Cloning and characterization of a putative inhibitor of melanization from Anopheles gambiae.


:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of homology with a functionally characterized peptide from Musca domestica (Daquinag et al., Proc Natl Acad Sci USA 1995; 92: 2964-2968). The 335 amino acid protein predicted from the A. gambiae cDNA consists of five tandemly arranged inhibitor motifs. The A. gambiae POI gene was expressed in all mosquito stages from egg to adult. POI transcript levels were high in the fat body and were measurable but comparatively reduced in the midgut. The POI transcript level increased after wounding or Sephadex bead injection. Gene knockdown did not result in faster or more extensive bead melanization but did result in more extensive melanization of wound sites following a thoracic bead injection.


Insect Mol Biol


Insect molecular biology


Shi L,Li B,Paskewitz SM




Has Abstract


2006-06-01 00:00:00














  • Polymorphisms and fluctuations in copy number of amplified esterase genes in Culex pipiens mosquitoes.

    abstract::In Culex pipiens mosquitoes, A2 esterase alleles are co-amplified with B2 esterase alleles in response to selection with organophosphate insecticides. In this study the amplified A2 and B2 sequences were compared between twelve strains from four continents by restriction mapping. The restriction maps were almost ident...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Callaghan A,Guillemaud T,Makate N,Raymond M

    更新日期:1998-08-01 00:00:00

  • The complete mitochondrial genome sequence of the Mormon cricket (Anabrus simplex: Tettigoniidae: Orthoptera) and an analysis of control region variability.

    abstract::The Anabrus simplex is a swarming plague orthopteran found in western North America. The genome is 15 766 bp in length and genome organization follows the ancestral insect gene arrangement. atp6 lacked any readily identifiable stop codon. Examination of mRNA secondary structure for this gene suggested a stem/loop-medi...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Fenn JD,Cameron SL,Whiting MF

    更新日期:2007-04-01 00:00:00

  • Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti.

    abstract::Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Traver BE,Anderson MA,Adelman ZN

    更新日期:2009-10-01 00:00:00

  • Two-dimensional gel analysis of haemolymph proteins from Plasmodium-melanizing and -non-melanizing strains of Anopheles gambiae.

    abstract::Haemolymph polypeptides from Plasmodium-refractory and -susceptible mosquitoes were compared by one- and two-dimensional gel electrophoresis. The refractory strain of Anopheles gambiae kills malaria parasites by a humoral melanization mechanism whereas the parasites develop normally in susceptible mosquitoes. The two ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Chun J,McMaster J,Han Y,Schwartz A,Paskewitz SM

    更新日期:2000-02-01 00:00:00

  • Two Tor genes in the silkworm Bombyx mori.

    abstract::Target of rapamycin (TOR), a member of the phosphatidylinositol kinase-related kinase family, plays a critical role in the regulation of growth, metabolism, development and survival, at both the cellular and the organismal levels. Two paralogous Tor genes, BmTor1 and BmTor2, were identified as a pair of inverted repea...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Zhou S,Zhou Q,Liu Y,Wang S,Wen D,He Q,Wang W,Bendena WG,Li S

    更新日期:2010-12-01 00:00:00

  • Homeodomain-interacting protein kinase (Hipk) phosphorylates the small SPOC family protein Spenito.

    abstract::The Drosophila homeodomain-interacting protein kinase (Hipk) is a versatile regulator involved in a variety of pathways, such as Notch and Wingless signalling, thereby acting in processes including the promotion of eye development or control of cell numbers in the nervous system. In vertebrates, extensive studies have...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Dewald DN,Steinmetz EL,Walldorf U

    更新日期:2014-12-01 00:00:00

  • Chemosensory proteins used as target for screening behaviourally active compounds in the rice pest Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).

    abstract::Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Duan SG,Li DZ,Wang MQ

    更新日期:2019-02-01 00:00:00

  • Bacterial community structure and diversity in the gut of the muga silkworm, Antheraea assamensis (Lepidoptera: Saturniidae), from India.

    abstract::The muga silkworm, Antheraea assamensis, is exclusively present in the northeastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture-dependent techniques, generic identification using 16S ribosomal RNA probes, diversity analysis and qualitative screening f...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Gandotra S,Kumar A,Naga K,Bhuyan PM,Gogoi DK,Sharma K,Subramanian S

    更新日期:2018-10-01 00:00:00

  • A novel cadherin-like gene from western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), larval midgut tissue.

