Characterization and expression of the odorant-binding protein 7 gene in Anopheles stephensi and comparative analysis among five mosquito species.

abstract：:Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae, we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding fu...

journal_title：Insect molecular biology

authors： Ranson H,Nikou D,Hutchinson M,Wang X,Roth CW,Hemingway J,Collins FH

更新日期：2002-10-01 00:00:00

abstract：:A cadherin-like gene associated with larval midgut tissues was cloned from western corn rootworm (Diabrotica virgifera virgifera: Coleoptera), an economically important agricultural pest in North America and Europe and the primary target pest species for corn hybrids expressing Cry3 toxins from Bacillus thuringiensis ...

journal_title：Insect molecular biology

authors： Sayed A,Nekl ER,Siqueira HA,Wang HC,Ffrench-Constant RH,Bagley M,Siegfried BD

更新日期：2007-10-01 00:00:00

abstract：:We identified two different groups of piggyBac-like elements (PLE) in the tobacco budworm, Heliothis virescens, and named them HvPLE1 and HvPLE2. An intact copy of HvPLE1 revealed the characteristics of PLE: inverted terminal repeats, inverted subterminal repeats, and an open reading frame encoding transposase, wherea...

journal_title：Insect molecular biology

authors： Wang J,Ren X,Miller TA,Park Y

更新日期：2006-08-01 00:00:00

abstract：:Small RNA regulatory pathways are used to control the activity of transposons, regulate gene expression and resist infecting viruses. We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito Aedes aegypti. Under standard conditions, mRNA-derived endo-siR...

journal_title：Insect molecular biology

authors： Adelman ZN,Anderson MA,Liu M,Zhang L,Myles KM

更新日期：2012-06-01 00:00:00

abstract：:Insect chitinases are hydrolytic enzymes required for the degradation of chitin. They are essential for insect moulting and metamorphosis. In this study, the regulation mechanism of a chitinase gene, Bombyx mori chitinase 5 (BmCHT5), was studied. Quantitative reverse transcription PCR (qRT-PCR) analysis showed that Bm...

journal_title：Insect molecular biology

authors： Zhang X,Zheng S

更新日期：2017-04-01 00:00:00

abstract：:A large and diverse family of serine protease genes was identified in first-instar larval cDNA of the sheep blowfly (Lucilia cuprina). This complex repertoire of genes was identified via a PCR approach using highly degenerate primers based on structurally conserved regions which surround the active site His and Ser re...

journal_title：Insect molecular biology

authors： Elvin CM,Vuocolo T,Smith WJ,Eisemann CH,Riddles PW

更新日期：1994-05-01 00:00:00

abstract：:Three genes encoding clan MB metalloproteinases (EpMP1-3) were identified from venom glands of the ectoparasitic wasp Eulophus pennicornis. The derived amino acid sequences predict mature proteins of approximately 46 kDa, with a novel two-domain structure comprising a C-terminal reprolysin domain, and an N-terminal do...

journal_title：Insect molecular biology

authors： Price DR,Bell HA,Hinchliffe G,Fitches E,Weaver R,Gatehouse JA

更新日期：2009-04-01 00:00:00

abstract：:Microsatellites are important molecular markers used in numerous genetic contexts. Despite this widespread use, the evolutionary processes governing microsatellite distribution and diversity remain controversial. Here, we present results on the distribution of microsatellites of three species in the parasitic wasp gen...

journal_title：Insect molecular biology

authors： Pannebakker BA,Niehuis O,Hedley A,Gadau J,Shuker DM

更新日期：2010-02-01 00:00:00

abstract：:Sperm usage was investigated in an instrumentally inseminated honeybee queen. Her progeny were examined in the first 3 months of the egg-laying period using a microsatellite marker. Frequencies of different subfamilies differed significantly from one month to another. However, there was no evidence for sperm displacem...

