Microsatellite analysis of sperm admixture in honeybee.

abstract：:We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...

journal_title：Insect molecular biology

authors： Chang WX,Gahan LJ,Tabashnik BE,Heckel DG

更新日期：1999-05-01 00:00:00

abstract：:Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

journal_title：Insect molecular biology

authors： Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

更新日期：2019-12-01 00:00:00

abstract：:The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilized for insect germline transformation. The described ...

journal_title：Insect molecular biology

authors： Gregory M,Alphey L,Morrison NI,Shimeld SM

更新日期：2016-06-01 00:00:00

abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

journal_title：Insect molecular biology

authors： Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

更新日期：1994-05-01 00:00:00

abstract：:We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...

journal_title：Insect molecular biology

authors： Wang J,Staten R,Miller TA,Park Y

更新日期：2005-10-01 00:00:00

abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...

journal_title：Insect molecular biology

authors： Vyazunova I,Wessley V,Kim M,Lan Q

更新日期：2007-06-01 00:00:00

abstract：:Baculovirus-host interactions are important models for studying the biological control of lepidopteran pests. Research on baculovirus-host interactions has focussed on baculovirus manipulation of cellular signalling pathways, including the extracellular signal-regulated kinase (ERK) and phosphatidylinositol-3-kinases/...

journal_title：Insect molecular biology

authors： Jin S,Cheng T,Guo Y,Lin P,Zhao P,Liu C,Kusakabe T,Xia Q

更新日期：2018-08-01 00:00:00

abstract：:The expression pattern of the 79B actin gene in Drosophila melanogaster has been inferred previously by means of a reporter gene in which 79B actin promoter sequences drive the lacZ coding sequences. Although the 79B actin gene is expressed primarily in muscles of the thorax and first abdominal segment of the adults o...

journal_title：Insect molecular biology

authors： Ohshima S,Villarimo C,Gailey DA

更新日期：1997-08-01 00:00:00

abstract：:Four cytochrome P450 genes, CYP6B8, CYP6B9, CYP6B27 and CYP6B28, exist in the Helicoverpa zea genome as two pairs of paralogs that evolved from gene duplication and 5'-polar gene conversion events. RT-PCR gel blot analyses have shown that all of these genes are expressed constitutively in midguts of all larval instars...

journal_title：Insect molecular biology

authors： Li X,Berenbaum MR,Schuler MA

更新日期：2002-08-01 00:00:00

abstract：:Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, L...

journal_title：Insect molecular biology

authors： Yang Y,Zhao X,Niu N,Zhao Y,Liu W,Moussian B,Zhang J

更新日期：2020-12-01 00:00:00

abstract：:In Culex pipiens mosquitoes, A2 esterase alleles are co-amplified with B2 esterase alleles in response to selection with organophosphate insecticides. In this study the amplified A2 and B2 sequences were compared between twelve strains from four continents by restriction mapping. The restriction maps were almost ident...

journal_title：Insect molecular biology

authors： Callaghan A,Guillemaud T,Makate N,Raymond M

更新日期：1998-08-01 00:00:00

abstract：:A novel receptor guanylyl cyclase (GC) has been identified from the oriental fruit fly Bactrocera dorsalis (Hendel) and has been designated BdmGC-1. Protein domain analysis revealed that BdmGC-1 possesses a characteristic domain organization similar to all known receptor GCs but with a unique carboxyl-terminal extensi...

journal_title：Insect molecular biology

authors： Chang JC,Yang RB,Chen YH,Lu KH

更新日期：2006-02-01 00:00:00

abstract：:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...

journal_title：Insect molecular biology

authors： Carpes MP,Nunes JF,Sampaio TL,Castro ME,Zanotto PM,Ribeiro BM

更新日期：2009-11-01 00:00:00

abstract：:A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...

