Abstract：
:A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis (Bt). The full-length cDNA (5371 bp in length) encodes an open reading frame for a 1688 amino acid polypeptide. The putative protein has similar architecture to cadherin-like proteins isolated from lepidopteran midguts that have been shown to bind to Cry1 Bt toxins and have been implicated in Bt resistance. The D. v. virgifera cadherin-like gene is expressed primarily in the larval midgut and regulated during development, with high levels of expression observed in all instars and adults but not pupae. The corresponding genomic sequence spans more than 90 kb and is interspersed with 30 large introns. The genomic organization of the cadherin-like gene for this coleopteran species bears strong resemblance to lepidopteran cadherins suggesting a common molecular basis for susceptibility to Cry3 toxins in Coleoptera.
journal_name
Insect Mol Bioljournal_title
Insect molecular biologyauthors
Sayed A,Nekl ER,Siqueira HA,Wang HC,Ffrench-Constant RH,Bagley M,Siegfried BDdoi
10.1111/j.1365-2583.2007.00755.xsubject
Has Abstractpub_date
2007-10-01 00:00:00pages
591-600issue
5eissn
0962-1075issn
1365-2583pii
IMB755journal_volume
16pub_type
杂志文章abstract：:Insect chitin synthase is an essential enzyme involved in chitin biosynthesis in insects. Chitin synthase A (CHSA) is expressed in different insect tissues during different developmental stages. CHSA contains alternative-splicing exons that allow tissue- and development-specific chitin synthesis. Here, we report that ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2012.01145.x
更新日期：2012-08-01 00:00:00
abstract：:The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2002.00373.x
更新日期：2002-12-01 00:00:00
abstract：:The honey bee (Apis mellifera L.) has developed into an important ethological model organism for social behaviour and behavioural plasticity. Bees perform a complex age-dependent division of labour with the most pronounced behavioural differences occurring between in-hive bees and foragers. Whereas nurse bees, for exa...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12130
更新日期：2014-12-01 00:00:00
abstract：:Previous research has led to the idea that derived traits can arise through the evolution of novel roles for conserved genes. We explored whether neuropeptide Y (NPY)-like signalling, a conserved pathway that regulates food-related behaviour, is involved in a derived, nutritionally-related trait, the division of labou...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2011.01068.x
更新日期：2011-06-01 00:00:00
abstract：:We aimed to understand the underlying mechanism that regulates successively expressed cuticular protein (CP) genes around pupation in Bombyx mori. Quantitative PCR was conducted to clarify the expression profile of CP genes and ecdysone-responsive transcription factor (ERTF) genes around pupation. Ecdysone pulse treat...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12207
更新日期：2016-04-01 00:00:00
abstract：:Fast and reliable methods for assessing the mobility of the transposable element Minos have been developed. These methods are based on the detection of excision and insertion of Minos transposons from and into plasmids which are co-introduced into cells. Excision is detected by polymerase chain reaction (PCR) with app...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00183.x
更新日期：2000-06-01 00:00:00
abstract：:Post-embryonic development in insects requires successive molts. Molts are triggered by ecdysteroids, and the nature of the molt (larval, pupal or adult) is determined by juvenile hormones. The genes encoding cuticle proteins are targets of both classes of hormones, and therefore are interesting models to study hormon...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00893.x
更新日期：2009-08-01 00:00:00
abstract：:Juvenile hormone esterases (JHEs) are required for the degradation of juvenile hormones (JHs) in insects. Here, we report the cloning and analysis of the jhe gene in the red flour beetle, Tribolium castaneum, a model insect of Coleoptera. The Tcjhe gene was strongly expressed at the final instar larva, as would be exp...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2010.01019.x
更新日期：2010-08-01 00:00:00
abstract：:Toll and related proteins play important roles in innate immunity in both invertebrates and vertebrates. In Drosophila melanogaster, Tehao shares a striking similarity in its intracellular domain with Toll. In this paper, we show that Tehao is expressed throughout development and appears to be glycosylated. In transie...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00284.x
更新日期：2001-10-01 00:00:00
abstract：:Culex quinquefasciatus is a vector of many diseases that adversely impact human and animal health; however, compared to other mosquito vectors limited genome engineering technologies have been characterized for this vector. Clustered regularly interspaced short palindrome repeats-associated protein 9 (CRISPR-Cas9) bas...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12626
更新日期：2020-04-01 00:00:00
abstract：:In Culex pipiens mosquitoes, A2 esterase alleles are co-amplified with B2 esterase alleles in response to selection with organophosphate insecticides. In this study the amplified A2 and B2 sequences were compared between twelve strains from four continents by restriction mapping. The restriction maps were almost ident...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1998.00077.x
更新日期：1998-08-01 00:00:00
abstract：:The tobacco hornworm Manduca sexta is an important model for insect physiology but genomic and transcriptomic data are currently lacking. Following a recent pyrosequencing study generating immune related expressed sequence tags (ESTs), here we use this new technology to define the M. sexta larval midgut transcriptome....