    abstract::A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Sayed A,Nekl ER,Siqueira HA,Wang HC,Ffrench-Constant RH,Bagley M,Siegfried BD

    更新日期:2007-10-01 00:00:00

  • Preservation of a transgenic strain of the sawfly, Athalia rosae (Hymenoptera) by artificial fertilization using cryopreserved sperm.

    abstract::Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Hatakeyama M,Sumitani M

    更新日期:2005-01-01 00:00:00

  • Plant allelochemicals differentially regulate Helicoverpa zea cytochrome P450 genes.

    abstract::Four cytochrome P450 genes, CYP6B8, CYP6B9, CYP6B27 and CYP6B28, exist in the Helicoverpa zea genome as two pairs of paralogs that evolved from gene duplication and 5'-polar gene conversion events. RT-PCR gel blot analyses have shown that all of these genes are expressed constitutively in midguts of all larval instars...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Li X,Berenbaum MR,Schuler MA

    更新日期:2002-08-01 00:00:00

  • Cellulose degradation in Gastrophysa viridula (Coleoptera: Chrysomelidae): functional characterization of two CAZymes belonging to glycoside hydrolase family 45 reveals a novel enzymatic activity.

    abstract::Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various m...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Busch A,Kunert G,Wielsch N,Pauchet Y

    更新日期:2018-10-01 00:00:00

  • N- and C-terminal degradation of ecdysteroid receptor isoforms, when transiently expressed in mammalian CHO cells, is regulated by the proteasome and cysteine and threonine proteases.

    abstract::Transcriptional activity of nuclear receptors is the result of transactivation capability and the concentration of the receptor protein. The concentration of ecdysteroid receptor (EcR) isoforms, constitutively expressed in mammalian CHO cells, is dependent on a number of factors. As shown previously, ligand binding st...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Schauer S,Burster T,Spindler-Barth M

    更新日期:2012-06-01 00:00:00

  • The function of spineless in antenna and wing development of the brown planthopper, Nilaparvata lugens.

    abstract::A wide array of sensilla are distributed on insect antennae, and they play a variety of important roles. Rice planthoppers, destructive pests on rice, have a unique antenna sensilla structure called the 'sensory plaque organ'. The spineless (ss) gene encodes a bHLH-PAS transcription factor and plays a key role in ante...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Li X,Liu FZ,Cai WL,Zhao J,Hua HX,Zou YL

    更新日期:2019-04-01 00:00:00

  • Molecular analysis of ribosomal DNA from the aphid Amphorophora idaei and an associated fungal organism.

    abstract::The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

    更新日期:1994-08-01 00:00:00

  • X-linked QTL for knockdown resistance to high temperature in Drosophila melanogaster.

    abstract::Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KR...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Norry FM,Sambucetti P,Scannapieco AC,Gomez FH,Loeschcke V

    更新日期:2007-08-01 00:00:00

  • Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in dipteran insects.

    abstract::The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Jordan TV,Shike H,Boulo V,Cedeno V,Fang Q,Davis BS,Jacobs-Lorena M,Higgs S,Fryxell KJ,Burns JC

    更新日期:1998-08-01 00:00:00

  • Molecular characterization of a prophenoloxidase cDNA from the malaria mosquito Anopheles stephensi.

    abstract::Some refractory anopheline mosquitoes are capable of killing Plasmodium, the causative agent of malaria, by melanotic encapsulation of invading ookinetes. Phenoloxidase (PO) appears to be involved in the formation of melanin and toxic metabolites in the surrounding capsule. A cDNA encoding Anopheles stephensi propheno...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Cui L,Luckhart S,Rosenberg R

    更新日期:2000-04-01 00:00:00

  • Cowpea bruchid midgut transcriptome response to a soybean cystatin--costs and benefits of counter-defence.

    abstract::The insect digestive system is the first line of defence protecting cells and tissues of the body from a broad spectrum of toxins and antinutritional factors in its food. To gain insight into the nature and breadth of genes involved in adaptation to dietary challenge, a collection of 20 352 cDNAs was prepared from the...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Chi YH,Salzman RA,Balfe S,Ahn JE,Sun W,Moon J,Yun DJ,Lee SY,Higgins TJ,Pittendrigh B,Murdock LL,Zhu-Salzman K

    更新日期:2009-02-01 00:00:00

  • A viral histone H4 suppresses expression of a transferrin that plays a role in the immune response of the diamondback moth, Plutella xylostella.

