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A yeast intronic splicing enhancer and Nam8p are required for Mer1p-activated splicing.

Abstract：

:Three introns whose splicing is activated during meiosis in S. cerevisiae contain a Mer1p-dependent splicing enhancer. The enhancer can impose Mer1p-activated splicing upon the constitutively spliced actin intron provided the basal splicing efficiency of actin is first reduced. Of several nonessential splicing factors tested, only the U1 snRNP protein Nam8p is indispensable for Mer1 p-activated splicing. We show that Mer1p associates with the U1 snRNP even in the absence of Nam8p or pre-mRNA. This work defines a yeast splicing enhancer and shows that constitutively expressed and cell type-specific factors combine to regulate splicing of a specific subset of pre-mRNAs including SPO70, MER2, and MER3.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Spingola M,Ares M Jr

doi

10.1016/s1097-2765(00)00033-2

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

329-38

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(00)00033-2

journal_volume

6

pub_type

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