Functional characterization of Musca glutamate- and GABA-gated chloride channels expressed independently and coexpressed in Xenopus oocytes.

abstract：:Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...

journal_title：Insect molecular biology

authors： Altincicek B,Vilcinskas A

更新日期：2009-02-01 00:00:00

abstract：:Insect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N-deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinot...

journal_title：Insect molecular biology

authors： Wu JJ,Chen ZC,Wang YW,Fu KY,Guo WC,Li GQ

更新日期：2019-02-01 00:00:00

abstract：:A cDNA fragment of haemolymph juvenile hormone binding protein (hJHBP) from larvae of Bombyx mori was amplified by RT-PCR using degenerate primers based on the N-terminal amino acid sequence of purified hJHBP and a conserved region near the C-terminus of other lepidopteran hJHBPs. 5'- and 3'-ends were amplified by RAC...

journal_title：Insect molecular biology

authors： Vermunt AM,Kamimura M,Hirai M,Kiuchi M,Shiotsuki T

更新日期：2001-04-01 00:00:00

abstract：:A high number of (GT)n and (CT)n microsatellites were isolated from a partial genomic library of primitively eusocial wasp, Vespula rufa. On average, (GT)n and (CT)n repeats occur in the genome approximately every 8 kb and 2.5 kb respectively. This is the highest density of dinucleotide microsatellites reported in the...

journal_title：Insect molecular biology

authors： Thorén PA,Paxton RJ,Estoup A

更新日期：1995-08-01 00:00:00

abstract：:Aedes aegypti is a major vector of arthropod-borne viruses such as yellow fever virus and dengue viruses. Efforts to discern the function of genes involved in important behaviours, such as vector competence and host seeking through reverse genetics, would greatly benefit from the ability to generate targeted gene disr...

journal_title：Insect molecular biology

authors： Traver BE,Anderson MA,Adelman ZN

更新日期：2009-10-01 00:00:00

abstract：:The insect digestive system is the first line of defence protecting cells and tissues of the body from a broad spectrum of toxins and antinutritional factors in its food. To gain insight into the nature and breadth of genes involved in adaptation to dietary challenge, a collection of 20 352 cDNAs was prepared from the...

journal_title：Insect molecular biology

authors： Chi YH,Salzman RA,Balfe S,Ahn JE,Sun W,Moon J,Yun DJ,Lee SY,Higgins TJ,Pittendrigh B,Murdock LL,Zhu-Salzman K

更新日期：2009-02-01 00:00:00

abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

journal_title：Insect molecular biology

authors： Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

更新日期：1994-08-01 00:00:00

abstract：:Germline transformation using a piggyBac-derived vector is feasible in the sawfly, Athalia rosae. A previously generated transgenic line carrying green fluorescence protein (GFP) genes as reporters was successfully maintained and preserved without consecutive rearing. Sperm taken from males that were frozen directly i...

journal_title：Insect molecular biology

authors： Hatakeyama M,Sumitani M

更新日期：2005-01-01 00:00:00

abstract：:Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicator...

journal_title：Insect molecular biology

authors： Thibault ST,Luu HT,Vann N,Miller TA

更新日期：1999-02-01 00:00:00

abstract：:Expression of heat shock proteins (hsp) in the BRL-AG-3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41 degrees C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacteri...

journal_title：Insect molecular biology

authors： Stiles B,Heilmann J,Sparks RB,Santoso A,Leopold RA

更新日期：1992-01-01 00:00:00

abstract：:Haemocyanins constitute a group of copper-containing respiratory proteins, and hexamerins were derived from hexapod haemocyanin but lost the ability to transport oxygen and serve as storage proteins. Although hexamerins have been reported in most insect species, none of them has been identified in Collembola, one of t...

journal_title：Insect molecular biology

authors： Liang Y,Xie W,Luan YX

更新日期：2019-10-01 00:00:00

abstract：:In the brain of holometabolous insects such as the fruit fly Drosophila melanogaster, the fruitless gene produces sex-specific gene products under the control of the sex-specific splicing cascade and contributes to the formation of the sexually dimorphic circuits. Similar sex-specific gene products of fruitless homolo...

journal_title：Insect molecular biology

authors： Watanabe T

更新日期：2019-12-01 00:00:00

abstract：:Sensory neurone membrane proteins (SNMPs) function as essential cofactors for insect sex pheromone detection. In this study, we report two SNMPs in Ectropis obliqua Prout, a serious geometrid pest that produces typical Type-II sex pheromones. Sequence alignments and phylogenetic analyses showed that EoblSNMP1 and Eobl...

journal_title：Insect molecular biology

authors： Sun L,Wang Q,Zhang Y,Yan Y,Guo H,Xiao Q,Zhang Y

更新日期：2019-06-01 00:00:00

abstract：:Sperm usage was investigated in an instrumentally inseminated honeybee queen. Her progeny were examined in the first 3 months of the egg-laying period using a microsatellite marker. Frequencies of different subfamilies differed significantly from one month to another. However, there was no evidence for sperm displacem...

journal_title：Insect molecular biology

authors： Franck P,Coussy H,Le Conte Y,Solignac M,Garnery L,Cornuet JM

更新日期：1999-08-01 00:00:00

abstract：:E26 transformation-specific (Ets) family transcription factors are known to play roles in various biological phenomena, including immunity, in vertebrates. However, the mechanisms by which Ets proteins contribute to immunity in invertebrates remain poorly understood. In this study, we identified a cDNA encoding BmEts2...

journal_title：Insect molecular biology

authors： Tanaka H,Sagisaka A,Suzuki N,Yamakawa M

更新日期：2016-10-01 00:00:00

abstract：:Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the presumed DDD domain...

