Abstract：
:In fat body of the mosquito, Aedes aegypti, a cycle of ribosome accumulation and degradation accompanies synthesis of the yolk protein precursor, vitellogenin. Here we compare the transcription and translation of ribosomal proteins rpS6, rpL8 and rpL34, relative to rRNA and vitellogenin genes in Aedes aegypti fat body after eclosion, and in response to a blood meal. Analysis using Northern blots and reverse-transcription polymerase chain reactions (RT-PCR) showed that the rpS6, rpL8 and rpL34 genes are coordinately regulated with respect to one another, and that ribosomal protein gene expression in this system was predominantly regulated by transcription during the 3-4 days between adult eclosion and blood feeding. After a blood meal, ribosomal protein mRNA levels remained similar to those in unfed females during the first 18 h, then declined to minimum levels by 48 h after the blood meal. These data indicate that transcription of ribosomal protein genes is low in vitellogenic mosquitoes, relative to previtellogenic females. Experiments with the dissected fat body, however, showed that levels of acetic acid-soluble proteins increased by approximately threefold between 12 and 24 h after the blood meal. Taken together, these observations suggest that the active translation of ribosomal proteins from stable mRNA accompanies ribosome biosynthesis after the blood meal. Thus, in the fat body of adult female mosquitoes, the expression of ribosomal protein genes is regulated at the level of transcription before the blood meal, while translational control is the predominant regulatory mechanism after the blood meal.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Niu LL,Fallon AMdoi
10.1046/j.1365-2583.2000.00226.xsubject
Has Abstractpub_date
2000-12-01 00:00:00pages
613-23issue
6eissn
0962-1075issn
1365-2583pii
imb226journal_volume
9pub_type
杂志文章abstract：:Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00284.x
更新日期：2001-10-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00567.x
更新日期：2005-08-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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更新日期：1995-08-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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更新日期：1997-05-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12495
更新日期：2018-10-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：2019-12-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12205
更新日期：2016-04-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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更新日期：2000-06-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：2007-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00304.x
更新日期：2002-02-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2003.00410.x
更新日期：2003-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1365-2583.2005.00602.x
更新日期：2005-12-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.01037.x
更新日期：2010-10-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00204.x
更新日期：2000-08-01 00:00:00
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journal_title：Insect molecular biology
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doi：10.1111/imb.12096
更新日期：2014-08-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2007.00737.x
更新日期：2007-08-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2000.00157.x
更新日期：2000-02-01 00:00:00
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journal_title：Insect molecular biology
pub_type： 杂志文章
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journal_title：Insect molecular biology
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更新日期：2007-08-01 00:00:00
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更新日期：2011-08-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：1999-05-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：2007-04-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：2005-04-01 00:00:00
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journal_title：Insect molecular biology
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更新日期：2008-04-01 00:00:00
abstract：:The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-ty...
journal_title：Insect molecular biology
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更新日期：2019-12-01 00:00:00