journal_title：Insect molecular biology

authors： Franck P,Coussy H,Le Conte Y,Solignac M,Garnery L,Cornuet JM

更新日期：1999-08-01 00:00:00

abstract：:Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupa...

journal_title：Insect molecular biology

authors： Gu J,Huang LX,Shen Y,Huang LH,Feng QL

更新日期：2012-10-01 00:00:00

abstract：:In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS-treated flies and were labelled as immune response prot...

journal_title：Insect molecular biology

authors： Schlenke TA,McKean KA

更新日期：2005-04-01 00:00:00

abstract：:Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. T...

journal_title：Insect molecular biology

authors： Hasselmann M,Fondrk MK,Page RE,Beye M

更新日期：2001-12-01 00:00:00

abstract：:We have used a PCR assay to screen 404 insects and related arthropods for mariner elements using primers corresponding to amino acids conserved between the mariner elements of Drosophila mauritiana and a moth, Hyalophora cecropia. Potential mariner elements were detected in sixty-three species, representing ten insect...

journal_title：Insect molecular biology

authors： Robertson HM,MacLeod EG

更新日期：1993-01-01 00:00:00

abstract：:The ribosomal DNA cistron from the large raspberry aphid, Amphorophora idaei (Hemiptera: Aphididae), has been mapped by restriction analysis. The results showed that the map of A. idaei was similar to those of the previously characterized aphids Schizaphis graminum and Acyrthosiphon pisum. An extra Bgl II site was fou...

journal_title：Insect molecular biology

authors： Fenton B,Birch AN,Malloch G,Woodford JA,Gonzalez C

更新日期：1994-08-01 00:00:00

abstract：:PCR primers designed from the alpha-esterase gene cluster of Drosophila melanogaster have been used to isolate fragments from eight esterase genes in the Australian sheep blowfly, Lucilia cuprina. Phylogenetic analysis suggests that three are homologues of the alpha E7, alpha E8 and alpha E9 genes of the alpha-esteras...

journal_title：Insect molecular biology

authors： Newcomb RD,East PD,Russell RJ,Oakeshott JG

更新日期：1996-08-01 00:00:00

abstract：:The products of the maternal-effect genes, nanos (nos) and oskar (osk), are important for the development of germ cells in insects. Furthermore, these genes have been proposed as candidates for donating functional DNA regulatory sequences for use in gene drive systems to control transmission of mosquito-borne pathogen...

journal_title：Insect molecular biology

authors： Juhn J,Marinotti O,Calvo E,James AA

更新日期：2008-09-01 00:00:00

abstract：:RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA ...

journal_title：Insect molecular biology

authors： Thairu MW,Skidmore IH,Bansal R,Nováková E,Hansen TE,Li-Byarlay H,Wickline SA,Hansen AK

更新日期：2017-06-01 00:00:00

abstract：:Many hemimetabolous insects produce their own cellulase enzymes from the glycoside hydrolase family 9, first observed in termites and cockroaches. Phasmatodea have multiple cellulases, some of which are multifunctional and can degrade xylan or xyloglucan. To discover when these abilities evolved, we identified cellula...

journal_title：Insect molecular biology

authors： Shelomi M,Wipfler B,Zhou X,Pauchet Y

更新日期：2020-02-01 00:00:00

abstract：:We previously identified a novel non-coding immediate early gene, termed kakusei , from the honeybee (Apis mellifera) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al .,2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in...

journal_title：Insect molecular biology

authors： Kiya T,Kunieda T,Kubo T

更新日期：2008-09-01 00:00:00

abstract：:Neuropeptides and their G protein-coupled receptors are widespread throughout Metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. One such neuropeptide is the insect adipokinetic hormone (AKH), which is related to the mammalian gonadotropin-releasing hormone. AKH ha...

journal_title：Insect molecular biology

authors： Zandawala M,Hamoudi Z,Lange AB,Orchard I

更新日期：2015-04-01 00:00:00

abstract：:The full-length Hsp90 cDNA in Delia antiqua was cloned and sequenced. The deduced polypeptide comprised 717 amino acid residues, with a molecular mass of 82 140 Da. Summer- and winter-diapauses both elevated HSP90 transcript levels in D. antiqua pupae. Levels gradually increased with time in summer diapausing pupae wh...

