Isolation of cDNAs encoding 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase from the mediterranean fruit fly Ceratitis capitata: correlating genetic and physical maps of chromosome 5.

Abstract:

:We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other species. The codon usage of the cDNA clones has little bias with the notable exceptions of arginine, glycine and leucine. The chromosomal location of the genes for 6PGD and G6PD were determined by in situ hybridization to salivary gland polytene chromosomes. This localization orients a genetic map of enzymatic loci and illustrates a remarkable similarity in the intra chromosomal order of homologous genes between Drosophila melanogaster and medfly.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Scott MJ,Kriticou D,Robinson AS

doi

10.1111/j.1365-2583.1993.tb00094.x

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

213-22

issue

4

eissn

0962-1075

issn

1365-2583

journal_volume

1

pub_type

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