Abstract:
:Necroptosis is an important form of lytic cell death triggered by injury and infection, but whether mixed lineage kinase domain-like (MLKL) is sufficient to execute this pathway is unknown. In a genetic selection for human cell mutants defective for MLKL-dependent necroptosis, we identified mutations in IPMK and ITPK1, which encode inositol phosphate (IP) kinases that regulate the IP code of soluble molecules. We show that IP kinases are essential for necroptosis triggered by death receptor activation, herpesvirus infection, or a pro-necrotic MLKL mutant. In IP kinase mutant cells, MLKL failed to oligomerize and localize to membranes despite proper receptor-interacting protein kinase-3 (RIPK3)-dependent phosphorylation. We demonstrate that necroptosis requires IP-specific kinase activity and that a highly phosphorylated product, but not a lowly phosphorylated precursor, potently displaces the MLKL auto-inhibitory brace region. These observations reveal control of MLKL-mediated necroptosis by a metabolite and identify a key molecular mechanism underlying regulated cell death.
journal_name
Mol Celljournal_title
Molecular cellauthors
Dovey CM,Diep J,Clarke BP,Hale AT,McNamara DE,Guo H,Brown NW Jr,Cao JY,Grace CR,Gough PJ,Bertin J,Dixon SJ,Fiedler D,Mocarski ES,Kaiser WJ,Moldoveanu T,York JD,Carette JEdoi
10.1016/j.molcel.2018.05.010subject
Has Abstractpub_date
2018-06-07 00:00:00pages
936-948.e7issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(18)30360-5journal_volume
70pub_type
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