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Genomic Copy-Number Loss Is Rescued by Self-Limiting Production of DNA Circles.

Abstract：

:Copy-number changes generate phenotypic variability in health and disease. Whether organisms protect against copy-number changes is largely unknown. Here, we show that Saccharomyces cerevisiae monitors the copy number of its ribosomal DNA (rDNA) and rapidly responds to copy-number loss with the clonal amplification of extrachromosomal rDNA circles (ERCs) from chromosomal repeats. ERC formation is replicative, separable from repeat loss, and reaches a dynamic steady state that responds to the addition of exogenous rDNA copies. ERC levels are also modulated by RNAPI activity and diet, suggesting that rDNA copy number is calibrated against the cellular demand for rRNA. Last, we show that ERCs reinsert into the genome in a dosage-dependent manner, indicating that they provide a reservoir for ultimately increasing rDNA array length. Our results reveal a DNA-based mechanism for rapidly restoring copy number in response to catastrophic gene loss that shares fundamental features with unscheduled copy-number amplifications in cancer cells.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Mansisidor A,Molinar T Jr,Srivastava P,Dartis DD,Pino Delgado A,Blitzblau HG,Klein H,Hochwagen A

doi

10.1016/j.molcel.2018.08.036

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

583-593.e4

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(18)30696-8

journal_volume

72

pub_type

杂志文章

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