Evolution of the neural sex-determination system in insects: does fruitless homologue regulate neural sexual dimorphism in basal insects?

abstract：:ATBP [(A+T)-stretch binding protein] activates the Sarcophaga defense protein genes in an (A+T)-stretch dependent manner as shown previously using a luciferase reporter assay [Aozasa et al. (2001) Eur J Biochem, 268, 2506-2511]. We identified the Drosophila homologue of ATBP. Drosophila ATBP has 388 amino acid residue...

journal_title：Insect molecular biology

authors： Aozasa N,Kubo T,Sekimizu K,Natori S

更新日期：2002-02-01 00:00:00

abstract：:RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

journal_title：Insect molecular biology

authors： Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

更新日期：2017-06-01 00:00:00

abstract：:Target of rapamycin (TOR), a member of the phosphatidylinositol kinase-related kinase family, plays a critical role in the regulation of growth, metabolism, development and survival, at both the cellular and the organismal levels. Two paralogous Tor genes, BmTor1 and BmTor2, were identified as a pair of inverted repea...

journal_title：Insect molecular biology

authors： Zhou S,Zhou Q,Liu Y,Wang S,Wen D,He Q,Wang W,Bendena WG,Li S

更新日期：2010-12-01 00:00:00

abstract：:Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...

journal_title：Insect molecular biology

authors： Quan GX,Kanda T,Tamura T

更新日期：2002-06-01 00:00:00

abstract：:We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other specie...

journal_title：Insect molecular biology

authors： Scott MJ,Kriticou D,Robinson AS

更新日期：1993-01-01 00:00:00

abstract：:The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...

journal_title：Insect molecular biology

authors： Zhang F,Li Q,Chen X,Huo Y,Guo H,Song Z,Cui F,Zhang L,Fang R

更新日期：2016-08-01 00:00:00

abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....

journal_title：Insect molecular biology

authors： Pauchet Y,Wilkinson P,Vogel H,Nelson DR,Reynolds SE,Heckel DG,ffrench-Constant RH

更新日期：2010-02-01 00:00:00

abstract：:Sperm usage was investigated in an instrumentally inseminated honeybee queen. Her progeny were examined in the first 3 months of the egg-laying period using a microsatellite marker. Frequencies of different subfamilies differed significantly from one month to another. However, there was no evidence for sperm displacem...

journal_title：Insect molecular biology

authors： Franck P,Coussy H,Le Conte Y,Solignac M,Garnery L,Cornuet JM

更新日期：1999-08-01 00:00:00

abstract：:Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...

journal_title：Insect molecular biology

authors： Norry FM,Sambucetti P,Scannapieco AC,Gomez FH,Loeschcke V

更新日期：2007-08-01 00:00:00

abstract：:Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monope...

journal_title：Insect molecular biology

authors： Saikia M,Singh YT,Bhattacharya A,Mazumdar-Leighton S

更新日期：2011-02-01 00:00:00

abstract：:The products of the maternal-effect genes, nanos (nos) and oskar (osk), are important for the development of germ cells in insects. Furthermore, these genes have been proposed as candidates for donating functional DNA regulatory sequences for use in gene drive systems to control transmission of mosquito-borne pathogen...

journal_title：Insect molecular biology

authors： Juhn J,Marinotti O,Calvo E,James AA

更新日期：2008-09-01 00:00:00

abstract：:Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various m...

journal_title：Insect molecular biology

authors： Busch A,Kunert G,Wielsch N,Pauchet Y

更新日期：2018-10-01 00:00:00

abstract：:Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white(+) cassette (H[w(+), hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one contain...

journal_title：Insect molecular biology

authors： Gomez SP,Handler AM

更新日期：1997-05-01 00:00:00

abstract：:A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

journal_title：Insect molecular biology

authors： Biessmann H,Donath J,Walter MF

更新日期：1996-02-01 00:00:00

abstract：:The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...

journal_title：Insect molecular biology

authors： Dewald DN,Steinmetz EL,Walldorf U

更新日期：2014-12-01 00:00:00

abstract：:Fast and reliable methods for assessing the mobility of the transposable element Minos have been developed. These methods are based on the detection of excision and insertion of Minos transposons from and into plasmids which are co-introduced into cells. Excision is detected by polymerase chain reaction (PCR) with app...

