Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.

abstract：:PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino aci...

journal_title：Insect molecular biology

authors： Thompson GJ,Crozier YC,Crozier RH

更新日期：2003-02-01 00:00:00

abstract：:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The seq...

journal_title：Insect molecular biology

authors： Carpes MP,Nunes JF,Sampaio TL,Castro ME,Zanotto PM,Ribeiro BM

更新日期：2009-11-01 00:00:00

abstract：:Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae, we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding fu...

journal_title：Insect molecular biology

authors： Ranson H,Nikou D,Hutchinson M,Wang X,Roth CW,Hemingway J,Collins FH

更新日期：2002-10-01 00:00:00

abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...

journal_title：Insect molecular biology

authors： Horard B,Mangé A,Pélissier B,Couble P

更新日期：1994-11-01 00:00:00

abstract：:Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...

journal_title：Insect molecular biology

authors： Traver BE,Anderson MA,Adelman ZN

更新日期：2009-10-01 00:00:00

abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...

journal_title：Insect molecular biology

authors： Marshall SD,Gatehouse LN,Becher SA,Christeller JT,Gatehouse HS,Hurst MR,Boucias DG,Jackson TA

更新日期：2008-06-01 00:00:00

abstract：:HindIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica, reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. HindIII rDNA fragment polym...

journal_title：Insect molecular biology

authors： Mukha DV,Sidorenko AP,Lazebnaya IV,Wiegmann BM,Schal C

更新日期：2000-04-01 00:00:00

abstract：:ATBP [(A+T)-stretch binding protein] activates the Sarcophaga defense protein genes in an (A+T)-stretch dependent manner as shown previously using a luciferase reporter assay [Aozasa et al. (2001) Eur J Biochem, 268, 2506-2511]. We identified the Drosophila homologue of ATBP. Drosophila ATBP has 388 amino acid residue...

journal_title：Insect molecular biology

authors： Aozasa N,Kubo T,Sekimizu K,Natori S

更新日期：2002-02-01 00:00:00

abstract：:We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...

journal_title：Insect molecular biology

authors： Sinclair BJ,Gibbs AG,Roberts SP

更新日期：2007-08-01 00:00:00

abstract：:Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, L...

journal_title：Insect molecular biology

authors： Yang Y,Zhao X,Niu N,Zhao Y,Liu W,Moussian B,Zhang J

更新日期：2020-12-01 00:00:00

abstract：:Two genes encoding sterol carrier protein-2 like proteins are identified from fourth instar cDNAs of the yellow fever mosquito, Aedes aegypti. The predicted AeSCP-2like1 (AeSCP-2L1) and AeSCP-2like2 (AeSCP-2L2) proteins are small, acidic and lacking the peroxisomal targeting sequence at the C-termini. Purified recombi...

journal_title：Insect molecular biology

authors： Vyazunova I,Wessley V,Kim M,Lan Q

更新日期：2007-06-01 00:00:00

abstract：:RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major ...

journal_title：Insect molecular biology

authors： Balakrishna Pillai A,Nagarajan U,Mitra A,Krishnan U,Rajendran S,Hoti SL,Mishra RK

更新日期：2017-04-01 00:00:00

abstract：:Horizontal transfer (HT), the exchange of genetic material between species, plays important roles in transposon biology and genome evolution. In this study, we provide the first documented example of a new Academ transposon involved in recent and distant HTs into the genomes of species belonging to seven different ord...

journal_title：Insect molecular biology

authors： Zhang HH,Shen YH,Xiong XM,Han MJ,Qi DW,Zhang XG

更新日期：2016-06-01 00:00:00

abstract：:Odorant-binding proteins (OBPs) are important molecular players in insect olfaction, which has a great influence on the host-seeking behaviour of mosquitoes and other disease vectors. The mRNA level of the Anopheles gambiae Obp7 gene (Agam-Obp7) is higher in the adult female antennae and is slightly reduced in the fem...

journal_title：Insect molecular biology

authors： Sengul MS,Tu Z

更新日期：2008-12-01 00:00:00

abstract：:Adaptive polymorphism may be common in immune system genes as co-evolutionary interactions foster diversity; either through ongoing positive selection (arms races), or balancing selection. DNA sequence diversity in two putative immune system genes was examined in species of the genus Anopheles and from Aedes aegypti. ...

journal_title：Insect molecular biology

authors： Little TJ,Cobbe N

更新日期：2005-12-01 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of a serine protease (AgSp14D1) from the human malaria vector, Anopheles gambiae, is presented. The gene product is a 360 amino acid protein that contains two domains and has the highest sequence similarity to the Drosophila melanogaster serine protease easter and to prop...

