CRISPR/Cas9-mediated knockout of the eye pigmentation gene white leads to alterations in colour of head spots in the oriental fruit fly, Bactrocera dorsalis.

Abstract:

:The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-type strain, using the CRISPR/Cas9 genome editing system, and found that mutants lost pigmentation in the compound eye and their black head spots. We then examined differences in the expression levels of genes associated with melanin pigmentation between mutants and the wild-type strain using quantitative reverse transcription PCR. We found that transcription levels of the Bd-yellow1 were lower in the head of mutants than in the wild-type strain, and there were no significant differences in expression of the other six genes between mutants and the wild type. Since yellow is critical for melanin biosynthesis (Heinze et al., Scientific Reports. 2017;7:4582), the lower levels of expression of Bd-yellow1 in mutants led to reduced dark pigmentation in head spots. Our results provide the first evidence, to our knowledge, that white may play a functional role in cuticle pigmentation by affecting the expression of yellow.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Bai X,Zeng T,Ni XY,Su HA,Huang J,Ye GY,Lu YY,Qi YX

doi

10.1111/imb.12592

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

837-849

issue

6

eissn

0962-1075

issn

1365-2583

journal_volume

28

pub_type

杂志文章
  • Proteomic analyses of male contributions to honey bee sperm storage and mating.

    abstract::Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00674.x

    authors: Collins AM,Caperna TJ,Williams V,Garrett WM,Evans JD

    更新日期:2006-10-01 00:00:00

  • Isolation of cDNAs encoding 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase from the mediterranean fruit fly Ceratitis capitata: correlating genetic and physical maps of chromosome 5.

    abstract::We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other specie...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1993.tb00094.x

    authors: Scott MJ,Kriticou D,Robinson AS

    更新日期:1993-01-01 00:00:00

  • Plant allelochemicals differentially regulate Helicoverpa zea cytochrome P450 genes.

    abstract::Four cytochrome P450 genes, CYP6B8, CYP6B9, CYP6B27 and CYP6B28, exist in the Helicoverpa zea genome as two pairs of paralogs that evolved from gene duplication and 5'-polar gene conversion events. RT-PCR gel blot analyses have shown that all of these genes are expressed constitutively in midguts of all larval instars...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2002.00341.x

    authors: Li X,Berenbaum MR,Schuler MA

    更新日期:2002-08-01 00:00:00

  • The function of spineless in antenna and wing development of the brown planthopper, Nilaparvata lugens.

    abstract::A wide array of sensilla are distributed on insect antennae, and they play a variety of important roles. Rice planthoppers, destructive pests on rice, have a unique antenna sensilla structure called the 'sensory plaque organ'. The spineless (ss) gene encodes a bHLH-PAS transcription factor and plays a key role in ante...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12538

    authors: Li X,Liu FZ,Cai WL,Zhao J,Hua HX,Zou YL

    更新日期:2019-04-01 00:00:00

  • Insect genomes: progress and challenges.

    abstract::In the wake of constant improvements in sequencing technologies, numerous insect genomes have been sequenced. Currently, 1219 insect genome-sequencing projects have been registered with the National Center for Biotechnology Information, including 401 that have genome assemblies and 155 with an official gene set of ann...

    journal_title:Insect molecular biology

    pub_type: 杂志文章,评审

    doi:10.1111/imb.12599

    authors: Li F,Zhao X,Li M,He K,Huang C,Zhou Y,Li Z,Walters JR

    更新日期:2019-12-01 00:00:00

  • N- and C-terminal degradation of ecdysteroid receptor isoforms, when transiently expressed in mammalian CHO cells, is regulated by the proteasome and cysteine and threonine proteases.

    abstract::Transcriptional activity of nuclear receptors is the result of transactivation capability and the concentration of the receptor protein. The concentration of ecdysteroid receptor (EcR) isoforms, constitutively expressed in mammalian CHO cells, is dependent on a number of factors. As shown previously, ligand binding st...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2012.01144.x

    authors: Schauer S,Burster T,Spindler-Barth M

    更新日期:2012-06-01 00:00:00

  • The juvenile hormone binding protein of silkworm haemolymph: gene and functional analysis.

