Abstract:
:The mRNA cap structure is bound by either the nuclear (CBC) or the cytoplasmic (eIF4F) cap binding complex. Following mRNA export, CBC must be exchanged for eIF4F in the cytoplasm. It is not known how this exchange occurs or how this RNP remodeling event is integrated with mRNA function. Here we report genetic and biochemical evidence that the yeast translation initiation factor eIF4G associates with CBC, and that eIF4E, the eIF4F component that binds both the cap and eIF4G, antagonizes this interaction. Furthermore, we find that CBC can stimulate translation in extracts containing an eIF4G protein deficient for eIF4E binding. These data suggest that eIF4E binding to the eIF4G-CBC complex on newly exported mRNA displaces CBC, and that the first round of translation on mRNA may occur via a different mechanism than subsequent rounds.
journal_name
Mol Celljournal_title
Molecular cellauthors
Fortes P,Inada T,Preiss T,Hentze MW,Mattaj IW,Sachs ABsubject
Has Abstractpub_date
2000-07-01 00:00:00pages
191-6issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(05)00003-1journal_volume
6pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::HIV-1 Nef plays important roles in HIV-1 replication and pathogenesis. It is translated from completely spliced HIV-1 RNA, and its expression is inherently regulated at the levels of viral DNA transcription and RNA splicing. Here we show that Sam68 cytoplasmic mutants potently suppress Nef expression. The suppression ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.11.024
更新日期:2009-01-16 00:00:00
abstract::The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.10.022
更新日期:2005-12-22 00:00:00
abstract::The activity of RING finger ubiquitin ligases (E3) is dependent on their ability to facilitate transfer of ubiquitin from ubiquitin-conjugating enzymes (E2) to substrates. The G2BR domain within the E3 gp78 binds selectively and with high affinity to the E2 Ube2g2. Through structural and functional analyses, we determ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.05.010
更新日期:2009-06-26 00:00:00
abstract::BRCA1 functions at two distinct steps during homologous recombination (HR). Initially, it promotes DNA end resection, and subsequently it recruits the PALB2 and BRCA2 mediator complex, which stabilizes RAD51-DNA nucleoprotein filaments. Loss of 53BP1 rescues the HR defect in BRCA1-deficient cells by increasing resecti...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.12.010
更新日期:2019-03-21 00:00:00
abstract::Alternative splicing (AS) is a widespread process underlying the generation of transcriptomic and proteomic diversity and is frequently misregulated in human disease. Accordingly, an important goal of biomedical research is the development of tools capable of comprehensively, accurately, and efficiently profiling AS. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.08.018
更新日期:2018-10-04 00:00:00
abstract::Growing resistance of pathogenic bacteria and shortage of antibiotic discovery platforms challenge the use of antibiotics in the clinic. This threat calls for exploration of unconventional sources of antibiotics and identification of inhibitors able to eradicate resistant bacteria. Here we describe a different class o...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.03.001
更新日期:2018-04-05 00:00:00
abstract::Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.02.007
更新日期:2006-03-17 00:00:00
abstract::Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encod...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.11.020
更新日期:2015-01-22 00:00:00
abstract::BRAF is an oncogenic protein kinase that drives cell growth and proliferation through the MEK-ERK signaling pathway. BRAF inhibitors have demonstrated antitumor efficacy in melanoma therapy but have also been found to be associated with the development of cutaneous squamous cell carcinomas (cSCCs) in certain patients....