journal_title：Insect molecular biology

authors： Klinakis AG,Loukeris TG,Pavlopoulos A,Savakis C

更新日期：2000-06-01 00:00:00

abstract：:The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-ty...

journal_title：Insect molecular biology

authors： Bai X,Zeng T,Ni XY,Su HA,Huang J,Ye GY,Lu YY,Qi YX

更新日期：2019-12-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp24D) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 271 amino acid protein that contains the conserved serine, histidine and aspartic acid residues found in serine proteases, and has the highest identity to ...

journal_title：Insect molecular biology

authors： Han YS,Salazar CE,Reese-Stardy SR,Cornel A,Gorman MJ,Collins FH,Paskewitz SM

更新日期：1997-11-01 00:00:00

abstract：:Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional t...

journal_title：Insect molecular biology

authors： Green CL,Frommer M

更新日期：2001-08-01 00:00:00

abstract：:Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae, we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding fu...

journal_title：Insect molecular biology

authors： Ranson H,Nikou D,Hutchinson M,Wang X,Roth CW,Hemingway J,Collins FH

更新日期：2002-10-01 00:00:00

abstract：:The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM is believed to act as a damage sensor for the ICL-blocked replication fork and also as a molecular platform for FA core complex assembly and interaction with Bloom's syndrome (BS) complex...

journal_title：Insect molecular biology

authors： Sugahara R,Mon H,Lee JM,Kusakabe T

更新日期：2014-04-01 00:00:00

abstract：:Ecdysteroids, as the key growth hormones, regulate moulting, metamorphosis and reproduction in arthropods. Ecdysteroid biosynthesis is catalysed by a series of cytochrome P450 monooxygenases (CYP450s) encoded by Halloween genes, including spook (spo), phantom (phm), disembodied (dib), shadow (sad) and shade (shd). The...

journal_title：Insect molecular biology

authors： Yang ZM,Yu N,Wang SJ,Korai SK,Liu ZW

更新日期：2021-02-01 00:00:00

abstract：:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...

journal_title：Insect molecular biology

authors： Shi L,Li B,Paskewitz SM

更新日期：2006-06-01 00:00:00

abstract：:We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...

journal_title：Insect molecular biology

authors： Wang J,Staten R,Miller TA,Park Y

更新日期：2005-10-01 00:00:00

abstract：:In this paper we describe the peculiar structures and preferential codon usage found in wild silkworm fibroin genes. We determined a 1350 bp nucleotide sequence from the Chinese oak silkworm, Antheraea pernyi. The deduced amino acid sequence was partitioned into thirteen polyalanine-containing repetitive motifs, which...

journal_title：Insect molecular biology

authors： Yukuhiro K,Kanda T,Tamura T

更新日期：1997-02-01 00:00:00

abstract：:Bactrocera tryoni (Queensland fruit fly) are polyphagous horticultural pests of eastern Australia. Heterogametic males contain a sex-determining Y-chromosome thought to be gene poor and repetitive. Here, we report 39 Y-chromosome scaffolds (~700 kb) from B. tryoni identified using genotype-by-sequencing data and whole...

journal_title：Insect molecular biology

authors： Choo A,Nguyen TNM,Ward CM,Chen IY,Sved J,Shearman D,Gilchrist AS,Crisp P,Baxter SW

更新日期：2019-12-01 00:00:00

abstract：:In the present study, we characterized a full-length cDNA encoding a putative iron-binding protein transferrin from the lepidopteran Mediterranean flour moth (EkTrf, 2397 bp). The putative EkTrf is 683 amino acids with a molecular mass of approximately 76 kDa. The deduced amino acid sequence showed significant homolog...

journal_title：Insect molecular biology

authors： Guz N,Kilincer N,Aksoy S

更新日期：2012-04-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following ...

journal_title：Insect molecular biology

authors： Foy BD,Myles KM,Pierro DJ,Sanchez-Vargas I,Uhlírová M,Jindra M,Beaty BJ,Olson KE

更新日期：2004-02-01 00:00:00

abstract：:Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...

journal_title：Insect molecular biology

authors： Mittapalli O,Stuart JJ,Shukle RH

更新日期：2005-06-01 00:00:00