    abstract::A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2001.00249.x

    authors: Vermunt AM,Kamimura M,Hirai M,Kiuchi M,Shiotsuki T

    更新日期:2001-04-01 00:00:00

  • Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28S rDNA sequences.

    abstract::The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1994.tb00142.x

    authors: Campbell BC,Steffen-Campbell JD,Werren JH

    更新日期:1993-01-01 00:00:00

  • Dynamics of Rickettsia-tick interactions: identification and characterization of differentially expressed mRNAs in uninfected and infected Dermacentor variabilis.

    abstract::To begin to explore the molecular dynamics of rickettsial tick interaction, subtractive hybridization was used to screen mRNAs in Rickettsia montanensis-infected and uninfected Dermacentor variabilis. We isolated 30 cDNA fragments, 22 of which were up-regulated and eight were down-regulated in response to rickettsial ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00400.x

    authors: Mulenga A,Macaluso KR,Simser JA,Azad AF

    更新日期:2003-04-01 00:00:00

  • Microarray analysis of genes showing variable expression following a blood meal in Anopheles gambiae.

    abstract::A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00567.x

    authors: Marinotti O,Nguyen QK,Calvo E,James AA,Ribeiro JM

    更新日期:2005-08-01 00:00:00

  • Evolution of the neural sex-determination system in insects: does fruitless homologue regulate neural sexual dimorphism in basal insects?

    abstract::In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12590

    authors: Watanabe T

    更新日期:2019-12-01 00:00:00

  • Bombyx mori E26 transformation-specific 2 (BmEts2), an Ets family protein, represses Bombyx mori Rels (BmRels)-mediated promoter activation of antimicrobial peptide genes in the silkworm Bombyx mori.

    abstract::E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12244

    authors: Tanaka H,Sagisaka A,Suzuki N,Yamakawa M

    更新日期:2016-10-01 00:00:00

  • Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina.

    abstract::We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expresse...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.0962-1075.2004.00468.x

    authors: Yamamoto-Kihara M,Kotani E

    更新日期:2004-04-01 00:00:00

  • Phylogeny of raspberry beetles and other Byturidae (Coleoptera).

    abstract::Nuclear ribosomal ITS2 and mitochondrial cytochrome oxidase DNA sequences were analysed from the Byturidae (Coleoptera), which includes the raspberry beetles. The secondary structure of ITS2 was plotted and interspecific changes analysed. Evidence for selection on simple sequence repeats within the ITS2 was found. Phy...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2001.00266.x

    authors: Malloch G,Fenton B,Goodrich MA

    更新日期:2001-06-01 00:00:00

  • Developmental expression and evolution of hexamerin and haemocyanin from Folsomia candida (Collembola).

    abstract::Haemocyanins constitute a group of copper-containing respiratory proteins, and hexamerins were derived from hexapod haemocyanin but lost the ability to transport oxygen and serve as storage proteins. Although hexamerins have been reported in most insect species, none of them has been identified in Collembola, one of t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12585

    authors: Liang Y,Xie W,Luan YX

    更新日期:2019-10-01 00:00:00

  • Homeodomain-interacting protein kinase (Hipk) phosphorylates the small SPOC family protein Spenito.

    abstract::The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12117

    authors: Dewald DN,Steinmetz EL,Walldorf U

    更新日期:2014-12-01 00:00:00

  • How do hosts react to endosymbionts? A new insight into the molecular mechanisms underlying the Wolbachia-host association.

    abstract::Wolbachia is an intracellular bacterium that has aroused intense interest because of its ability to alter the biology of its host in diverse ways. In the two-spotted spider mite, Tetranychus urticae, Wolbachia can induce complex cytoplasmic incompatibility (CI) phenotypes and fitness changes, although little is known ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12128

    authors: Zhang YK,Ding XL,Rong X,Hong XY

    更新日期:2015-02-01 00:00:00

  • Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes.