journal_title：Insect molecular biology

authors： Sun ZC,Wu M,Miller TA,Han ZJ

更新日期：2008-02-01 00:00:00

abstract：:Cyclobutane pyrimidine dimer (CPD) photolyases repair ultraviolet (UV)-induced DNA damage using blue light. To get insight in the origin of baculovirus CPD photolyase (phr) genes, homologues in the lepidopteran insects Chrysodeixis chalcites, Spodoptera exigua and Trichoplusia ni were identified and characterized. Lep...

journal_title：Insect molecular biology

authors： Biernat MA,Ros VI,Vlak JM,van Oers MM

更新日期：2011-08-01 00:00:00

abstract：:Myosuppressins are a family of the FMRFamide-like peptides. They have been characterized in many insects and shown to inhibit visceral muscle contraction. Rhodnius prolixus possesses an unusual myosuppressin in that the typical FLRFamide C-terminal motif is unique and ends with FMRFamide. In the present study, we isol...

journal_title：Insect molecular biology

authors： Lee D,James TE,Lange AB

更新日期：2015-02-01 00:00:00

abstract：:Insect chitinases are hydrolytic enzymes required for the degradation of chitin. They are essential for insect moulting and metamorphosis. In this study, the regulation mechanism of a chitinase gene, Bombyx mori chitinase 5 (BmCHT5), was studied. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that Bm...

journal_title：Insect molecular biology

authors： Zhang X,Zheng S

更新日期：2017-04-01 00:00:00

abstract：:The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of virus...

journal_title：Insect molecular biology

authors： Zhang F,Li Q,Chen X,Huo Y,Guo H,Song Z,Cui F,Zhang L,Fang R

更新日期：2016-08-01 00:00:00

abstract：:RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

journal_title：Insect molecular biology

authors： Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

更新日期：2017-06-01 00:00:00

abstract：:The N-methyl-d-aspartate receptors (NMDARs) are ionotropic ligand gated channels that are highly permeable to calcium ions. In insects, NMDARs are associated with glutamatergic neurotransmission governing diverse physiological and biological processes like vitellogenesis and ovarian development. Therefore, NMDAR may a...

journal_title：Insect molecular biology

authors： Dixit S,Thakur N,Shukla A,Upadhyay SK,C Verma P

更新日期：2020-12-27 00:00:00

abstract：:Insulin-like peptides (ILPs) including insulin, insulin-like growth factor (IGF) and relaxin are evolutionarily conserved hormones in metazoans, and they are involved in diverse physiological processes. The migratory brown planthopper (BPH), Nilaparvata lugens, encodes four ILP genes (Nlilp1, Nlilp2, Nlilp3 and Nlilp4...

journal_title：Insect molecular biology

authors： Xue WH,Liu YL,Jiang YQ,He SF,Wang QQ,Yang ZN,Xu HJ

更新日期：2020-06-01 00:00:00

abstract：:Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...

journal_title：Insect molecular biology

authors： Gu J,Huang LX,Shen Y,Huang LH,Feng QL

更新日期：2012-10-01 00:00:00

abstract：:Transcriptional activity of nuclear receptors is the result of transactivation capability and the concentration of the receptor protein. The concentration of ecdysteroid receptor (EcR) isoforms, constitutively expressed in mammalian CHO cells, is dependent on a number of factors. As shown previously, ligand binding st...

journal_title：Insect molecular biology

authors： Schauer S,Burster T,Spindler-Barth M

更新日期：2012-06-01 00:00:00

abstract：:Vitellogenins, cyclorraphan yolk proteins and lepidopteran minor yolk proteins are three classes of female-specific proteins that serve as an embryonic nutritional store. Similarity to vertebrate lipid-binding proteins was established for vitellogenins and yolk proteins, vitellogenins being related to apolipoprotein B...

journal_title：Insect molecular biology

authors： Hens K,Lemey P,Macours N,Francis C,Huybrechts R

更新日期：2004-12-01 00:00:00

abstract：:Apis mellifera plays crucial roles in maintaining the balance of global ecosystems and stability of agricultural systems by helping pollination of flowering plants, including many crops. In recent years, this balance has been disrupted greatly by some pesticides, which results in great losses of honeybees worldwide. P...

journal_title：Insect molecular biology

authors： Hu YT,Tang CK,Wu CP,Wu PC,Yang EC,Tai CC,Wu YL

更新日期：2018-08-01 00:00:00

abstract：:Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates ...

journal_title：Insect molecular biology

authors： Lin Y,Liu H,Yang C,Gu J,Shen G,Zhang H,Chen E,Han C,Zhang Y,Xu Y,Wu J,Xia Q

更新日期：2017-10-01 00:00:00

abstract：:Little is known about when, how or even if the wing development gene network elucidated in Drosophila is deployed in direct-developing insects. Here we identify the wing development genes (as determined in Drosophila) of the pea aphid (Acyrthosiphon pisum), which produces winged or unwinged adults in response to envir...

journal_title：Insect molecular biology

authors： Brisson JA,Ishikawa A,Miura T

更新日期：2010-03-01 00:00:00

abstract：:Rapid degradation of odours after interaction with olfactory receptors is a critical step of the signal reception process. However, the implied mechanisms are still largely unknown in vertebrates as well as in insects. Involvement of odourant-degrading enzymes in odourant degradation within the antennae has been shown...

journal_title：Insect molecular biology

authors： Merlin C,Rosell G,Carot-Sans G,François MC,Bozzolan F,Pelletier J,Jacquin-Joly E,Guerrero A,Maïbèche-Coisne M

更新日期：2007-02-01 00:00:00