Abstract:
:The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-type strain, using the CRISPR/Cas9 genome editing system, and found that mutants lost pigmentation in the compound eye and their black head spots. We then examined differences in the expression levels of genes associated with melanin pigmentation between mutants and the wild-type strain using quantitative reverse transcription PCR. We found that transcription levels of the Bd-yellow1 were lower in the head of mutants than in the wild-type strain, and there were no significant differences in expression of the other six genes between mutants and the wild type. Since yellow is critical for melanin biosynthesis (Heinze et al., Scientific Reports. 2017;7:4582), the lower levels of expression of Bd-yellow1 in mutants led to reduced dark pigmentation in head spots. Our results provide the first evidence, to our knowledge, that white may play a functional role in cuticle pigmentation by affecting the expression of yellow.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Bai X,Zeng T,Ni XY,Su HA,Huang J,Ye GY,Lu YY,Qi YXdoi
10.1111/imb.12592subject
Has Abstractpub_date
2019-12-01 00:00:00pages
837-849issue
6eissn
0962-1075issn
1365-2583journal_volume
28pub_type
杂志文章abstract::Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.2006.00674.x
更新日期:2006-10-01 00:00:00
abstract::We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other specie...
journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2002.00341.x
更新日期:2002-08-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12538
更新日期:2019-04-01 00:00:00
abstract::In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...
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pub_type: 杂志文章,评审
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journal_title:Insect molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00249.x
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abstract::The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.1365-2583.1994.tb00142.x
更新日期:1993-01-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2003.00400.x
更新日期:2003-04-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12590
更新日期:2019-12-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12244
更新日期:2016-10-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/j.0962-1075.2004.00468.x
更新日期:2004-04-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1046/j.1365-2583.2001.00266.x
更新日期:2001-06-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12585
更新日期:2019-10-01 00:00:00
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journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12117
更新日期:2014-12-01 00:00:00
abstract::Wolbachia is an intracellular bacterium that has aroused intense interest because of its ability to alter the biology of its host in diverse ways. In the two-spotted spider mite, Tetranychus urticae, Wolbachia can induce complex cytoplasmic incompatibility (CI) phenotypes and fitness changes, although little is known ...
journal_title:Insect molecular biology
pub_type: 杂志文章
doi:10.1111/imb.12128
更新日期:2015-02-01 00:00:00
abstract::Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...
journal_title:Insect molecular biology
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
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journal_title:Insect molecular biology
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doi:10.1111/j.1365-2583.2008.00856.x
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abstract::Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...
journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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abstract::Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, pl...
journal_title:Insect molecular biology
pub_type: 杂志文章
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journal_title:Insect molecular biology
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journal_title:Insect molecular biology
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abstract::Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows ...
journal_title:Insect molecular biology
pub_type: 杂志文章
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abstract::We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...
journal_title:Insect molecular biology
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abstract::A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...
journal_title:Insect molecular biology
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abstract::In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...
journal_title:Insect molecular biology
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abstract::The products of the maternal-effect genes, nanos (nos) and oskar (osk), are important for the development of germ cells in insects. Furthermore, these genes have been proposed as candidates for donating functional DNA regulatory sequences for use in gene drive systems to control transmission of mosquito-borne pathogen...
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