The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation.


:The mRNA cap structure is bound by either the nuclear (CBC) or the cytoplasmic (eIF4F) cap binding complex. Following mRNA export, CBC must be exchanged for eIF4F in the cytoplasm. It is not known how this exchange occurs or how this RNP remodeling event is integrated with mRNA function. Here we report genetic and biochemical evidence that the yeast translation initiation factor eIF4G associates with CBC, and that eIF4E, the eIF4F component that binds both the cap and eIF4G, antagonizes this interaction. Furthermore, we find that CBC can stimulate translation in extracts containing an eIF4G protein deficient for eIF4E binding. These data suggest that eIF4E binding to the eIF4G-CBC complex on newly exported mRNA displaces CBC, and that the first round of translation on mRNA may occur via a different mechanism than subsequent rounds.


Mol Cell


Molecular cell


Fortes P,Inada T,Preiss T,Hentze MW,Mattaj IW,Sachs AB


Has Abstract


2000-07-01 00:00:00














  • Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains.

    abstract::The functional significance of mono-, di-, and trimethylation of lysine residues within histone proteins remains unclear. Antibodies developed to selectively recognize each of these methylated states at histone H3 lysine 9 (H3 Lys9) demonstrated that mono- and dimethylation localized specifically to silent domains wit...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Rice JC,Briggs SD,Ueberheide B,Barber CM,Shabanowitz J,Hunt DF,Shinkai Y,Allis CD

    更新日期:2003-12-01 00:00:00

  • Progesterone receptor contains a proline-rich motif that directly interacts with SH3 domains and activates c-Src family tyrosine kinases.

    abstract::Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

    更新日期:2001-08-01 00:00:00

  • Condensin: The Little Motor Protein that Could.

    abstract::Kong et al. (2020) present the low-resolution structure of the ATPɣS-bound human condensin I and II complexes and demonstrate that human condensins can extrude DNA loops in a symmetric and asymmetric fashion and compact nucleosome-bound DNA. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章


    authors: Goloborodko A

    更新日期:2020-07-02 00:00:00

  • EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency.

    abstract::Trimethylation on H3K27 (H3K27me3) mediated by Polycomb repressive complex 2 (PRC2) has been linked to embryonic stem cell (ESC) identity and pluripotency. EZH2, the catalytic subunit of PRC2, has been reported as the sole histone methyltransferase that methylates H3K27 and mediates transcriptional silencing. Analysis...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Shen X,Liu Y,Hsu YJ,Fujiwara Y,Kim J,Mao X,Yuan GC,Orkin SH

    更新日期:2008-11-21 00:00:00

  • ChIC and ChEC; genomic mapping of chromatin proteins.

    abstract::To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Schmid M,Durussel T,Laemmli UK

    更新日期:2004-10-08 00:00:00

  • A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Goulet B,Baruch A,Moon NS,Poirier M,Sansregret LL,Erickson A,Bogyo M,Nepveu A

    更新日期:2004-04-23 00:00:00

  • Crystal structure of the vesicular transport protein Sec17: implications for SNAP function in SNARE complex disassembly.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Rice LM,Brunger AT

    更新日期:1999-07-01 00:00:00

  • Structure and mechanism of RNA polymerase II CTD phosphatases.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Kamenski T,Heilmeier S,Meinhart A,Cramer P

    更新日期:2004-08-13 00:00:00

  • Reconstitution of an Argonaute-dependent small RNA biogenesis pathway reveals a handover mechanism involving the RNA exosome and the exonuclease QIP.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Xue Z,Yuan H,Guo J,Liu Y

    更新日期:2012-05-11 00:00:00

  • Dicer-TRBP complex formation ensures accurate mammalian microRNA biogenesis.

    abstract::RNA-mediated gene silencing in human cells requires the accurate generation of ∼22 nt microRNAs (miRNAs) from double-stranded RNA substrates by the endonuclease Dicer. Although the phylogenetically conserved RNA-binding proteins TRBP and PACT are known to contribute to this process, their mode of Dicer binding and the...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Wilson RC,Tambe A,Kidwell MA,Noland CL,Schneider CP,Doudna JA

