The cis-regulatory sequences required for expression of the Drosophila melanogaster adult cuticle gene ACP65A.

Abstract:

:Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormone action at the molecular level. The Drosophila ACP65A cuticle gene is expressed exclusively during the synthesis of the adult exoskeleton, in epidermal domains synthesising flexible cuticle. We have examined the cis-regulatory sequences of ACP65A using phylogenetic comparisons and functional analysis, and find that only about 180 bp are essential, including an 81 bp intron. The restriction of ACP65A expression appears to depend on a strong repression mechanism.

journal_name

Insect Mol Biol

journal_title

Insect molecular biology

authors

Lestradet M,Gervasio E,Fraichard S,Dupas S,Alabouvette J,Lemoine A,Charles JP

doi

10.1111/j.1365-2583.2009.00893.x

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

431-41

issue

4

eissn

0962-1075

issn

1365-2583

pii

IMB893

journal_volume

18

pub_type

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