journal_title：Insect molecular biology

authors： Chen B,Kayukawa T,Monteiro A,Ishikawa Y

更新日期：2005-12-01 00:00:00

abstract：:The intensely studied white gene is widely used as a genetic marker in Drosophila melanogaster. Here, we cloned and characterized the white gene in an important pest of the fruit industry, Bactrocera dorsalis, to understand its functional role in pigmentation. We obtained BdWhite knockout strains, based on the wild-ty...

journal_title：Insect molecular biology

authors： Bai X,Zeng T,Ni XY,Su HA,Huang J,Ye GY,Lu YY,Qi YX

更新日期：2019-12-01 00:00:00

abstract：:In most, if not all non-Drosophila insect systems, the study of gene regulation relies on in vitro analyses. With the aim of providing an in vivo assay, we developed an original protocol using the Bombyx mori silk gland, by which the functioning of cloned gene promoters can be efficiently measured. This consists of in...

journal_title：Insect molecular biology

authors： Horard B,Mangé A,Pélissier B,Couble P

更新日期：1994-11-01 00:00:00

abstract：:Molecular mechanisms underlying the interaction between malarial sporozoites and putative receptor(s) on the salivary glands of Anopheles gambiae remain largely unknown. In previous studies, a salivary gland protein of ~100 kDa was identified as a putative target based on recognition of the protein by a monoclonal ant...

journal_title：Insect molecular biology

authors： Okulate MA,Kalume DE,Reddy R,Kristiansen T,Bhattacharyya M,Chaerkady R,Pandey A,Kumar N

更新日期：2007-12-01 00:00:00

abstract：:Age-dependent division of labour in honeybees was shown to be connected to sensory response thresholds. Foragers show a higher gustatory responsiveness than nurse bees. It is generally assumed that nutrition-related signalling pathways underlie this behavioural plasticity. Here, one important candidate gene is the for...

journal_title：Insect molecular biology

authors： Thamm M,Sturm K,Schlossmann J,Scheiner R

更新日期：2018-12-01 00:00:00

abstract：:The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adu...

journal_title：Insect molecular biology

authors： Venancio TM,Cristofoletti PT,Ferreira C,Verjovski-Almeida S,Terra WR

更新日期：2009-02-01 00:00:00

abstract：:We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expresse...

journal_title：Insect molecular biology

authors： Yamamoto-Kihara M,Kotani E

更新日期：2004-04-01 00:00:00

abstract：:The bacterium Bacillus thuringiensis synthesizes toxins (delta-endotoxins) that are highly specific for insects. Once ingested, the activated form of the toxin binds to a specific receptor(s) located on the midgut epithelial cells, inserts into the membrane causing the formation of leakage pores and eventual death of ...

journal_title：Insect molecular biology

authors： Gill M,Ellar D

更新日期：2002-12-01 00:00:00

abstract：:Injection of double-stranded RNA (dsRNA) corresponding to the silkworm white gene (Bmwh3) into preblastoderm eggs of the wild-type silkworm induced phenotypes similar to those observed with mutants of the white egg 3 locus (10-19.6). The induced phenotypes were characterized by the presence of white eggs and transluce...

journal_title：Insect molecular biology

authors： Quan GX,Kanda T,Tamura T

更新日期：2002-06-01 00:00:00

abstract：:We isolated multiple copies of mariner-like element (MLE) from the pink bollworm (PBW), Pectinophora gossypiella, a key lepidopteran cotton pest. Although all the MLE sequences contain multiple mutations accumulated in their transposase coding region, the consensus sequence revealed a putative ancestral transposase en...

journal_title：Insect molecular biology

authors： Wang J,Staten R,Miller TA,Park Y

更新日期：2005-10-01 00:00:00