Gene transcription during exposure to, and recovery from, cold and desiccation stress in Drosophila melanogaster.

abstract：:Four cytochrome P450 genes, CYP6B8, CYP6B9, CYP6B27 and CYP6B28, exist in the Helicoverpa zea genome as two pairs of paralogs that evolved from gene duplication and 5'-polar gene conversion events. RT-PCR gel blot analyses have shown that all of these genes are expressed constitutively in midguts of all larval instars...

journal_title：Insect molecular biology

authors： Li X,Berenbaum MR,Schuler MA

更新日期：2002-08-01 00:00:00

abstract：:The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily of pheromone/odourant binding proteins. Although D7 proteins are among the most abundant salivary proteins in adult female mosquitoes and sand flies, their role in blood feeding remains elusive. In the present w...

journal_title：Insect molecular biology

authors： Valenzuela JG,Charlab R,Gonzalez EC,de Miranda-Santos IK,Marinotti O,Francischetti IM,Ribeiro JM

更新日期：2002-04-01 00:00:00

abstract：:The maternally inherited bacterium Wolbachia is well known for spreading in natural populations by manipulating the reproduction of its arthropod hosts, but can also have mutualist effects that increase host fitness. In mosquitoes and Drosophila some Wolbachia strains can lead to an increase in survival of virus-infec...

journal_title：Insect molecular biology

authors： Cattel J,Martinez J,Jiggins F,Mouton L,Gibert P

更新日期：2016-10-01 00:00:00

abstract：:We aimed to understand the underlying mechanism that regulates successively expressed cuticular protein (CP) genes around pupation in Bombyx mori. Quantitative PCR was conducted to clarify the expression profile of CP genes and ecdysone-responsive transcription factor (ERTF) genes around pupation. Ecdysone pulse treat...

journal_title：Insect molecular biology

authors： Shahin R,Iwanaga M,Kawasaki H

更新日期：2016-04-01 00:00:00

abstract：:We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...

journal_title：Insect molecular biology

authors： Chang WX,Gahan LJ,Tabashnik BE,Heckel DG

更新日期：1999-05-01 00:00:00

abstract：:Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associ...

journal_title：Insect molecular biology

authors： Ranson H,Paton MG,Jensen B,McCarroll L,Vaughan A,Hogan JR,Hemingway J,Collins FH

更新日期：2004-08-01 00:00:00

abstract：:Glutathione transferases (GSTs) are a diverse family of enzymes found ubiquitously in aerobic organisms. They play a central role in the detoxification of both endogenous and xenobiotic compounds and are also involved in intracellular transport, biosynthesis of hormones and protection against oxidative stress. Interes...

journal_title：Insect molecular biology

authors： Enayati AA,Ranson H,Hemingway J

更新日期：2005-01-01 00:00:00

abstract：:Haemolymph polypeptides from Plasmodium-refractory and -susceptible mosquitoes were compared by one- and two-dimensional gel electrophoresis. The refractory strain of Anopheles gambiae kills malaria parasites by a humoral melanization mechanism whereas the parasites develop normally in susceptible mosquitoes. The two ...

journal_title：Insect molecular biology

authors： Chun J,McMaster J,Han Y,Schwartz A,Paskewitz SM

更新日期：2000-02-01 00:00:00

abstract：:Two isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse, Glossina pallidipes and G. morsitans. These microorganisms appear similar to rickettsia-like organisms reported previously from various tsetse species. The 16S rRNA sequence analysis, however, pl...

journal_title：Insect molecular biology

authors： Beard CB,O'Neill SL,Mason P,Mandelco L,Woese CR,Tesh RB,Richards FF,Aksoy S

更新日期：1993-01-01 00:00:00

abstract：:A high number of (GT)n and (CT)n microsatellites were isolated from a partial genomic library of primitively eusocial wasp, Vespula rufa. On average, (GT)n and (CT)n repeats occur in the genome approximately every 8 kb and 2.5 kb respectively. This is the highest density of dinucleotide microsatellites reported in the...

journal_title：Insect molecular biology

authors： Thorén PA,Paxton RJ,Estoup A

更新日期：1995-08-01 00:00:00

abstract：:Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but ...

journal_title：Insect molecular biology

authors： Provost-Javier KN,Chen S,Rasgon JL

更新日期：2010-08-01 00:00:00

abstract：:Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...

journal_title：Insect molecular biology

authors： Yang F,Zhu B,Liu J,Liu Y,Jiang C,Sheng Q,Qiu J,Nie Z

更新日期：2020-02-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00

abstract：:Phenotypic diversity is frequently generated by differences in gene expression. In this study, we addressed the relationship between homology in gene expression and phenotype among four species of eusocial wasps. Specifically, we investigated the evolution of caste-specific and sex-specific gene expression patterns as...

