Septic injury-inducible genes in medicinal maggots of the green blow fly Lucilia sericata.

Abstract:

:Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used the suppression subtractive hybridization method to screen for genes that are differentially expressed in response to septic wounding of sterile second instar larvae of L. sericata. This approach resulted in the identification of 65 novel Lucilia genes including potential signalling proteins (e.g. inhibitor of apoptosis 2 protein) and a number of digestive enzymes including lipases and proteinases. Additionally, we found numerous putative antimicrobial peptides (AMPs), such as a potential Lucilia defensin, diptericin and three novel proline-rich AMPs. The identified genes may facilitate access to both peptides and proteins within the beneficial excretions, secretions and haemolymph of medicinal maggots and provide novel insights into the evolution of innate immunity in Diptera.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Altincicek B,Vilcinskas A

doi

10.1111/j.1365-2583.2008.00856.x

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

119-25

issue

1

eissn

0962-1075

issn

1365-2583

pii

IMB856

journal_volume

18

pub_type

杂志文章
  • An estimate of the number of serine protease genes expressed in sheep blowfly larvae (Lucilia cuprina).

    abstract::A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1994.tb00157.x

    authors: Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

    更新日期:1994-05-01 00:00:00

  • Hidden from the host: Spiroplasma bacteria infecting Drosophila do not cause an immune response, but are suppressed by ectopic immune activation.

    abstract::Insects and other arthropods have an effective innate immune system that can clear infections with bacteria and other microorganisms. Despite this ability, one group of bacteria, the spiroplasmas, survive unharmed within the haemolymph of a wide range of arthropod hosts. We investigated the interaction between one mem...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2003.00380.x

    authors: Hurst GD,Anbutsu H,Kutsukake M,Fukatsu T

    更新日期:2003-02-01 00:00:00

  • Division of labour in honey bees: age- and task-related changes in the expression of octopamine receptor genes.

    abstract::The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12130

    authors: Reim T,Scheiner R

    更新日期:2014-12-01 00:00:00

  • Amblyomma americanum tick saliva serine protease inhibitor 6 is a cross-class inhibitor of serine proteases and papain-like cysteine proteases that delays plasma clotting and inhibits platelet aggregation.

    abstract::We previously demonstrated that Amblyomma americanum tick serine protease inhibitor 6 (AamS6) was secreted into the host during tick feeding and that both its mRNA and protein were ubiquitously and highly expressed during the first 3 days of tick feeding. This study demonstrates that AamS6 is a cross-class inhibitor o...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12024

    authors: Mulenga A,Kim T,Ibelli AM

    更新日期:2013-06-01 00:00:00

  • Vitellogenin gene expression in autogenous Culex tarsalis.

    abstract::Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.00999.x

    authors: Provost-Javier KN,Chen S,Rasgon JL

    更新日期:2010-08-01 00:00:00

  • Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in dipteran insects.

    abstract::The control of insects that transmit disease and damage crops has become increasingly difficult. The ability to genetically engineer insects would facilitate strategies to protect crops and block arthropod vector-borne disease transmission. Transformation vectors based on insect transposable elements have been develop...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1998.00063.x

    authors: Jordan TV,Shike H,Boulo V,Cedeno V,Fang Q,Davis BS,Jacobs-Lorena M,Higgs S,Fryxell KJ,Burns JC

    更新日期:1998-08-01 00:00:00

  • Levels and activity of cyclic guanosine monophosphate-dependent protein kinase in nurse and forager honeybees.

    abstract::Age-dependent division of labour in honeybees was shown to be connected to sensory response thresholds. Foragers show a higher gustatory responsiveness than nurse bees. It is generally assumed that nutrition-related signalling pathways underlie this behavioural plasticity. Here, one important candidate gene is the for...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12520

    authors: Thamm M,Sturm K,Schlossmann J,Scheiner R

    更新日期:2018-12-01 00:00:00

  • Chemosensory proteins used as target for screening behaviourally active compounds in the rice pest Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).

    abstract::Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12532

    authors: Duan SG,Li DZ,Wang MQ

    更新日期:2019-02-01 00:00:00

  • Inducible- and constitutive-type transcript variants of kakusei , a novel non-coding immediate early gene, in the honeybee brain.