    abstract::A transferrin (Tf) gene has been predicted from an expressed sequence tag of the diamondback moth, Plutella xylostella. It encodes 681 amino acid residues that share 80-90% sequence homologies with other lepidopteran Tfs. The gene was constitutively expressed in all developmental stages of P. xylostella. Double-strand...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Kim J,Kim Y

    更新日期:2010-08-01 00:00:00

  • Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L.

    abstract::To elucidate the molecular mechanisms underlying honeybee social behaviours, we identified a novel gene, Nb-1, whose expression in the worker brain changes according to the age-dependent division of labour in normal colonies. The open reading frames contained in the Nb-1 cDNA were not conserved in the homologue of a r...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Tadano H,Yamazaki Y,Takeuchi H,Kubo T

    更新日期:2009-11-01 00:00:00

  • Phylogeny of raspberry beetles and other Byturidae (Coleoptera).

    abstract::Nuclear ribosomal ITS2 and mitochondrial cytochrome oxidase DNA sequences were analysed from the Byturidae (Coleoptera), which includes the raspberry beetles. The secondary structure of ITS2 was plotted and interspecific changes analysed. Evidence for selection on simple sequence repeats within the ITS2 was found. Phy...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Malloch G,Fenton B,Goodrich MA

    更新日期:2001-06-01 00:00:00

  • Molecular characterization of N-methyl-d-aspartate receptor from Bemisia tabaci.

    abstract::The N-methyl-d-aspartate receptors (NMDARs) are ionotropic ligand gated channels that are highly permeable to calcium ions. In insects, NMDARs are associated with glutamatergic neurotransmission governing diverse physiological and biological processes like vitellogenesis and ovarian development. Therefore, NMDAR may a...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Dixit S,Thakur N,Shukla A,Upadhyay SK,C Verma P

    更新日期:2020-12-27 00:00:00

  • CRISPR/Cas9-mediated knockout of the eye pigmentation gene white leads to alterations in colour of head spots in the oriental fruit fly, Bactrocera dorsalis.

    abstract::The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-ty...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Bai X,Zeng T,Ni XY,Su HA,Huang J,Ye GY,Lu YY,Qi YX

    更新日期:2019-12-01 00:00:00

  • Multifunctional cellulase enzymes are ancestral in Polyneoptera.

    abstract::Many hemimetabolous insects produce their own cellulase enzymes from the glycoside hydrolase family 9, first observed in termites and cockroaches. Phasmatodea have multiple cellulases, some of which are multifunctional and can degrade xylan or xyloglucan. To discover when these abilities evolved, we identified cellula...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Shelomi M,Wipfler B,Zhou X,Pauchet Y

    更新日期:2020-02-01 00:00:00

  • Identification and isolation of cDNA clones encoding the abundant secreted proteins in the saliva proteome of Culicoides nubeculosus.

    abstract::Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Russell CL,Heesom KJ,Arthur CJ,Helps CR,Mellor PS,Day MJ,Torsteinsdottir S,Björnsdóttir TS,Wilson AD

    更新日期:2009-06-01 00:00:00

  • A new aminopeptidase from diamondback moth provides evidence for a gene duplication event in Lepidoptera.

    abstract::We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Chang WX,Gahan LJ,Tabashnik BE,Heckel DG

    更新日期:1999-05-01 00:00:00

  • The juvenile hormone binding protein of silkworm haemolymph: gene and functional analysis.

    abstract::A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Vermunt AM,Kamimura M,Hirai M,Kiuchi M,Shiotsuki T

    更新日期:2001-04-01 00:00:00

  • PCR primers for the amplification of four insect mitochondrial gene fragments.

    abstract::Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect m...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Kambhampati S,Smith PT

    更新日期:1995-11-01 00:00:00

  • Gene transcription during exposure to, and recovery from, cold and desiccation stress in Drosophila melanogaster.

    abstract::We exposed adult male Drosophila melanogaster to cold, desiccation or starvation, and examined expression of several genes during exposure and recovery. Frost was expressed during recovery from cold, and was up-regulated during desiccation. Desiccation and starvation (but not cold) elicited increased expression of the...

    journal_title:Insect molecular biology

    pub_type: 杂志文章


    authors: Sinclair BJ,Gibbs AG,Roberts SP

    更新日期:2007-08-01 00:00:00