Molecular characterization of N-methyl-d-aspartate receptor from Bemisia tabaci.

Abstract:

:The N-methyl-d-aspartate receptors (NMDARs) are ionotropic ligand gated channels that are highly permeable to calcium ions. In insects, NMDARs are associated with glutamatergic neurotransmission governing diverse physiological and biological processes like vitellogenesis and ovarian development. Therefore, NMDAR may act as attractive target for insect pest control. In present study, we performed structural and functional characterization of NMDARs in Bemisia tabaci, a highly invasive crop pest and potent virus vector. We identified that NMDAR consists of three subunits each encoded by single gene in whiteflies which are highly conserved among different insect orders. Expression analysis suggests that subunit 1 (BtNR1) and subunit 2 (BtNR2) are the main functional units. External supplementation of NMDAR ligand or BtNRs silencing was lethal to insects, which suggested that NMDAR function is highly balanced in whiteflies.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Dixit S,Thakur N,Shukla A,Upadhyay SK,C Verma P

doi

10.1111/imb.12690

subject

Has Abstract

pub_date

2020-12-27 00:00:00

eissn

0962-1075

issn

1365-2583

pub_type

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