    abstract::We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2008.00821.x

    authors: Kiya T,Kunieda T,Kubo T

    更新日期:2008-09-01 00:00:00

  • Genetic mapping of genes conferring permethrin resistance in the malaria vector, Anopheles gambiae.

    abstract::Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associ...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.0962-1075.2004.00495.x

    authors: Ranson H,Paton MG,Jensen B,McCarroll L,Vaughan A,Hogan JR,Hemingway J,Collins FH

    更新日期:2004-08-01 00:00:00

  • Multifunctional cellulase enzymes are ancestral in Polyneoptera.

    abstract::Many hemimetabolous insects produce their own cellulase enzymes from the glycoside hydrolase family 9, first observed in termites and cockroaches. Phasmatodea have multiple cellulases, some of which are multifunctional and can degrade xylan or xyloglucan. To discover when these abilities evolved, we identified cellula...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12614

    authors: Shelomi M,Wipfler B,Zhou X,Pauchet Y

    更新日期:2020-02-01 00:00:00

  • The expression of the HSP90 gene in response to winter and summer diapauses and thermal-stress in the onion maggot, Delia antiqua.

    abstract::The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00602.x

    authors: Chen B,Kayukawa T,Monteiro A,Ishikawa Y

    更新日期:2005-12-01 00:00:00

  • Microarray analysis of genes showing variable expression following a blood meal in Anopheles gambiae.

    abstract::A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) d...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00567.x

    authors: Marinotti O,Nguyen QK,Calvo E,James AA,Ribeiro JM

    更新日期:2005-08-01 00:00:00

  • Mobility assays confirm the broad host-range activity of the Minos transposable element and validate new transformation tools.

    abstract::Fast and reliable methods for assessing the mobility of the transposable element Minos have been developed. These methods are based on the detection of excision and insertion of Minos transposons from and into plasmids which are co-introduced into cells. Excision is detected by polymerase chain reaction (PCR) with app...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.2000.00183.x

    authors: Klinakis AG,Loukeris TG,Pavlopoulos A,Savakis C

    更新日期:2000-06-01 00:00:00

  • Transfection of cultured cells of the cotton boll weevil, Anthonomus grandis, with a heat-shock-promoter-chloramphenicol-acetyltransferase construct.

    abstract::Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1993.tb00108.x

    authors: Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

    更新日期:1992-01-01 00:00:00

  • Regulation of the ecdysone receptor, USP, E75 and MHR3 mRNAs by 20-hydroxyecdysone in the GV1 cell line of the tobacco hornworm, Manduca sexta.

    abstract::The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2583.1997.00151.x

    authors: Lan Q,Wu Z,Riddiford LM

    更新日期:1997-02-01 00:00:00

  • Wolbachia-mediated protection against viruses in the invasive pest Drosophila suzukii.

    abstract::The maternally inherited bacterium Wolbachia is well known for spreading in natural populations by manipulating the reproduction of its arthropod hosts, but can also have mutualist effects that increase host fitness. In mosquitoes and Drosophila some Wolbachia strains can lead to an increase in survival of virus-infec...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12245

    authors: Cattel J,Martinez J,Jiggins F,Mouton L,Gibert P

    更新日期:2016-10-01 00:00:00

  • The genome of the Queensland fruit fly Bactrocera tryoni contains multiple representatives of the mariner family of transposable elements.

    abstract::Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1046/j.0962-1075.2001.00275.x

    authors: Green CL,Frommer M

    更新日期:2001-08-01 00:00:00

  • Molecular characterization of the Anopheles gambiae 2L telomeric region via an integrated transgene.

    abstract::A Drosophila P-element derivative (pUChsneo) integrated into the telomeric region of the left arm of the second chromosome of Anopheles gambiae was used to clone the proximally flanking An. gambiae sequences. Molecular analyses revealed that the pUChsneo construct was partially duplicated and had integrated into a sub...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1996.tb00035.x

    authors: Biessmann H,Donath J,Walter MF

    更新日期:1996-02-01 00:00:00

  • Molecular characterization of a gene encoding juvenile hormone esterase in the red flour beetle, Tribolium castaneum.