journal_title：Insect molecular biology

authors： Paskewitz SM,Reese-Stardy S,Gorman MJ

更新日期：1999-08-01 00:00:00

abstract：:Ligand-gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate-gated chloride channel (MdGluCl-alpha) and a gamma-aminobutyric acid (GABA)-gated chloride channel (MdRdl), were cloned from house-flies (Musca domestica L.). These genes w...

journal_title：Insect molecular biology

authors： Eguchi Y,Ihara M,Ochi E,Shibata Y,Matsuda K,Fushiki S,Sugama H,Hamasaki Y,Niwa H,Wada M,Ozoe F,Ozoe Y

更新日期：2006-12-01 00:00:00

abstract：:cDNAs of Anopheles gambiae Defensin 2 (AgDef2), Defensin 3 (AgDef3) and Defensin 4 (AgDef4), identified in the genome sequence, have been characterized and their expression profiles investigated. In contrast to both typical defensins and insect antimicrobial peptides generally, the newly identified defensins were not ...

journal_title：Insect molecular biology

authors： Meredith JM,Hurd H,Lehane MJ,Eggleston P

更新日期：2008-04-01 00:00:00

abstract：:The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...

journal_title：Insect molecular biology

authors： Reim T,Scheiner R

更新日期：2014-12-01 00:00:00

abstract：:A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis ...

journal_title：Insect molecular biology

authors： Sayed A,Nekl ER,Siqueira HA,Wang HC,Ffrench-Constant RH,Bagley M,Siegfried BD

更新日期：2007-10-01 00:00:00

abstract：:Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...

journal_title：Insect molecular biology

authors： Yang F,Zhu B,Liu J,Liu Y,Jiang C,Sheng Q,Qiu J,Nie Z

更新日期：2020-02-01 00:00:00

abstract：:A phylogenetic study, based on maximum parsimony, of ten species in the Anopheles punctulatus group of malaria vectors from the south-west Pacific was performed using structural and similarity-based DNA sequence alignments of the nuclear small ribosomal subunit (SSU = 18S). The structural alignment proved to be more i...

journal_title：Insect molecular biology

authors： Beebe NW,Cooper RD,Morrison DA,Ellis JT

更新日期：2000-10-01 00:00:00

abstract：:We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

journal_title：Insect molecular biology

authors： Kiya T,Kunieda T,Kubo T

更新日期：2008-09-01 00:00:00

abstract：:Many hemimetabolous insects produce their own cellulase enzymes from the glycoside hydrolase family 9, first observed in termites and cockroaches. Phasmatodea have multiple cellulases, some of which are multifunctional and can degrade xylan or xyloglucan. To discover when these abilities evolved, we identified cellula...

journal_title：Insect molecular biology

authors： Shelomi M,Wipfler B,Zhou X,Pauchet Y

更新日期：2020-02-01 00:00:00

abstract：:Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...

journal_title：Insect molecular biology

authors： Luo C,Shen B,Manley JL,Zheng L

更新日期：2001-10-01 00:00:00

abstract：:Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormon...

journal_title：Insect molecular biology

authors： Lestradet M,Gervasio E,Fraichard S,Dupas S,Alabouvette J,Lemoine A,Charles JP

更新日期：2009-08-01 00:00:00

abstract：:The expression pattern of the 79B actin gene in Drosophila melanogaster has been inferred previously by means of a reporter gene in which 79B actin promoter sequences drive the lacZ coding sequences. Although the 79B actin gene is expressed primarily in muscles of the thorax and first abdominal segment of the adults o...

journal_title：Insect molecular biology

authors： Ohshima S,Villarimo C,Gailey DA

更新日期：1997-08-01 00:00:00

abstract：:Maxadilan is an approximately 7kDa peptide that occurs in the saliva of the sand fly Lutzomyia longipalpis. This peptide is a potent vasodilator and may also have immunomodulatory effects related to the pathogenesis of leishmanial infections. Variation in the primary DNA and inferred amino acid sequence of maxadilan i...

journal_title：Insect molecular biology

authors： Lanzaro GC,Lopes AH,Ribeiro JM,Shoemaker CB,Warburg A,Soares M,Titus RG

更新日期：1999-05-01 00:00:00

abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

journal_title：Insect molecular biology

authors： Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

更新日期：1994-05-01 00:00:00

abstract：:A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila me...

journal_title：Insect molecular biology

authors： Reiss RA,James AA

更新日期：1993-01-01 00:00:00