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.08.044
更新日期:2013-10-24 00:00:00
abstract::In this issue, Takahara and Maeda (2012) discover that together, Pbp1 and sequestration of the TORC1 complex in cytoplasmic mRNP stress granules provides a negative regulatory mechanism for TORC1 signaling during stress. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2012.07.005
更新日期:2012-07-27 00:00:00
abstract::The Mre11/Rad50/Nbs1 complex initiates double-strand break repair by homologous recombination (HR). Loss of Mre11 or its nuclease activity in mouse cells is known to cause genome aberrations and cellular senescence, although the molecular basis for this phenotype is not clear. To identify the origin of these defects, ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.10.011
更新日期:2016-11-03 00:00:00
abstract::The MHC class I antigen presentation system enables T cell immunosurveillance of cancers and viruses. A substantial fraction of the immunopeptidome derives from rapidly degraded nascent polypeptides (DRiPs). By knocking down each of the 80 ribosomal proteins, we identified proteins that modulate peptide generation wit...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.12.020
更新日期:2019-03-21 00:00:00
abstract::Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combinatio...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.08.025
更新日期:2007-11-09 00:00:00
abstract::Xenopus oocytes can epigenetically reprogram mouse somatic cells toward totipotency. In this issue, Jullien et al. (2014) now describe rapid, interdependent molecular events that facilitate this reprogramming. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2014.08.008
更新日期:2014-08-21 00:00:00
abstract::Nonsense-mediated decay (NMD) degrades mRNAs containing a premature termination codon (PTC). PTCs are a frequent cause of human genetic diseases, and the NMD pathway is known to modulate disease severity. Since partial NMD attenuation can potentially enhance nonsense suppression therapies, better definition of human-s...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.11.032
更新日期:2017-01-05 00:00:00
abstract::DNA methylation directed by 24-nucleotide (nt) small interfering RNAs (siRNAs) plays critical roles in gene regulation and transposon silencing in Arabidopsis. 24-nt siRNAs are known to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4). Here we report a disti...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.11.015
更新日期:2016-01-21 00:00:00
abstract::A major unresolved question in messenger RNA translation is how ribosomal release factors terminate protein synthesis. Class 1 release factors decode stop codons and trigger hydrolysis of the bond between the nascent polypeptide and tRNA some 75 A away from the decoding site. While the gross features of the release fa...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.06.032
更新日期:2007-09-07 00:00:00
abstract::In this issue of Molecular Cell, Mariner et al. (2008) demonstrate that Alu RNA from a human SINE represses RNA polymerase II transcription during heat shock. This noncoding RNA is the first example of a "protein-like" transcription factor with a distinct modular architecture. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2008.02.001
更新日期:2008-02-29 00:00:00
abstract::Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, w...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.01.040
更新日期:2013-02-21 00:00:00
abstract::Plant pattern recognition receptors (PRRs) perceive microbial and endogenous molecular patterns to activate immune signaling. The cytoplasmic kinase BIK1 acts downstream of multiple PRRs as a rate-limiting component, whose phosphorylation and accumulation are central to immune signal propagation. Previous work identif...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.12.026
更新日期:2018-02-01 00:00:00
abstract::Fidaxomicin is an antibacterial drug in clinical use for treatment of Clostridium difficile diarrhea. The active ingredient of fidaxomicin, lipiarmycin A3 (Lpm), functions by inhibiting bacterial RNA polymerase (RNAP). Here we report a cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme in complex with Lpm...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.02.026
更新日期:2018-04-05 00:00:00
abstract::Two publications in the current issue of Cell introduce novel methods for high-throughput single-cell transcriptomics by using droplet microfluidics and sophisticated barcoding schemes for transcriptional profiling of thousands of individual cells. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2015.05.019
更新日期:2015-05-21 00:00:00
abstract::S-adenosylmethionine (SAM) is the methyl-donor substrate for DNA and histone methyltransferases that regulate epigenetic states and subsequent gene expression. This metabolism-epigenome link sensitizes chromatin methylation to altered SAM abundance, yet the mechanisms that allow organisms to adapt and protect epigenet...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.03.004
更新日期:2020-04-16 00:00:00
abstract::Despite being sessile organisms constantly exposed to potential pathogens and pests, plants are surprisingly resilient to infections. Plants can detect invaders via the recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Plant PRRs are surface-localized receptor-like ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2014.03.028
更新日期:2014-04-24 00:00:00
abstract::Activation of p53 by DNA damage results in either cell-cycle arrest, allowing DNA repair and cell survival, or induction of apoptosis. As these opposite outcomes are both mediated by p53 stabilization, additional mechanisms to determine this decision must exist. Here, we show that glycogen synthase kinase-3 (GSK-3) is...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.03.033
更新日期:2011-06-10 00:00:00
abstract::Phase-separated states of proteins underlie ribonucleoprotein (RNP) granules and nuclear RNA-binding protein assemblies that may nucleate protein inclusions associated with neurodegenerative diseases. We report that the N-terminal low-complexity domain of the RNA-binding protein Fused in Sarcoma (FUS LC) is structural...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.09.006
更新日期:2015-10-15 00:00:00
abstract::We have analyzed two residues in the helicase domain of the E1 initiator protein. These residues are part of a highly conserved structural motif, the beta-hairpin, which is present in the helicase domain of all papovavirus initiator proteins. These proteins are unique in their ability to transition from local template...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.02.009
更新日期:2007-03-23 00:00:00
abstract::Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS d...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80076-3
更新日期:1998-04-01 00:00:00
abstract::Takahashi et al. (2018) report that the peptide CLE25 together with the BAM1, BAM3 LRR receptor-like kinases are involved in root-to-shoot communication during dehydration stress in Arabidopsis. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2018.06.011
更新日期:2018-06-21 00:00:00
abstract::Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.07.013
更新日期:2009-09-11 00:00:00