Abstract:
:The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabled high-throughput genetic and epigenetic studies. These high-throughput approaches offer investigators a toolkit for functional interrogation of not only protein-coding genes but also noncoding DNA. Historically, noncoding DNA has lacked the detailed characterization that has been applied to protein-coding genes in large part because there has not been a robust set of methodologies for perturbing these regions. Although the majority of high-throughput CRISPR screens have focused on the coding genome to date, an increasing number of CRISPR screens targeting noncoding genomic regions continue to emerge. Here, we review high-throughput CRISPR-based approaches to uncover and understand functional elements within the noncoding genome and discuss practical aspects of noncoding library design and screen analysis.
journal_name
Mol Celljournal_title
Molecular cellauthors
Montalbano A,Canver MC,Sanjana NEdoi
10.1016/j.molcel.2017.09.017subject
Has Abstractpub_date
2017-10-05 00:00:00pages
44-59issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(17)30668-8journal_volume
68pub_type
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