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Ribosome components are associated with sites of transcription.

Abstract：

:It is generally believed that eukaryotic ribosomes first associate with mRNA in the cytoplasm. However, we show with chromosomal immunostaining and in situ hybridization that ribosomal subunits are present at transcription sites of Drosophila salivary gland chromosomes. Immunostaining was carried out with antibodies specific for 27 ribosomal proteins, two translation factors and one that specifically recognizes rRNA. In situ hybridization was with several probes specific for both rRNA subunits. The kinetics of recruitment following transcription initiation suggest that the association is with newly transcribed pol II transcripts. These data indicate that ribosome components associate with nascent RNP complexes within the nucleus.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Brogna S,Sato TA,Rosbash M

subject

Has Abstract

pub_date

2002-07-01 00:00:00

pages

93-104

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097276502005658

journal_volume

10

pub_type

杂志文章

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