Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap-3) by a new class-II piggyBac-related insect transposon.

Abstract:

:A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Carpes MP,Nunes JF,Sampaio TL,Castro ME,Zanotto PM,Ribeiro BM

doi

10.1111/j.1365-2583.2009.00917.x

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

747-57

issue

6

eissn

0962-1075

issn

1365-2583

pii

IMB917

journal_volume

18

pub_type

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