Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.

Abstract:

:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of homology with a functionally characterized peptide from Musca domestica (Daquinag et al., Proc Natl Acad Sci USA 1995; 92: 2964-2968). The 335 amino acid protein predicted from the A. gambiae cDNA consists of five tandemly arranged inhibitor motifs. The A. gambiae POI gene was expressed in all mosquito stages from egg to adult. POI transcript levels were high in the fat body and were measurable but comparatively reduced in the midgut. The POI transcript level increased after wounding or Sephadex bead injection. Gene knockdown did not result in faster or more extensive bead melanization but did result in more extensive melanization of wound sites following a thoracic bead injection.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Shi L,Li B,Paskewitz SM

doi

10.1111/j.1365-2583.2006.00643.x

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

313-20

issue

3

eissn

0962-1075

issn

1365-2583

pii

IMB643

journal_volume

15

pub_type

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