    abstract::Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01019.x

    authors: Tsubota T,Minakuchi C,Nakakura T,Shinoda T,Shiotsuki T

    更新日期:2010-08-01 00:00:00

  • Compatible interaction with its rice host leads to enhanced expression of the gamma subunit of oligosaccharyl transferase in the Asian rice gall midge, Orseolia oryzae.

    abstract::The Asian rice gall midge, Orseolia oryzae, is a fast evolving, damaging pest of rice. Understanding the underlying molecular mechanism of interaction between the gall midge and rice will help in devising strategies to control and manage the pest. The present study aims to identify rice-responsive genes in the gall mi...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2011.01085.x

    authors: Sinha DK,Bentur JS,Nair S

    更新日期:2011-10-01 00:00:00

  • Preservation of a transgenic strain of the sawfly, Athalia rosae (Hymenoptera) by artificial fertilization using cryopreserved sperm.

    abstract::Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2004.00526.x

    authors: Hatakeyama M,Sumitani M

    更新日期:2005-01-01 00:00:00

  • Immunodetection of a brown planthopper (Nilaparvata lugens Stål) salivary catalase-like protein into tissues of rice, Oryza sativa.

    abstract::Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant ti...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12058

    authors: Petrova A,Smith CM

    更新日期:2014-02-01 00:00:00

  • Roles of the Laodelphax striatellus Down syndrome cell adhesion molecule in Rice stripe virus infection of its insect vector.

    abstract::The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12226

    authors: Zhang F,Li Q,Chen X,Huo Y,Guo H,Song Z,Cui F,Zhang L,Fang R

    更新日期:2016-08-01 00:00:00

  • Pyrosequencing and characterization of immune response genes from the American dog tick, Dermacentor variabilis (L.).

    abstract::Ticks continue to be a threat to animal and human health, and new and novel control strategies are needed for ticks and tick-borne pathogens. The characterization of the tick-pathogen interface and the tick immune response to microbial infections is fundamental toward the formulation of new control strategies for tick...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2010.01037.x

    authors: Jaworski DC,Zou Z,Bowen CJ,Wasala NB,Madden R,Wang Y,Kocan KM,Jiang H,Dillwith JW

    更新日期:2010-10-01 00:00:00

  • Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor.

    abstract::Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Diptera...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.2005.00561.x

    authors: Mittapalli O,Stuart JJ,Shukle RH

    更新日期:2005-06-01 00:00:00

  • A glutathione S-transferase gene of the vector mosquito, Anopheles gambiae.

    abstract::A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila me...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2583.1993.tb00122.x

    authors: Reiss RA,James AA

    更新日期:1993-01-01 00:00:00

  • Molecular characterization of insulin-like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).

    abstract::Insulin-like peptides (ILPs) including insulin, insulin-like growth factor (IGF) and relaxin are evolutionarily conserved hormones in metazoans, and they are involved in diverse physiological processes. The migratory brown planthopper (BPH), Nilaparvata lugens, encodes four ILP genes (Nlilp1, Nlilp2, Nlilp3 and Nlilp4...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12636

    authors: Xue WH,Liu YL,Jiang YQ,He SF,Wang QQ,Yang ZN,Xu HJ

    更新日期:2020-06-01 00:00:00

  • Developmental expression and evolution of hexamerin and haemocyanin from Folsomia candida (Collembola).

    abstract::Haemocyanins constitute a group of copper-containing respiratory proteins, and hexamerins were derived from hexapod haemocyanin but lost the ability to transport oxygen and serve as storage proteins. Although hexamerins have been reported in most insect species, none of them has been identified in Collembola, one of t...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12585

    authors: Liang Y,Xie W,Luan YX

    更新日期:2019-10-01 00:00:00

  • Molecular basis for odorant receptor tuning: a short C-terminal sequence is necessary and sufficient for selectivity of mosquito Or8.

    abstract::A birth-and-death evolutionary model for odorant receptor gene repertoires presumes the creation of repertoires with the capacity for high-level diversity and rapid ligand specificity change. This changes the recognised odour space, directly affecting fitness-related behaviours and ultimately affecting adaptation to n...

    journal_title:Insect molecular biology

    pub_type: 杂志文章

    doi:10.1111/imb.12176

    authors: Hill SR,Majeed S,Ignell R

    更新日期:2015-08-01 00:00:00