journal_title：Insect molecular biology

authors： Hunt BG,Goodisman MA

更新日期：2010-10-01 00:00:00

abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...

journal_title：Insect molecular biology

authors： Horard B,Mangé A,Pélissier B,Couble P

更新日期：1994-11-01 00:00:00

abstract：:The insect digestive system is the first line of defence protecting cells and tissues of the body from a broad spectrum of toxins and antinutritional factors in its food. To gain insight into the nature and breadth of genes involved in adaptation to dietary challenge, a collection of 20 352 cDNAs was prepared from the...

journal_title：Insect molecular biology

authors： Chi YH,Salzman RA,Balfe S,Ahn JE,Sun W,Moon J,Yun DJ,Lee SY,Higgins TJ,Pittendrigh B,Murdock LL,Zhu-Salzman K

更新日期：2009-02-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...

journal_title：Insect molecular biology

authors： Gu J,Huang LX,Shen Y,Huang LH,Feng QL

更新日期：2012-10-01 00:00:00

abstract：:Some refractory anopheline mosquitoes are capable of killing Plasmodium, the causative agent of malaria, by melanotic encapsulation of invading ookinetes. Phenoloxidase (PO) appears to be involved in the formation of melanin and toxic metabolites in the surrounding capsule. A cDNA encoding Anopheles stephensi propheno...

journal_title：Insect molecular biology

authors： Cui L,Luckhart S,Rosenberg R

更新日期：2000-04-01 00:00:00

abstract：:Costelytra zealandica larvae are pests of New Zealand pastures causing damage by feeding on the roots of grasses and clovers. The major larval protein digestive enzymes are serine proteases (SPs), which are targets for disruption in pest control. An expressed sequence tag (EST) library from healthy, third instar larva...

journal_title：Insect molecular biology

authors： Marshall SD,Gatehouse LN,Becher SA,Christeller JT,Gatehouse HS,Hurst MR,Boucias DG,Jackson TA

更新日期：2008-06-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormon...

journal_title：Insect molecular biology

authors： Lestradet M,Gervasio E,Fraichard S,Dupas S,Alabouvette J,Lemoine A,Charles JP

更新日期：2009-08-01 00:00:00

abstract：:The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST of Bombyx mori (bmGSTO) was shown to be induced after exposure to various environmental stresses. A soluble form of recombinant protein (rbmGSTO) was functionally ...

journal_title：Insect molecular biology

authors： Yamamoto K,Teshiba S,Shigeoka Y,Aso Y,Banno Y,Fujiki T,Katakura Y

更新日期：2011-06-01 00:00:00

abstract：:The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...

journal_title：Insect molecular biology

authors： Reim T,Scheiner R

更新日期：2014-12-01 00:00:00

abstract：:Lucilia sericata maggots are used world-wide in biosurgery for the medical treatment of nonhealing wounds because they ingest necrotic tissues and significantly promote healing. To gain further insight into interdependencies between ecological adaptation and molecular evolution of innate immunity in Diptera, we used t...

journal_title：Insect molecular biology

authors： Altincicek B,Vilcinskas A

更新日期：2009-02-01 00:00:00

abstract：:In fat body of the mosquito, Aedes aegypti, a cycle of ribosome accumulation and degradation accompanies synthesis of the yolk protein precursor, vitellogenin. Here we compare the transcription and translation of ribosomal proteins rpS6, rpL8 and rpL34, relative to rRNA and vitellogenin genes in Aedes aegypti fat body...

journal_title：Insect molecular biology

authors： Niu LL,Fallon AM

更新日期：2000-12-01 00:00:00

abstract：:Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDN...

journal_title：Insect molecular biology

authors： Gaines PJ,Sampson CM,Rushlow KE,Stiegler GL

更新日期：1999-02-01 00:00:00

abstract：:Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicator...

journal_title：Insect molecular biology

authors： Thibault ST,Luu HT,Vann N,Miller TA

更新日期：1999-02-01 00:00:00

abstract：:Phenoloxidases, including tyrosinases and laccases, are enzymes involved in the synthesis of melanin, a process that can be elicited during insect immune responses, cuticle maturation, wound healing and egg chorion development. We cloned a putative inhibitor of melanization (POI) from Anopheles gambiae on the basis of...

journal_title：Insect molecular biology

authors： Shi L,Li B,Paskewitz SM

更新日期：2006-06-01 00:00:00

abstract：:In this paper we describe the peculiar structures and preferential codon usage found in wild silkworm fibroin genes. We determined a 1350 bp nucleotide sequence from the Chinese oak silkworm, Antheraea pernyi. The deduced amino acid sequence was partitioned into thirteen polyalanine-containing repetitive motifs, which...

journal_title：Insect molecular biology

authors： Yukuhiro K,Kanda T,Tamura T

更新日期：1997-02-01 00:00:00