Abstract：
:Transcriptional and posttranslational signals are known mechanisms that promote efficient responses to DNA damage. We have identified Saccharomyces cerevisiae tRNA methyltransferase 9 (Trm9) as an enzyme that prevents cell death via translational enhancement of DNA damage response proteins. Trm9 methylates the uridine wobble base of tRNAARG(UCU) and tRNAGLU(UUC). We used computational and molecular approaches to predict that Trm9 enhances the translation of some transcripts overrepresented with specific arginine and glutamic acid codons. We found that translation elongation factor 3 (YEF3) and the ribonucleotide reductase (RNR1 and RNR3) large subunits are overrepresented with specific arginine and glutamic acid codons, and we demonstrated that Trm9 significantly enhances Yef3, Rnr1, and Rnr3 protein levels. Additionally, we identified 425 genes, which included YEF3, RNR1, and RNR3, with a unique codon usage pattern linked to Trm9. We propose that Trm9-specific tRNA modifications enhance codon-specific translation elongation and promote increased levels of key damage response proteins.
journal_name
Mol Celljournal_title
Molecular cellauthors
Begley U,Dyavaiah M,Patil A,Rooney JP,DiRenzo D,Young CM,Conklin DS,Zitomer RS,Begley TJdoi
10.1016/j.molcel.2007.09.021subject
Has Abstractpub_date
2007-12-14 00:00:00pages
860-70issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(07)00633-8journal_volume
28pub_type
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