    更新日期:2015-02-05 00:00:00

  • RNA binding of PRC2: promiscuous or well ordered?

    abstract::The polycomb repressive complex 2 (PRC2) methylates histones for epigenetic silencing and associates with thousands of protein-coding and noncoding RNAs. How the recruitment of PRC2 to specific sites is facilitated is currently unclear. Two recent studies have deciphered the impact of RNA binding on PRC2 recruitment a...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章


    authors: Kretz M,Meister G

    更新日期:2014-07-17 00:00:00

  • Transcription and processing of human microRNA precursors.

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    journal_title:Molecular cell

    pub_type: 杂志文章,评审


    authors: Cullen BR

    更新日期:2004-12-22 00:00:00

  • The actin-bundling protein palladin is an Akt1-specific substrate that regulates breast cancer cell migration.

    abstract::The phosphatidylinositol 3-kinase (PI3K) signaling pathway is frequently deregulated in cancer. Downstream of PI3K, Akt1 and Akt2 have opposing roles in breast cancer invasive migration, leading to metastatic dissemination. Here, we identify palladin, an actin-associated protein, as an Akt1-specific substrate that mod...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Chin YR,Toker A

    更新日期:2010-05-14 00:00:00

  • Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin.

    abstract::Pilin assembly into type IV pili is required for virulence by bacterial pathogens that cause diseases such as cholera, pneumonia, gonorrhea, and meningitis. Crystal structures of soluble, N-terminally truncated pilin from Vibrio cholera toxin-coregulated pilus (TCP) and full-length PAK pilin from Pseudomonas aeruginos...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Craig L,Taylor RK,Pique ME,Adair BD,Arvai AS,Singh M,Lloyd SJ,Shin DS,Getzoff ED,Yeager M,Forest KT,Tainer JA

    更新日期:2003-05-01 00:00:00

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Shen X,Ranallo R,Choi E,Wu C

    更新日期:2003-07-01 00:00:00

  • METTL3 Gains R/W Access to the Epitranscriptome.

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    journal_title:Molecular cell

    pub_type: 评论,杂志文章


    authors: Schumann U,Shafik A,Preiss T

    更新日期:2016-05-05 00:00:00

  • Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.

    abstract::Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putativ...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Kong M,Cutts EE,Pan D,Beuron F,Kaliyappan T,Xue C,Morris EP,Musacchio A,Vannini A,Greene EC

    更新日期:2020-07-02 00:00:00

  • Epigenetic instability due to defective replication of structured DNA.

    abstract::The accurate propagation of histone marks during chromosomal replication is proposed to rely on the tight coupling of replication with the recycling of parental histones to the daughter strands. Here, we show in the avian cell line DT40 that REV1, a key regulator of DNA translesion synthesis at the replication fork, i...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Sarkies P,Reams C,Simpson LJ,Sale JE

    更新日期:2010-12-10 00:00:00

  • Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive feedback loop.

    abstract::Caspase-2 is an evolutionarily conserved caspase, yet its biological function and cleavage targets are poorly understood. Caspase-2 is activated by the p53 target gene product PIDD (also known as LRDD) in a complex called the Caspase-2-PIDDosome. We show that PIDD expression promotes growth arrest and chemotherapy res...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Oliver TG,Meylan E,Chang GP,Xue W,Burke JR,Humpton TJ,Hubbard D,Bhutkar A,Jacks T

    更新日期:2011-07-08 00:00:00

  • Dynamic RNA modifications in posttranscriptional regulation.

    abstract::Cellular RNAs can be chemically modified over a hundred different ways. These modifications were once thought to be static, discrete, and utilized to fine-tune RNA structure and function. However, recent studies have revealed that some modifications, like mRNA methylation, can be reversed, and these reversible modific...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审


    authors: Wang X,He C

    更新日期:2014-10-02 00:00:00

  • ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals.

