Abstract：
:The hairpin ribozyme is a self-cleaving motif found in the negatives strand of the satellite RNA of some plant viruses. In its natural context, the ribozyme comprises four helices, two of which contain conserved formally unpaired loops, that are adjacent arms of a four-way RNA junction. We show that the arms that would carry these loops are brought close together in the global conformation of the isolated junction. Using fluorescence resonance energy transfer, we demonstrate a two-magnesium ion-dependent conformational transition of the complete ribozyme that brings the loopbearing arms into close physical proximity. The ribozyme is active as a four-way junction, and the rate of cleavage may be modulated by the conformation of the four-way junction.
journal_name
Mol Celljournal_title
Molecular cellauthors
Murchie AI,Thomson JB,Walter F,Lilley DMdoi
10.1016/s1097-2765(00)80086-6subject
Has Abstractpub_date
1998-05-01 00:00:00pages
873-81issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(00)80086-6journal_volume
1pub_type
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