Abstract:
:One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially with loss of a conserved Psi in the A site of tRNA binding. Depletion of other Psis had subtle or no apparent effect on activity; however, synergistic effects were observed in some combinations. Pseudouridines are proposed to enhance ribosome activity by altering rRNA folding and interactions, with some Psis having greater effects than others. The possibility that modifying snoRNPs might affect ribosome structure in other ways is also discussed.
journal_name
Mol Celljournal_title
Molecular cellauthors
King TH,Liu B,McCully RR,Fournier MJdoi
10.1016/s1097-2765(03)00040-6subject
Has Abstractpub_date
2003-02-01 00:00:00pages
425-35issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(03)00040-6journal_volume
11pub_type
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