01 00:00:00
abstract：:HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00175.x
更新日期：2000-04-01 00:00:00
abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00157.x
更新日期：1994-05-01 00:00:00
abstract：:Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. T...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00300.x
更新日期：2001-12-01 00:00:00
abstract：:In the ant Camponotus festinatus, two different hexamerins accumulate stage-specifically during the late larval period and at various times in adults. These hexamerins serve as storage proteins and play important roles in brood nourishment and colony founding. We report an analysis of the cDNA sequence of C. festinatu...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00204.x
更新日期：2000-08-01 00:00:00
abstract：:Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, pl...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1993.tb00113.x
更新日期：1993-01-01 00:00:00
abstract：:Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2007.00747.x
更新日期：2007-08-01 00:00:00
abstract：:PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2003.00381.x
更新日期：2003-02-01 00:00:00
abstract：:A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2001.00249.x
更新日期：2001-04-01 00:00:00
abstract：:Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.0962-1075.2004.00520.x
更新日期：2004-12-01 00:00:00
abstract：:Although the Hymenoptera represent a remarkably diverse and socioeconomically important group that is of considerable interest in genome biology, they remain understudied in terms of genome size. This study reports new genome size estimates for 89 species of ants, bees and wasps, representing 17 families and four supe...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.00992.x
更新日期：2010-06-01 00:00:00
abstract：:The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2003.00429.x
更新日期：2003-10-01 00:00:00
abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00165.x
更新日期：1994-08-01 00:00:00
abstract：:Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00561.x
更新日期：2005-06-01 00:00:00
abstract：:The hormone 20-hydroxyecdysone is fundamental for regulating moulting and metamorphosis in immature insects, and it plays a role in physiological regulation in adult insects. This hormone acts by binding and activating a receptor, the ecdysone receptor, which is part of the nuclear receptor gene superfamily. Here, we ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12696
更新日期：2021-01-17 00:00:00
abstract：:Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae, we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding fu...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2002.00350.x
更新日期：2002-10-01 00:00:00
abstract：:Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00284.x
更新日期：2001-10-01 00:00:00
abstract：:Transcriptional activity of nuclear receptors is the result of transactivation capability and the concentration of the receptor protein. The concentration of ecdysteroid receptor (EcR) isoforms, constitutively expressed in mammalian CHO cells, is dependent on a number of factors. As shown previously, ligand binding st...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2012.01144.x
更新日期：2012-06-01 00:00:00
abstract：:Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases (CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12678
更新日期：2021-02-01 00:00:00
abstract：:We recently showed that the Masculinizer gene (Masc) plays a primary role in sex determination in the lepidopteran model insect Bombyx mori. However, it remains unknown whether this Masc protein-dependent sex determination system is conserved amongst lepidopteran insects or within the family Bombycidae. Here we cloned...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12181
更新日期：2015-10-01 00:00:00