    abstract::Animal cells have a remarkable capacity to adopt durable and heritable gene expression programs or epigenetic states that define the physical properties and diversity of somatic cell types. The maintenance of epigenetic programs depends on poorly understood pathways that prevent gain or loss of inherited signals. In t...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Ishidate T,Ozturk AR,Durning DJ,Sharma R,Shen EZ,Chen H,Seth M,Shirayama M,Mello CC

    更新日期:2018-05-17 00:00:00

  • Dynamics of enhancer-promoter communication during differentiation-induced gene activation.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Hatzis P,Talianidis I

    更新日期:2002-12-01 00:00:00

  • Telomerase and ATM/Tel1p protect telomeres from nonhomologous end joining.

    abstract::Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Chan SW,Blackburn EH

    更新日期:2003-05-01 00:00:00

  • Functional interdependence at the chromatin level between the MKK6/p38 and IGF1/PI3K/AKT pathways during muscle differentiation.

    abstract::During muscle regeneration, the mechanism integrating environmental cues at the chromatin of muscle progenitors is unknown. We show that inflammation-activated MKK6-p38 and insulin growth factor 1 (IGF1)-induced PI3K/AKT pathways converge on the chromatin of muscle genes to target distinct components of the muscle tra...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Serra C,Palacios D,Mozzetta C,Forcales SV,Morantte I,Ripani M,Jones DR,Du K,Jhala US,Simone C,Puri PL

    更新日期:2007-10-26 00:00:00

  • Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation.

    abstract::Immune cell function depends on specific metabolic programs dictated by mitochondria, including nutrient oxidation, macromolecule synthesis, and post-translational modifications. Mitochondrial adaptations have been linked to acute and chronic inflammation, but the metabolic cues and precise mechanisms remain unclear. ...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Chi Z,Chen S,Xu T,Zhen W,Yu W,Jiang D,Guo X,Wang Z,Zhang K,Li M,Zhang J,Fang H,Yang D,Ye Q,Yang X,Lin H,Yang F,Zhang X,Wang D

    更新日期:2020-10-01 00:00:00

  • The pathway for DNA recognition and RNA integration by a group II intron retrotransposon.

    abstract::Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifica...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Aizawa Y,Xiang Q,Lambowitz AM,Pyle AM

    更新日期:2003-03-01 00:00:00

  • Methylation of Histone H3K79 by Dot1L Requires Multiple Contacts with the Ubiquitinated Nucleosome.

    abstract::Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章


    authors: Zhang Y,Kutateladze TG

    更新日期:2019-06-06 00:00:00

  • VE-cadherin signaling induces EB3 phosphorylation to suppress microtubule growth and assemble adherens junctions.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Komarova YA,Huang F,Geyer M,Daneshjou N,Garcia A,Idalino L,Kreutz B,Mehta D,Malik AB

    更新日期:2012-12-28 00:00:00

  • The Mcm2-7 complex has in vitro helicase activity.

    abstract::Helicases unwind duplex DNA ahead of the polymerases at the replication fork. However, the identity of the eukaryotic replicative helicase has been controversial; in vivo studies implicate the ring-shaped heterohexameric Mcm2-7 complex, although only a specific subset of Mcm subunits (Mcm467) unwind DNA in vitro. To a...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Bochman ML,Schwacha A

    更新日期:2008-07-25 00:00:00

  • Activation of the bacterial sensor kinase PhoQ by acidic pH.

    abstract::The Salmonellae PhoQ sensor kinase senses the mammalian phagosome environment to activate a transcriptional program essential for virulence. The PhoQ periplasmic domain binds divalent cations, forming bridges with inner membrane phospholipids to maintain PhoQ repression. PhoQ also binds and is activated by cationic an...

    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Prost LR,Daley ME,Le Sage V,Bader MW,Le Moual H,Klevit RE,Miller SI

    更新日期:2007-04-27 00:00:00