Cuticular protein and transcription factor genes expressed during prepupal-pupal transition and by ecdysone pulse treatment in wing discs of Bombyx mori.

abstract：:Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

journal_title：Insect molecular biology

authors： Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

更新日期：1992-01-01 00:00:00

abstract：:Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases (CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The...

journal_title：Insect molecular biology

authors： Yang ZM,Yu N,Wang SJ,Korai SK,Liu ZW

更新日期：2021-02-01 00:00:00

abstract：:Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but ...

journal_title：Insect molecular biology

authors： Provost-Javier KN,Chen S,Rasgon JL

更新日期：2010-08-01 00:00:00

abstract：:Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...

journal_title：Insect molecular biology

authors： Sun ZC,Wu M,Miller TA,Han ZJ

更新日期：2008-02-01 00:00:00

abstract：:The whitefly Bemisia tabaci is a major pest to agriculture. Adults are able to fly for long distances and to colonize staple crops, herbs and ornamentals, and to vector viruses belonging to several important taxonomic groups. During their early development, whiteflies mature from eggs through several nymphal stages (i...

journal_title：Insect molecular biology

authors： Bar L,Czosnek H,Sobol I,Ghanim M,Hariton Shalev A

更新日期：2019-10-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Droso...

journal_title：Insect molecular biology

authors： Tarnay JN,Szeri F,Iliás A,Annilo T,Sung C,Le Saux O,Váradi A,Dean M,Boyd CD,Robinow S

更新日期：2004-10-01 00:00:00

abstract：:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

journal_title：Insect molecular biology

authors： Wang J,Ren X,Miller TA,Park Y

更新日期：2006-08-01 00:00:00

abstract：:The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM is believed to act as a damage sensor for the ICL-blocked replication fork and also as a molecular platform for FA core complex assembly and interaction with Bloom's syndrome (BS) complex...

journal_title：Insect molecular biology

authors： Sugahara R,Mon H,Lee JM,Kusakabe T

更新日期：2014-04-01 00:00:00

abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

journal_title：Insect molecular biology

authors： Biessmann H,Donath J,Walter MF

更新日期：1996-02-01 00:00:00

abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...

journal_title：Insect molecular biology

authors： Horard B,Mangé A,Pélissier B,Couble P

更新日期：1994-11-01 00:00:00

abstract：:The fall armyworm, Spodoptera frugiperda, consists of two host strains (rice and corn) that differ in developmental, physiological and behavioural characteristics. However, because the strains are morphologically indistinguishable the investigation of strain-specific behaviour, particularly in the wild, is very diffic...

journal_title：Insect molecular biology

authors： Nagoshi RN,Meagher R

更新日期：2003-10-01 00:00:00

abstract：:We identified a predicted compact cysteine-rich sequence in the honey bee genome that we called 'Raalin'. Raalin transcripts are enriched in the brain of adult honey bee workers and drones, with only minimum expression in other tissues or in pre-adult stages. Open-reading frame (ORF) homologues of Raalin were identifi...

journal_title：Insect molecular biology

authors： Tirosh Y,Morpurgo N,Cohen M,Linial M,Bloch G

更新日期：2012-06-01 00:00:00

abstract：:Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

journal_title：Insect molecular biology

authors： Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

更新日期：2019-12-01 00:00:00

abstract：:Four cytochrome P450 genes, CYP6B8, CYP6B9, CYP6B27 and CYP6B28, exist in the Helicoverpa zea genome as two pairs of paralogs that evolved from gene duplication and 5'-polar gene conversion events. RT-PCR gel blot analyses have shown that all of these genes are expressed constitutively in midguts of all larval instars...

journal_title：Insect molecular biology

authors： Li X,Berenbaum MR,Schuler MA

更新日期：2002-08-01 00:00:00

abstract：:Insect chitinases are hydrolytic enzymes required for the degradation of chitin. They are essential for insect moulting and metamorphosis. In this study, the regulation mechanism of a chitinase gene, Bombyx mori chitinase 5 (BmCHT5), was studied. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that Bm...

journal_title：Insect molecular biology

authors： Zhang X,Zheng S

更新日期：2017-04-01 00:00:00

abstract：:In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...

journal_title：Insect molecular biology

authors： Watanabe T

更新日期：2019-12-01 00:00:00

abstract：:E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...

journal_title：Insect molecular biology

authors： Tanaka H,Sagisaka A,Suzuki N,Yamakawa M

更新日期：2016-10-01 00:00:00

abstract：:We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. T...

journal_title：Insect molecular biology

authors： Pedra JH,Brandt A,Westerman R,Lobo N,Li HM,Romero-Severson J,Murdock LL,Pittendrigh BR

更新日期：2003-08-01 00:00:00

abstract：:The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...

journal_title：Insect molecular biology

authors： Dewald DN,Steinmetz EL,Walldorf U

更新日期：2014-12-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prop...

journal_title：Insect molecular biology

authors： Paskewitz SM,Reese-Stardy S,Gorman MJ

更新日期：1999-08-01 00:00:00

abstract：:The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...

journal_title：Insect molecular biology

authors： Reim T,Scheiner R

更新日期：2014-12-01 00:00:00

abstract：:Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...

journal_title：Insect molecular biology

authors： Altincicek B,Vilcinskas A

更新日期：2009-02-01 00:00:00

abstract：:We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other specie...

journal_title：Insect molecular biology

authors： Scott MJ,Kriticou D,Robinson AS

更新日期：1993-01-01 00:00:00

abstract：:A transferrin (Tf) gene has been predicted from an expressed sequence tag of the diamondback moth, Plutella xylostella. It encodes 681 amino acid residues that share 80-90% sequence homologies with other lepidopteran Tfs. The gene was constitutively expressed in all developmental stages of P. xylostella. Double-strand...

journal_title：Insect molecular biology

authors： Kim J,Kim Y

更新日期：2010-08-01 00:00:00

abstract：:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...

journal_title：Insect molecular biology

authors： Carpes MP,Nunes JF,Sampaio TL,Castro ME,Zanotto PM,Ribeiro BM

更新日期：2009-11-01 00:00:00

abstract：:HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...

journal_title：Insect molecular biology

authors： Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal C

更新日期：2000-04-01 00:00:00

abstract：:RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

journal_title：Insect molecular biology

authors： Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

更新日期：2017-06-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:Baculovirus-host interactions are important models for studying the biological control of lepidopteran pests. Research on baculovirus-host interactions has focussed on baculovirus manipulation of cellular signalling pathways, including the extracellular signal-regulated kinase (ERK) and phosphatidylinositol-3-kinases/...

journal_title：Insect molecular biology

authors： Jin S,Cheng T,Guo Y,Lin P,Zhao P,Liu C,Kusakabe T,Xia Q

更新日期：2018-08-01 00:00:00

abstract：:The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST of Bombyx mori (bmGSTO) was shown to be induced after exposure to various environmental stresses. A soluble form of recombinant protein (rbmGSTO) was functionally ...

journal_title：Insect molecular biology

authors： Yamamoto K,Teshiba S,Shigeoka Y,Aso Y,Banno Y,Fujiki T,Katakura Y

更新日期：2011-06-01 00:00:00