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00936.x
更新日期：2010-02-01 00:00:00
abstract：:A homologue of the Drosophila melanogaster eye-colour gene, scarlet (st), has been isolated from the genome of the tephritid fruit fly, Bactrocera tryoni. The comparison of the B. tryoni and D. melanogaster scarlet gene shows 71.2% and 79.3% sequence identity at the DNA and the derived amino acid level, respectively. ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2003.00410.x
更新日期：2003-06-01 00:00:00
abstract：:Serine protease gene fragments approximately 480 nucleotides in length were amplified from Ctenocephalides felis larval and adult cDNA libraries using degenerate oligonucleotide PCR primers. Partial clones of thirty-eight distinct serine protease encoding sequences were isolated, and nineteen different full-length cDN...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1999.810011.x
更新日期：1999-02-01 00:00:00
abstract：:In fat body of the mosquito, Aedes aegypti, a cycle of ribosome accumulation and degradation accompanies synthesis of the yolk protein precursor, vitellogenin. Here we compare the transcription and translation of ribosomal proteins rpS6, rpL8 and rpL34, relative to rRNA and vitellogenin genes in Aedes aegypti fat body...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2000.00226.x
更新日期：2000-12-01 00:00:00
abstract：:The pea aphid (Acyrthosiphon pisum) is the first whole genome sequenced insect with a hemimetabolic development and an emerging model organism for studies in ecology, evolution and development. The insect steroid moulting hormone 20-hydroxyecdysone (20E) controls and coordinates development in insects, especially the ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00957.x
更新日期：2010-03-01 00:00:00
abstract：:Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2012.01158.x
更新日期：2012-10-01 00:00:00
abstract：:Acetylation is an important, reversible posttranslational modification to a protein. In a previous study, we found that there were a large number of acetylated sites in various nutrient storage proteins of the silkworm haemolymph. In this study, we confirmed that acetylation can affect the stability of nutrient storag...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12613
更新日期：2020-02-01 00:00:00
abstract：:Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional t...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.0962-1075.2001.00275.x
更新日期：2001-08-01 00:00:00
abstract：:Culicoides spp. are vectors of several infectious diseases of veterinary importance and a major cause of allergy in horses and other livestock. Their saliva contains a number of proteins which enable blood feeding, enhance disease transmission and act as allergens. We report the construction of a novel cDNA library fr...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2009.00882.x
更新日期：2009-06-01 00:00:00
abstract：:Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2006.00674.x
更新日期：2006-10-01 00:00:00
abstract：:Culex pipiens quinquefasciatus Say and Culex pipiens pipiens Linnaeus are sibling species incriminated as important vectors of emerging and re-emerging infectious diseases worldwide. The two forms differ little morphologically and are differentiated mainly based upon ecological, behavioural, physiological and genetic ...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.2006.00707.x
更新日期：2007-04-01 00:00:00
abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00175.x
更新日期：1994-11-01 00:00:00
abstract：:The phylogeny of the cryptic species complex of wasps in the genus Nasonia was inferred by analysis of nucleotide sequences of an rDNA internal transcribed spacer (ITS2) and the D2 region of 28S rDNA. Phylogenetic analysis showed that N. vitripennis descended from a theoretical common ancestor with that of a lineage t...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/j.1365-2583.1994.tb00142.x
更新日期：1993-01-01 00:00:00
abstract：:The Fanconi anaemia (FA) pathway is responsible for interstrand crosslink (ICL) repair. Among the FA core complex components, FANCM is believed to act as a damage sensor for the ICL-blocked replication fork and also as a molecular platform for FA core complex assembly and interaction with Bloom's syndrome (BS) complex...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12072
更新日期：2014-04-01 00:00:00
abstract：:Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.2002.00328.x
更新日期：2002-06-01 00:00:00
abstract：:The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1997.00151.x
更新日期：1997-02-01 00:00:00
abstract：:Sensory neurone membrane proteins (SNMPs) function as essential cofactors for insect sex pheromone detection. In this study, we report two SNMPs in Ectropis obliqua Prout, a serious geometrid pest that produces typical Type-II sex pheromones. Sequence alignments and phylogenetic analyses showed that EoblSNMP1 and Eobl...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12555
更新日期：2019-06-01 00:00:00
abstract：:We screened a midgut cDNA library from diamondback moth, Plutella xylostella, with a probe generated using sequence information from an aminopeptidase N gene from Manduca sexta (MsAPN-1). The sequence recovered (PxAPN-A) encodes a protein of 988 resides with a 60% sequence identity to MsAPN-1. The two proteins share a...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1046/j.1365-2583.1999.820171.x
更新日期：1999-05-01 00:00:00
abstract：:Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present stu...
journal_title：Insect molecular biology
pub_type： 杂志文章
doi：10.1111/imb.12532
更新日期：2019-02-01 00:00:00