    abstract::Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2012.01141.x

    authors: Adelman ZN,Anderson MA,Liu M,Zhang L,Myles KM

    更新日期:2012-06-01 00:00:00

  • Septic injury-inducible genes in medicinal maggots of the green blow fly Lucilia sericata.

    abstract::Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00856.x

    authors: Altincicek B,Vilcinskas A

    更新日期:2009-02-01 00:00:00

  • Immunodetection of a brown planthopper (Nilaparvata lugens Stål) salivary catalase-like protein into tissues of rice, Oryza sativa.

    abstract::Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12058

    authors: Petrova A,Smith CM

    更新日期:2014-02-01 00:00:00

  • Cyclorraphan yolk proteins and lepidopteran minor yolk proteins originate from two unrelated lipase families.

    abstract::Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.0962-1075.2004.00520.x

    authors: Hens K,Lemey P,Macours N,Francis C,Huybrechts R

    更新日期:2004-12-01 00:00:00

  • Regulation of the ecdysone receptor, USP, E75 and MHR3 mRNAs by 20-hydroxyecdysone in the GV1 cell line of the tobacco hornworm, Manduca sexta.

    abstract::The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1997.00151.x

    authors: Lan Q,Wu Z,Riddiford LM

    更新日期:1997-02-01 00:00:00

  • Genetic transformation and phylogeny of bacterial symbionts from tsetse.

    abstract::Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, pl...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1993.tb00113.x

    authors: Beard CB,O'Neill SL,Mason P,Mandelco L,Woese CR,Tesh RB,Richards FF,Aksoy S

    更新日期:1993-01-01 00:00:00

  • Co-immunoprecipitation of putative proteins that interact with mosquito proliferating cell nuclear antigen.

    abstract::We have sequenced cDNAs encoding proliferating cell nuclear antigen (PCNA) from Aedes albopictus cells and from Aedes aegypti mosquitoes. The mosquito cDNAs contained an open reading frame encoding a 260 amino acid protein with a calculated mass of 29.0 kDa and a pI of 4.46. There was a single amino acid difference be...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00628.x

    authors: Ma L,Gerenday A,Coley KM,Fallon AM

    更新日期:2006-04-01 00:00:00

  • Inactivated mariner-like elements (MLE) in pink bollworm, Pectinophora gossypiella.

    abstract::We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00585.x

    authors: Wang J,Staten R,Miller TA,Park Y

    更新日期:2005-10-01 00:00:00

  • Genes of the antioxidant system of the honey bee: annotation and phylogeny.

    abstract::Antioxidant enzymes perform a variety of vital functions including the reduction of life-shortening oxidative damage. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. A comparative analysis of honey bee with Drosophila melanogaster and Anopheles gambiae shows ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2006.00695.x

    authors: Corona M,Robinson GE

    更新日期:2006-10-01 00:00:00

  • A new aminopeptidase from diamondback moth provides evidence for a gene duplication event in Lepidoptera.

    abstract::We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1999.820171.x

    authors: Chang WX,Gahan LJ,Tabashnik BE,Heckel DG

    更新日期:1999-05-01 00:00:00

  • The scarlet eye colour gene of the tephritid fruit fly: Bactrocera tryoni and the nature of two eye colour mutations.

    abstract::A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00410.x

    authors: Zhao JT,Bennett CL,Stewart GJ,Frommer M,Raphael KA

    更新日期:2003-06-01 00:00:00

  • A role for alcohol dehydrogenase in the Drosophila immune response?

    abstract::In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00543.x

    authors: Schlenke TA,McKean KA

    更新日期:2005-04-01 00:00:00

  • Gene structure and expression of nanos (nos) and oskar (osk) orthologues of the vector mosquito, Culex quinquefasciatus.

    abstract::The products of the maternal-effect genes, nanos (nos) and oskar (osk), are important for the development of germ cells in insects. Furthermore, these genes have been proposed as candidates for donating functional DNA regulatory sequences for use in gene drive systems to control transmission of mosquito-borne pathogen...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00823.x

    authors: Juhn J,Marinotti O,Calvo E,James AA

    更新日期:2008-09-01 00:00:00