journal_title：Insect molecular biology

authors： Vermunt AM,Kamimura M,Hirai M,Kiuchi M,Shiotsuki T

更新日期：2001-04-01 00:00:00

abstract：:Glutathione transferases (GSTs) are a diverse family of enzymes found ubiquitously in aerobic organisms. They play a central role in the detoxification of both endogenous and xenobiotic compounds and are also involved in intracellular transport, biosynthesis of hormones and protection against oxidative stress. Interes...

journal_title：Insect molecular biology

authors： Enayati AA,Ranson H,Hemingway J

更新日期：2005-01-01 00:00:00

abstract：:In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...

journal_title：Insect molecular biology

authors： Watanabe T

更新日期：2019-12-01 00:00:00

abstract：:A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...

journal_title：Insect molecular biology

authors： Zhao JT,Bennett CL,Stewart GJ,Frommer M,Raphael KA

更新日期：2003-06-01 00:00:00

abstract：:Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but ...

journal_title：Insect molecular biology

authors： Provost-Javier KN,Chen S,Rasgon JL

更新日期：2010-08-01 00:00:00

abstract：:Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...

journal_title：Insect molecular biology

authors： Qu M,Yang Q

更新日期：2012-08-01 00:00:00

abstract：:Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...

journal_title：Insect molecular biology

authors： Foy BD,Myles KM,Pierro DJ,Sanchez-Vargas I,Uhlírová M,Jindra M,Beaty BJ,Olson KE

更新日期：2004-02-01 00:00:00

abstract：:The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of ...

journal_title：Insect molecular biology

authors： Gill M,Ellar D

更新日期：2002-12-01 00:00:00

abstract：:A phylogenetic study, based on maximum parsimony, of ten species in the Anopheles punctulatus group of malaria vectors from the south-west Pacific was performed using structural and similarity-based DNA sequence alignments of the nuclear small ribosomal subunit (SSU = 18S). The structural alignment proved to be more i...

journal_title：Insect molecular biology

authors： Beebe NW,Cooper RD,Morrison DA,Ellis JT

更新日期：2000-10-01 00:00:00

abstract：:We aimed to understand the underlying mechanism that regulates successively expressed cuticular protein (CP) genes around pupation in Bombyx mori. Quantitative PCR was conducted to clarify the expression profile of CP genes and ecdysone-responsive transcription factor (ERTF) genes around pupation. Ecdysone pulse treat...

journal_title：Insect molecular biology

authors： Shahin R,Iwanaga M,Kawasaki H

更新日期：2016-04-01 00:00:00

abstract：:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...

journal_title：Insect molecular biology

authors： Shi L,Li B,Paskewitz SM

更新日期：2006-06-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...

journal_title：Insect molecular biology

authors： Norry FM,Sambucetti P,Scannapieco AC,Gomez FH,Loeschcke V

更新日期：2007-08-01 00:00:00

abstract：:Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...

journal_title：Insect molecular biology

authors： Tsubota T,Minakuchi C,Nakakura T,Shinoda T,Shiotsuki T

更新日期：2010-08-01 00:00:00

abstract：:The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM is believed to act as a damage sensor for the ICL-blocked replication fork and also as a molecular platform for FA core complex assembly and interaction with Bloom's syndrome (BS) complex...

journal_title：Insect molecular biology

authors： Sugahara R,Mon H,Lee JM,Kusakabe T

更新日期：2014-04-01 00:00:00

abstract：:Phenotypic diversity is frequently generated by differences in gene expression. In this study, we addressed the relationship between homology in gene expression and phenotype among four species of eusocial wasps. Specifically, we investigated the evolution of caste-specific and sex-specific gene expression patterns as...

journal_title：Insect molecular biology

authors： Hunt BG,Goodisman MA

更新日期：2010-10-01 00:00:00

abstract：:Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, pl...

journal_title：Insect molecular biology

authors： Beard CB,O'Neill SL,Mason P,Mandelco L,Woese CR,Tesh RB,Richards FF,Aksoy S

更新日期：1993-01-01 00:00:00